Possible role of hydrogen sulfide as an endogenous relaxation factor in the rat bladder and prostate

Zou, Suo; Shimizu, Takahiro; Shimizu, Shogo; Higashi, Youichirou; Nakamura, Kumiko; Ono, Hideaki; Aratake, Takaaki; Saito, Motoaki

doi:10.1002/nau.23788

Cited by 17 publications

(25 citation statements)

References 25 publications

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“…In urethane‐anesthetized (0.8 g/kg, i.p.) rats, a catheter was inserted into the bladder via the BL‐D 5,12 . After stabilization for 60 min, cystometry was performed with saline at a constant flow rate (2.4 mL/h) until four to five voidings were observed.…”

Section: Methodsmentioning

confidence: 99%

“…Bladder (BL‐D and BL‐T) strips were prepared with approximately 1 mm × 5 mm dimensions 5 . We mounted these strips in Krebs‐Henseleit buffer (10 mL), bubbled with 5% CO 2 and 95% O 2 (37°C).…”

Section: Methodsmentioning

confidence: 99%

“…We observed that endogenous H 2 S, CAT and MPST were present in the bladder (BL‐D and BL‐T) of Wistar rats. A rapid‐releasing H 2 S donor NaHS relaxed the precontracted bladder (BL‐D and BL‐T) strips, and the intravesical instillation of a slow‐releasing H 2 S donor GYY4137 prolonged ICIs 5 . Therefore, H 2 S can be considered to be an endogenous relaxation factor in the Wistar rat bladder.…”

Section: Introductionmentioning

confidence: 98%

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Age‐related differences in responses to hydrogen sulfide in the bladder of spontaneously hypertensive rats

Zou¹,

Shimizu²,

Yamamoto³

et al. 2021

Int J of Urology

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To investigate whether a response to hydrogen sulfide donors (GYY4137 and sodium hydrosulfide) and the endogenous hydrogen sulfide system (hydrogen sulfide level and expression of cysteine aminotransferase, cystathionine b-synthase, cystathionine c-lyase, and 3-mercaptopyruvate sulfurtransferase) in the spontaneously hypertensive rat bladder differ with age, we compared the responses of hydrogen sulfide donors to micturition and bladder relaxation, and the endogenous hydrogen sulfide system in the bladder of 18-week versus 12-week-old spontaneously hypertensive rats. Methods: GYY4137 was intravesically administered and cystometry was performed in anesthetized rats. The responses of sodium hydrosulfide were evaluated in carbacholmediated precontracted bladder strips. Bladder hydrogen sulfide levels and expression levels of each enzyme were investigated using the methylene blue method and Western blotting, respectively. Results: GYY4137 treatment significantly prolonged intercontraction intervals only in 12-week-old rats. Sodium hydrosulfide-induced bladder relaxation was significantly attenuated in the strips of 18-week-old rats compared with that in 12-week-old rats. In the bladder dome, significant increases in hydrogen sulfide levels and in the expression of cystathionine b-synthase, 3-mercaptopyruvate sulfurtransferase, and cysteine aminotransferase were observed in 18-week-old rats compared with 12-week-old rats. However, cystathionine c-lyase bands were not detected in bladder tissues of either group. Conclusions: Bladder relaxation induced by hydrogen sulfide may be attenuated in spontaneously hypertensive rats in an age-dependent manner.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Age‐related differences in responses to hydrogen sulfide in the bladder of spontaneously hypertensive rats

Zou¹,

Shimizu²,

Yamamoto³

et al. 2021

Int J of Urology

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“…These compounds include pyruvate, which can be converted into acetyl-CoA and enter the tricarboxylic acid (TCA) cycle or be used for fatty acid synthesis, and α-ketoglutarate, a precursor of glutamate and an intermediate of the TCA cycle. [48][49][50][51] Moreover, through the action of two of the enzymes involved in the production of H 2 S-cysteine aminotransferase (CAT) and 3mercaptopyruvate sulphurtransferase (3-MST)-cysteine is sequentially converted into 3-mercaptopyruvate (3-MP) with the release of an amino group that will react with α-ketoglutarate, ending with the formation of glutamate and pyruvate ( Fig. 3a), again connecting cysteine metabolism with the TCA cycle.…”

Section: Cysteine Degradation and Energy Metabolismmentioning

confidence: 99%

Cysteine metabolic circuitries: druggable targets in cancer

et al. 2020

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To enable survival in adverse conditions, cancer cells undergo global metabolic adaptations. The amino acid cysteine actively contributes to cancer metabolic remodelling on three different levels: first, in its free form, in redox control, as a component of the antioxidant glutathione or its involvement in protein s-cysteinylation, a reversible post-translational modification; second, as a substrate for the production of hydrogen sulphide (H2S), which feeds the mitochondrial electron transfer chain and mediates per-sulphidation of ATPase and glycolytic enzymes, thereby stimulating cellular bioenergetics; and, finally, as a carbon source for epigenetic regulation, biomass production and energy production. This review will provide a systematic portrayal of the role of cysteine in cancer biology as a source of carbon and sulphur atoms, the pivotal role of cysteine in different metabolic pathways and the importance of H2S as an energetic substrate and signalling molecule. The different pools of cysteine in the cell and within the body, and their putative use as prognostic cancer markers will be also addressed. Finally, we will discuss the pharmacological means and potential of targeting cysteine metabolism for the treatment of cancer.

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“…The usefulness of cysteine as a carbon source is visible along the cysteine catabolic pathway, since cysteine catabolism originates organic compounds used in carbon and energy metabolism ( 19 – 23 ).…”

Section: Cysteine As a Carbon Source In Cancermentioning

confidence: 99%

Cysteine as a Carbon Source, a Hot Spot in Cancer Cells Survival

Serpa

2020

Front. Oncol.

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Cancer cells undergo a metabolic rewiring in order to fulfill the energy and biomass requirements. Cysteine is a pivotal organic compound that contributes for cancer metabolic remodeling at three different levels: (1) in redox control, free or as a component of glutathione; (2) in ATP production, via hydrogen sulfide (H 2 S) production, serving as a donor to electron transport chain (ETC), and (3) as a carbon source for biomass and energy production. In the present review, emphasis will be given to the role of cysteine as a carbon source, focusing on the metabolic reliance on cysteine, benefiting the metabolic fitness and survival of cancer cells. Therefore, the interplay between cysteine metabolism and other metabolic pathways, as well as the regulation of cysteine metabolism related enzymes and transporters, will be also addressed. Finally, the usefulness of cysteine metabolic route as a target in cancer treatment will be highlighted.

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Possible role of hydrogen sulfide as an endogenous relaxation factor in the rat bladder and prostate

Abstract: It is possible that H S can function as an endogenous relaxation factor in the rat bladder and prostate.

Cited by 17 publications

References 25 publications

Age‐related differences in responses to hydrogen sulfide in the bladder of spontaneously hypertensive rats

Age‐related differences in responses to hydrogen sulfide in the bladder of spontaneously hypertensive rats

Cysteine metabolic circuitries: druggable targets in cancer

Cysteine as a Carbon Source, a Hot Spot in Cancer Cells Survival

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