Possible Resuscitation of Liver Function by Hypothermic Reperfusion in Vitro After Prolonged (24-Hour) Cold Preservation—a 31p NMR Study

Bj, Fuller; Al, Busza; Proctor, E

doi:10.1097/00007890-199009000-00030

Cited by 55 publications

(23 citation statements)

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“…the functional assessment of hepatic failure in patients prior to orthotopic liver transplantation (OLT) the assessment of the viability of the isolated, stored donor liver before implantation into the recipient the early diagnosis of rejection post-OLT [5][6][7][8][9][10][11][12][13][14][15][16] .…”

Section: Applications Of Magnetic Resonance Spectroscopy To Liver Tramentioning

confidence: 99%

Applications of magnetic resonance spectroscopy to chronic liver disease

Taylor-Robinson

2001

Clinical Medicine

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-In vivo magnetic resonance spectroscopy (MRS) provides a non-invasive 'window' on biochemical processes within the body. The technique is currently a research tool, but new developments in whole-body magnetic resonance imaging (MRI) may provide a role for clinical MRS in obtaining functional information at the end of a standard MRI examination. Applications of hepatic and cerebral MRS to chronic liver disease and its associated complications are specifically considered in this article. Changes in phosphorus-31 MRS with the underlying functional severity of the cirrhotic liver are discussed. These reflect increased turnover of cell membranes as the liver attempts to regenerate. Patterns of spectral abnormality in the transplanted liver are described, and also the potential use of the technique to assess the viability of the stored donor liver in the time period between organ harvesting and implantation in the transplant recipient. Metabolite abnormalities in the brain are defined in hepatic encephalopathy and in patients infected with the hepatitis C virus who have minimal hepatic inflammation. Future trends are considered: for example, the use of MRS as a non-invasive tool to assess the effectiveness of liver-directed gene therapy.

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Section: Applications Of Magnetic Resonance Spectroscopy To Liver Tramentioning

confidence: 99%

Applications of magnetic resonance spectroscopy to chronic liver disease

Taylor-Robinson

2001

Clinical Medicine

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“…The reperfusion-induced reoxy genation of a graft, which initiates the recov ery of mitochondrial energy metabolism in vivo, is simulated in the test procedure by reoxygenation in vitro of mitochondria ob tained from specimens of the ischemically stored organ. The concept as well as the short period of time for the measurement and the small size of the tissue specimen should en courage researchers to check the usefulness of this method for the determination of the via bility of stored organs: graft viability is ideally to be determined before implantation and without in vitro perfusion, which may com prise hazards [16][17][18].…”

Section: Discussionmentioning

confidence: 99%

Rapid Determination of the Membrane Potential of Mitochondria in Small Biopsy Specimens of the Liver

et al. 1995

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In liver transplantation, graft viability is ideally to be determined before implantation. Integrity of mitochondria may be a prerequisite to a viable graft. A new method is presented, which allows for the determination of the membrane potential of mitochondria (MPM; mV) in state 4 respiration within 50 min in 40-mg specimens, employing rhodamine 123 as a probe. Normal control showed a MPM of 239.2 mV. Storage in saline at 37°C yielded an impaired MPM of 153.5 mV within 3 h. The cold storage at 1 °C could preserve MPM at quasi-normal after 3 h but reduced it significantly after 24 h to 222.2 mV in saline (p < 0.005 vs. control) and 231.0 mV in UW solution (p < 0.05 vs. control): the difference between the 24-hour values was significant (p < 0.05).

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“…They reported good maintenance of function in a liver perfused with an acellular, oxygenated perfusate at 15ºC, just on the edge of the profound hypothermia temperature range. Several studies demonstrated that perfusion with oxygenated solutions prevented ATP loss and avoided the injurious ischemic cascades that are set into motion after ATP depletion [7,8,9,10]. The role of oxygenation has also been supported by other studies [11,12,13,14].…”

Section: Introductionmentioning

confidence: 95%

Ex vivo evaluation of porcine livers post-hypothermic machine perfusion at 4-6ºC and 12-14ºC

Brockbank¹,

Lee²,

Greene³

et al. 2012

J Regen Med Tissue Eng

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Background: The goal of our research is the development of a clinical hypothermic machine perfusion method and device for liver preservation during storage and transport prior to transplantation. The purpose of this study was comparison of two hypothermic temperature ranges. Methods: Heart beating donor pig livers with 2h of static cold storage on ice were employed. Thirteen experimental livers were perfused, at either 4-6ºC or 12-14ºC with oxygenation, employing a prototype device. They were compared with six control 2h static cold stored livers during normothermic ex vivo blood perfusion. Physiological measurements and biochemical perfusate analyses were performed to assess the impact of storage conditions on liver functions. Statistics were assessed by one way analysis of variance, p<0.05 was regarded as significant. Results: During hypothermic perfusion perfusate solutes (Na

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Possible Resuscitation of Liver Function by Hypothermic Reperfusion in Vitro After Prolonged (24-Hour) Cold Preservation—a 31p NMR Study

Cited by 55 publications

References 0 publications

Applications of magnetic resonance spectroscopy to chronic liver disease

Applications of magnetic resonance spectroscopy to chronic liver disease

Rapid Determination of the Membrane Potential of Mitochondria in Small Biopsy Specimens of the Liver

Ex vivo evaluation of porcine livers post-hypothermic machine perfusion at 4-6ºC and 12-14ºC

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