Possible relationship between mitochondrial changes and oxidative stress under low dose-rate irradiation

Meng, Qingmei; Zaharieva, Elena Karamfilova; Sasatani, Megumi; Kobayashi, Junya

doi:10.1080/13510002.2021.1971363

Cited by 22 publications

(19 citation statements)

References 58 publications

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“…The dose‐rate was set close to the lower limit of the intermediate range (1 mGy/min) so that we could examine whether the novel method is suitable for analyzing the biological effect of relatively low dose‐rate irradiation. A previous study has shown that, under the same irradiation conditions, ROS staining by 2′,7′‐dichlorofluorescein diacetate (DCFH‐A) detected an increase of ~20% in ROS generation in 48BRhTERT cells (Meng et al, 2021). The same study suggested that DNA double‐strand breaks occurred at a much‐reduced rate compared with that in cells irradiated with the same dose at a higher dose‐rate (0.9 Gy/min).…”

Section: Introductionmentioning

confidence: 99%

Analysis method of cellular stress caused by intermediate dose‐rate irradiation using a cell lysate array technique

et al. 2023

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Ionizing radiation damages DNA and may lead to the development of cancer.Irradiation also generates reactive oxygen species (ROS) which cause damage to various biological molecules. Relatively low dose-rate irradiation causes less damage. However, the damage and its effects on cell fate are difficult to evaluate. To develop a method to analyze the damage and accompanying changes in physiology in cells irradiated by γ-rays at a relatively low dose-rate, we used the protein array technique to quantify marker proteins involved in the stress response and the regulation of cell growth and death. This method enabled efficient analyses of many replicates of experimental data on cell lysate samples. We detected relatively small changes in the levels of these proteins in the irradiated cells. Changes in protein levels suggested ROS production and DNA damage as well as cell cycle retardation and the progression of cellular senescence. Thus, our approach shows promise for analyzing the biological effects of relatively low dose-rate irradiation.

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Section: Introductionmentioning

confidence: 99%

Analysis method of cellular stress caused by intermediate dose‐rate irradiation using a cell lysate array technique

et al. 2023

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“…When IR interacts with organisms, it may directly or indirectly cause DNA lesions. The direct interaction is caused by ions hitting on DNA molecules, while the indirect effect is caused by reactive oxygen species (ROS) aggregation through the radiolysis of water ( van de Walle et al, 2016 ; Gudkov et al, 2019 ; Choi et al, 2021 ; Meng et al, 2021 ). ROS can diffuse through the cells and damage their components, but they are also important signaling molecules in plants responses to stresses, triggering related response mechanisms ( Volkova et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

The Mutational, Epigenetic, and Transcriptional Effects Between Mixed High-Energy Particle Field (CR) and 7Li-Ion Beams (LR) Radiation in Wheat M1 Seedlings

Zhao

Zhang

et al. 2022

Front. Plant Sci.

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Ionizing radiation (IR) is an effective approach for mutation breeding. Understanding the mutagenesis and transcriptional profiles induced by different mutagens is of great significance for improving mutation breeding efficiency. Here, using RNA sequencing and methylation-sensitive amplification polymorphism (MSAP) approaches, we compared the genetic variations, epigenetics, and transcriptional responses induced by the mixed high-energy particle field (CR) and 7Li-ion beam (LR) radiation in M1 seedlings of two wheat genotypes (Yangmai 18 and Yangmai 20). The results showed that, in both wheat genotypes, CR displayed significantly a higher mutation efficiency (1.79 × 10–6/bp) than that by LR (1.56 × 10–6/bp). The induced mutations were not evenly distributed across chromosomes and varied across wheat genotypes. In Y18 M1, the highest number of mutations were detected on Chr. 6B and Chr. 6D, whilst in Y20 M1, Chr. 7A and Chr. 3A had the highest mutations. The transcript results showed that total of 4,755 CR-regulated and 1,054 LR-regulated differentially expressed genes (DEGs) were identified in the both genotypes. Gene function enrichment analysis of DEGs showed that these DEGs overlapped or diverged in the cascades of molecular networks involved in “phenylpropanoid biosynthesis” and “starch and sucrose metabolism” pathways. Moreover, IR type specific responses were observed between CR an LR irradiation, including specific TFs and response pathways. MSAP analysis showed that DNA methylation level increased in LR treatment, while decreased at CR. The proportion of hypermethylation was higher than that of hypomethylation at LR, whereas a reverse pattern was observed at CR, indicating that DNA methylation plays critical roles in response to IR irradiation. All these results support that the response to different IRs in wheat includes both common and unique pathways, which can be served as a useful resource to better understand the mechanisms of responses to different IRs in other plants.

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“…[8] They are essential for normal physiological functions, but excess ROS leads to oxidative stress causing cell injury and even cell death. [9] Recent evidence has demonstrated that mitochondrial dynamics are tightly coupled to ROS generation. [10] Aberrant mitochondrial morphology may lead to ROS overaccumulation, which in turn, may cause mitochondrial damage and disruption of mitochondrial dynamics.…”

Section: Introductionmentioning

confidence: 99%

“…Reactive oxygen species (ROS), mainly generated as a by‐product of mitochondrial respiration or metabolism, play a role in both cell progression and survival [8] . They are essential for normal physiological functions, but excess ROS leads to oxidative stress causing cell injury and even cell death [9] . Recent evidence has demonstrated that mitochondrial dynamics are tightly coupled to ROS generation [10] .…”

Section: Introductionmentioning

confidence: 99%

Synthesis and in Vivo Evaluation of Triphenylphosphonium Conjugated Trimetazidine with Enhanced Cardioprotection and Ability to Restore Mitochondrial Function

Jia

et al. 2022

Chemistry & Biodiversity

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Trimetazidine exhibits great therapeutic potential in cardiovascular diseases and mitochondria-mediated cardioprotection by trimetazidine has been widely reported. In this study, to enhance its cardioprotection, the triphenylphosphonium-based modification of trimetazidine was conducted to deliver it specifically to mitochondria. Fifteen triphenylphosphonium (TPP) conjugated trimetazidine analogs were designed and synthesized. Their protective effects were evaluated in vivo using a tert-butyl hydroperoxide (t-BHP) induced zebrafish injury model. Structure-activity relationship correlations revealed the best way to couple the TPP moiety to trimetazidine, and led to a new conjugate (18a) with enhanced therapeutic properties. Compared to trimetazidine, 18a effectively protects against heart injury in the zebrafish model at a much lower concentration. Further study in t-BHP treated zebrafish and H9c2 cells demonstrated that 18a protects against cardiomyocyte death and damage by inhibiting excessive production of ROS, maintaining mitochondrial morphology, and preventing mitochondrial dysfunction. Consequently, 18a can be regarded as a potential therapeutic agent for cardioprotection.

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Possible relationship between mitochondrial changes and oxidative stress under low dose-rate irradiation

Cited by 22 publications

References 58 publications

Analysis method of cellular stress caused by intermediate dose‐rate irradiation using a cell lysate array technique

Analysis method of cellular stress caused by intermediate dose‐rate irradiation using a cell lysate array technique

The Mutational, Epigenetic, and Transcriptional Effects Between Mixed High-Energy Particle Field (CR) and 7Li-Ion Beams (LR) Radiation in Wheat M1 Seedlings

Synthesis and in Vivo Evaluation of Triphenylphosphonium Conjugated Trimetazidine with Enhanced Cardioprotection and Ability to Restore Mitochondrial Function

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