Possible Protective Effect of Nigella Sativa Oil Against Piroxicam-Induced Gastric Mucosal Damage in Adult Male Albino Rats (Light and Scanning Electron Microscopic Study)

Mohammed, Sally S.; Naim, Magda Mohamed; Gawad, Sahar khalil Abdel; Mahmoud, Somaya

doi:10.1097/00767537-201003000-00013

Cited by 7 publications

(10 citation statements)

References 4 publications

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“…This was evidenced by sloughing of surface epithelium, exfoliated cells and distorted fundic glandular architecture with dilatation of some gastric glands. These results were in agreement with another study [17] that reported superficial erosions in the gastric mucosa, remnants of the gastric glands appearing in the lumen, surface mucous cells with lightly stained cytoplasm and nuclear swelling, many mucous neck and chief cells with necrotic changes after oral administration of piroxicam (another NSAID) for 6 hours.…”

Section: Discussionsupporting

confidence: 93%

“…In the current work, the PAS-stained sections of the aspirin-induced ulcer group showed a statistically significant decrease of PAS positive reaction, with a thin interrupted PAS positive surface mucous film and moderate PAS positive mucus in cells lining the gastric pits. Similar to those results, it was demonstrated [17] that ulcer group showed damaged gastric mucosa with marked decrease of the PAS positive mucus in the gastric pits and extending down into the lumen of the gland, while the neck region showed PAS positive mucus. They suggested that these changes were due to suppressed PGs production and damage of the surface epithelial cells and mucous neck cells which leads to decreased mucous production.…”

Section: Discussionsupporting

confidence: 86%

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Comparative Histological and immunohistochemical study on the effect of curcumin and wild honey versus omeprazole on a rat model of gastric ulcer

Elkalawy

Elkader

Ibrahim

et al. 2019

Journal of Medical Histology

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Background and objectives: Gastric ulcers are among the most common diseases affecting humans. Omeprazole, a proton pump inhibitor, is widely used in the treatment of peptic ulcer. Wild honey alone, or in combination with other natural products, has been used to treat gastric ulcer. This study aimed to investigate the possible curative role of curcumin combined with wild honey in comparison to omeprazole on aspirin-induced gastric mucosal damage in adult male albino rats. Methods and Results: Thirty adult male albino rats were divided into: Group1 (control group), group2 (ulcer group) (received 200mgkg BW aspirin orally). On day 6, rats of group2 were equally subdivided into; subgroup 2a (continued Aspirin), subgroup 2b (received combined curcumin and honey) and subgroup 2c (received 20mgkg BW omeprazole orally). All rats were sacrificed at day 11. Fundic specimens were processed for H &E, PAS, and immunohistochemical stain for COX-2, followed by morphometric assessment and statistical analysis. Aspirin-induced ulcer was evidenced by sloughing of surface epithelium, widened fundic glands, vacuolated surface and mucous neck cells and some vacuolated or shrunken oxyntic with pyknotic nuclei, in addition to significant increase in COX-2 immunoexpression. Combined curcumin and honey improved these histological alterations with a significant decrease in COX-2 immunoreactivity. Conclusion: the combination of curcumin and wild honey healed most of the deleterious morphological changes of aspirininduced gastric ulcer, with a comparable effect to omeprazole. This offers an alternative treatment of peptic ulcer to avoid adverse drug reactions.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Comparative Histological and immunohistochemical study on the effect of curcumin and wild honey versus omeprazole on a rat model of gastric ulcer

Elkalawy

Elkader

Ibrahim

et al. 2019

Journal of Medical Histology

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“…Previous studies illustrated the protective and therapeutic role of N sativa oil in various gastrointestinal disorders involving mucosal injury and cancers [14,15,16] . Subsequently, the present research aimed to study the effect of Fosamax on the histological structure of the duodenal mucosa in adult male albino rats and to evaluate the possible protective role of nigella sativa oil using different histological and immunohistochemical techniques.…”

Section: Introductionmentioning

confidence: 99%

Effect of Fosamax on the duodenal mucosa in adult male albino rats and the possible protection by nigella sativa oil: a histological and immunohistochemical study

Kassab

2019

Egyptian Journal of Histology

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Background: Fosamax, an oral bisphosphonate, is the most commonly prescribed drug for the treatment of various bones diseases. However, Fosamax therapy is associated with gastrointestinal toxicity that influences the treatment compliance. Nigella sativa (N sativa) is an annual herbaceous plant and have been safely used as a natural remedy in Egypt. It has a wide range of pharmacological activities and a proposed role in combating many gastrointestinal disorders. Aim: This work aimed to study the effect of Fosamax on the histological structure of the duodenal mucosa of albino rats and to evaluate the possible protective role of nigella sativa oil. Materials and methods: Forty adult male albino rats were divided into four equal groups: the control group, the N sativa oil-treated group (2.5 ml/kg), the Fosamax-treated group (0.05mg/kg), and both Fosamax and N sativa oil-treated group. Treatments were given orally once weekly for 13 weeks. Specimens from the duodenum were processed for light microscopy. Immunohistochemical study was carried out using antibodies against nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax) and Ki67 protein. Results: Specimens from Fosamax-treated rats showed a statistically significant decrease in the duodenal mucosal thickness. The lining epithelium showed nuclear alteration and vacuolated cytoplasm and there was mononuclear cellular infiltration. The immunohistochemical study of Fosamax-treated group showed a significant increase in NF-kB, COX-2 and Bax immunoreaction and a significant decrease in Bcl-2 and Ki67 expression. In contrast, minimal changes were observed in rats treated concomitantly with both Fosamax and N sativa oil with non-significant changes in the immunoreactions compared with the control group. Conclusion: Fosamax induced structural changes in the duodenal mucosa of adult albino rats, which could be ameliorated by concomitant treatment with nigella sativa oil.

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“…TNF-α is a proinflammatory cytokine that plays an important role in this process. The NF-ҡβ pathway is activated by TNF α, thymoquinone works to inhibit NF-ҡβ which is activated by TNF α and inhibits NF-ҡβ translocation to PDA cell nuclei (Ductal adenocarcinoma) pancreatic [20][21][22]. So that it can be explained that the administration of ethanol extract Nigella sativa in mice model of preeclampsia has been shown to reduce serum TNF-α levels.…”

Section: Positive Effects Of Black Cumin Ethanol Extract (Nigella Satmentioning

confidence: 99%

“…Thymoquinone works as an inflammatory inhibitor that works through the pathway as an antiinflammatory. The inflammatory immune response starts from the recruitment of inflammatory cells into the inflammatory lesion [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

The positive effect of black cumin ethanol extract (Nigella sativa) on decreasing serum TNF-α levels and interleukin-8 levels in mice model of preeclampsia

Zainiyah¹,

Noviani²,

Hipni³

2018

biomedicalresearch

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Background: During placental preeclampsia experiencing oxidative stress, oxidative stress can increase the expression and activation of nuclear factor-kappa B which regulates the levels of TNF α (Tumor necrosis factor-α), interleukin-6, interleukin-8, COX-2 (Cyclooxygenase-2) genes. Objective: Analyzing the positive effects of black cumin ethanol extract (Nigella sativa) on reducing serum TNF-α levels and serum interleukin-8 in mice model preeclampsia. Methods: Research design True experimental with type post-test only control group design. Research using pregnant Balb/c strain mice was healthy used as preeclampsia model mice and divided into 6 groups. Ethanol extract of seeds is Nigella sativa gave orally in the treatment group from 15 to 19 d at a dose of 500 mg kg/d, 1000 mg/kg/d. 1500 mg/kg/d, 2000 mg/kg body weight/d, and terminated at 20 th gestational age. Measurement of TNF-α levels and Interleukin-8 levels using ELISA (Enzyme-linked immune sorbent assay). Results: TNF-α levels and interleukin-8 levels showed a decreased level after being given treatment at a dose of 1500 mg/kg body weight. Conclusions: Black cumin extract has a positive effect to reduce serum TNF-α and serum interleukin-8 levels to be used as a therapy for preeclampsia disorders.

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Possible Protective Effect of Nigella Sativa Oil Against Piroxicam-Induced Gastric Mucosal Damage in Adult Male Albino Rats (Light and Scanning Electron Microscopic Study)

Cited by 7 publications

References 4 publications

Comparative Histological and immunohistochemical study on the effect of curcumin and wild honey versus omeprazole on a rat model of gastric ulcer

Comparative Histological and immunohistochemical study on the effect of curcumin and wild honey versus omeprazole on a rat model of gastric ulcer

Effect of Fosamax on the duodenal mucosa in adult male albino rats and the possible protection by nigella sativa oil: a histological and immunohistochemical study

The positive effect of black cumin ethanol extract (Nigella sativa) on decreasing serum TNF-α levels and interleukin-8 levels in mice model of preeclampsia

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