Possible Proliferative Impact of Human Platelet-Rich Fibrin Obtained From Controlled Type 2 Diabetic Patients on Human Dental Pulp Stem Cells (In Vitro Study)

Abstract

Purpose: This study was carried out in order to assess the effect of 10% PRF exudate obtained from controlled type 2 diabetic patients on proliferation capacity of dental pulp stem cells (DPSCs). Then 1% PRF exudate obtained from controlled type 2 diabetic patients was tested for osteogenic capability after 1 week. Material and methods: PRF exudates were prepared using the direct method from 8 healthy donors (control group) and 8 controlled type 2 diabetic patients (study group). In 96-well plates, DPSCs harvested from pulp tissue of impacted lower third molar were treated with 10% PRF exudate obtained from healthy donors and controlled type 2 diabetic patients for 1,3 and 5 days. The effects of exudates on proliferation and cell viability were tested using the MTS assay. For osteogenic capability, DPSCs were treated with 1%PRF exudate obtained from healthy donors and controlled type 2 diabetic patients supplemented with osteogenic medium for 1week and the osteodifferentiation assay was held using alizarin red stain with ELIZA reader. All data were subjected into one-way Anova statistical analysis. Results: The concentrations of 10% and 1% PRF exudates obtained from controlled type 2 diabetic patients had proliferative and osteoinductive effects on DPSCs comparable to that of the healthy individuals. Conclusion: Based on our study, controlled type 2 diabetic patients can be treated similar to their Unaffected peers using 10% PRF exudates as the optimal concentration for DPSCs proliferation and 1% PRF exudates as the optimal concentration for DPSCs osteodifferentiation and formation of mineralized nodules in 7 days.