Possible pitfalls in motherless paternity analysis with related putative fathers

Parentage verification of Valle del Belice dairy sheep using multiplex microsatellite panel

“…The misidentification rates are high compared to other livestock species (Crawford et al, 1993;Laughlin et al, 2003;Weller et al, 2004;Siwek and Knol, 2010), indicating the need to improve the identification system and genealogical data collection. The obtained non-exclusion probabilities and paternity test results indicated that this microsatellite panel is suitable for parentage testing animals from moderately inbred and structured populations, even when subjects are related (De Ungria et al, 2002;von Wurmb-Schwark et al, 2006;Wenk et al, 2006;Scarpetta et al, 2007), and could be used for pedigree reconstruction of Valle del Belice breed.…”

A functional methylome map of ulcerative colitis

“…The twenty monozygotic twins, discordant for ulcerative colitis (screening panel; median age: 25, range 18-70) recruited for this study were tested for mono/dizygosity as previously published (Barbaro and Cormaci 2004;von Wurmb-Schwark et al 2005). The panel for real-time PCR validation of transcript levels consisted of 135 unrelated individuals (validation panel I; n = 30 ulcerative colitis, inflamed; n = 30 ulcerative colitis, noninflamed; n = 30 healthy individuals; n = 15 disease controls, inflamed; n = 30 disease controls, noninflamed; median age: 41, age range 18-76), a subgroup of which was used for pyrosequencing validation of methylation levels (validation panel II; n = 20 ulcerative colitis, inflamed; n = 20 healthy individuals, n = 10 disease controls, inflamed; median age: 41, age range 18-68).…”

Analysis of fortuitous matches in a STR genotype database from Mexico and its forensic efficiency parameters

“…Interpopulation comparisons by Fst distances and Fst p-values are indicated in Additional file 2: Table S2. Mexican-Mestizos from the Western region were closer to Hispanic Americans (probably due to their closer European ancestry) than the remaining Mexican populations, according to the genetic structure previously described in Mexican-Mestizos based on the 13 CODISSTRs (Wurmb-Schwark et al 2006;Rubi-Castellanos et al 2009). AMOVA results suggest significant differentiation between Mexican and American populations (Fst = 1.45%; p = 0.0000), which is approximately seven times larger than that observed among the five Mexican populations studied herein (Fst = 0.198%; p = 0.0000), and 17 times larger than the interpopulation differentiation among the Central Mexican populations (excluding the Western region) (Fst = 0.198%; p = 0.0000).…”

“…Although commercial kits that analyze 15 STRs allow solving most forensic and paternity cases, some pitfalls arise in situations such as the identification of disaster victims, missing persons, and in motherless paternity cases (Wurmb-Schwark et al 2006;Coletti et al 2008;Borovko et al 2009;Li et al 2012;Ziętkiewicz et al 2012). In our experience, the lack of informative reference samples (first-degree relatives) is probably the most common problem when identifying unknown bodies.…”