Possible molecular mechanism underlying cadmium-induced circadian rhythms disruption in zebrafish

Xiao, Bo; Chen, Tian-Ming; Zhong, Yueyang

doi:10.1016/j.bbrc.2016.10.081

Cited by 21 publications

(7 citation statements)

References 23 publications

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“…Thus, the PbCl 2 -treated fish could not sleep normally in the dark cycle and exhibited anxiety-like behavior in the day cycle. The dysregulation of the circadian rhythm due to cadmium poisoning has been reported in zebrafish larvae [46]. The circadian rhythm locomotor activity dysregulation caused by lead overload is the first case reported in adult zebrafish.…”

Section: Discussionmentioning

confidence: 99%

Chronic Exposure to Low Concentration Lead Chloride-Induced Anxiety and Loss of Aggression and Memory in Zebrafish

Thi

Audira

et al. 2020

IJMS

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Lead and lead-derived compounds have been extensively utilized in industry, and their chronic toxicity towards aquatic animals has not been thoroughly addressed at a behavioral level. In this study, we assessed the risk of exposure to lead at a waterborne environmental concentration in adult zebrafish by behavioral and biochemical analyses. Nine tests, including three-dimension (3D) locomotion, novel tank exploration, mirror biting, predator avoidance, social interaction, shoaling, circadian rhythm locomotor activity, color preference, and a short-term memory test, were performed to assess the behavior of adult zebrafish after the exposure to 50 ppb PbCl 2 for one month. The brain tissues were dissected and subjected to biochemical assays to measure the relative expression of stress biomarkers and neurotransmitters to elucidate the underlying mechanisms for behavioral alterations. The results of the behavioral tests showed that chronic exposure to lead could elevate the stress and anxiety levels characterized by elevated freezing and reduced exploratory behaviors. The chronic exposure to PbCl 2 at a low concentration also induced a sharp reduction of aggressiveness and short-term memory. However, no significant change was found in predator avoidance, social interaction, shoaling, or color preference. The biochemical assays showed elevated cortisol and reduced serotonin and melatonin levels in the brain, thus, altering the behavior of the PbCl 2 -exposed zebrafish. In general, this study determined the potential ecotoxicity of long-term lead exposure in adult zebrafish through multiple behavioral assessments. The significant findings were that even at a low concentration, long-term exposure to lead could impair the memory and cause a decrease in the aggressiveness and exploratory activities of zebrafish, which may reduce their survival fitness.(EC10/NOEC-usually more than 14 days) on fish range from 18 to 1559 µg/L and 44 to 437 µg/L, respectively [3,4].More evidence has shown the detrimental impacts of lead on human health, especially at a neurobehavioral level. The neurotoxicity of chronic exposure to low concentrations of lead can impair cognitive performance in childhood through to adulthood [5][6][7][8][9]. Some studies demonstrated the correlation between the exposure to lead at low concentrations and attention-deficit/hyperactivity disorder (ADHD) in children [10]. The development of the central nervous system can be disrupted when children are exposed to low concentrations of lead for a long period of time (blood lead level below 10 mg/dL) [11]. Therefore, lead toxicity has become a serious issue for human health and environmental protection [6]. According to previous studies, the effect of lead on the nervous system includes the disruption of key molecules during neuronal migration and differentiation, interference with synapse formation mediated by a reduction in neuronal sialic acid production and premature differentiation of glial cells, interference with neurotransmitter release, and disruption of the...

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Section: Discussionmentioning

confidence: 99%

Chronic Exposure to Low Concentration Lead Chloride-Induced Anxiety and Loss of Aggression and Memory in Zebrafish

Thi

Audira

et al. 2020

IJMS

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“…The brain transcriptome response of LFP to the light stimulus leads to an upregulation of numerous KEGG pathways (Fig. 1 d), all of which have been linked to the circadian clock [ 29 – 36 ]. In HFP we observe a complete loss of gene activation regarding these KEGG pathways, which we conclude to be the result of the previously mentioned high level of constant neuronal excitation.…”

Section: Discussionmentioning

confidence: 99%

The light response in chickens divergently selected for feather pecking behavior reveals mechanistic insights towards psychiatric disorders

2021

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Background Feather pecking is a serious behavioral disorder in chickens that has a considerable impact on animal welfare and poses an economic burden for poultry farming. To study the underlying genetics of feather pecking animals were divergently selected for feather pecking over 15 generations based on estimated breeding values for the behavior. Methods and results By characterizing the transcriptomes of whole brains isolated from high and low feather pecking chickens in response to light stimulation we discovered a putative dysregulation of micro RNA processing caused by a lack of Dicer1. This results in a prominent downregulation of the GABRB2 gene and other GABA receptor transcripts, which might cause a constant high level of excitation in the brains of high feather pecking chickens. Moreover, our results point towards an increase in immune system-related transcripts that may be caused by higher interferon concentrations due to Dicer1 downregulation. Conclusion Based on our results, we conclude that feather pecking in chickens and schizophrenia in humans have numerous common features. For instance, a Dicer1 dependent disruption of miRNA biogenesis and the lack of GABRB2 expression have been linked to schizophrenia pathogenesis. Furthermore, disturbed circadian rhythms and dysregulation of genes involved in the immune system are common features of both conditions.

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“…Interestingly, visual and light perception upon exposure to Cd were some overrepresented biological functions found on our results that are still hardly discussed in the literature. These pathways might be related either with the role of Cd on circadian disruption through oxidative stress and immune response (Jiménez-Ortega et al, 2011; Xiao et al, 2016), and therefore having an altered perception of the environment (Doria et al, 2018). As well as the differences on Cd damage extent between the dark and the light phase (Xiao et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…These pathways might be related either with the role of Cd on circadian disruption through oxidative stress and immune response (Jiménez-Ortega et al, 2011; Xiao et al, 2016), and therefore having an altered perception of the environment (Doria et al, 2018). As well as the differences on Cd damage extent between the dark and the light phase (Xiao et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Detecting Cd adaptation footprint in C. riparius with a multi-genomic approach

Doria

Hannapel

Pfenninger

2021

Preprint

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Evolutionary processes and acquired tolerance to toxicants are important factors governing how animals respond to chemical exposure. Evidence for increased tolerance to cadmium (Cd), a widely distributed toxic metal in aquatic environments, in Chironomus riparius is conflicting and still questioned if it happens through phenotypic plasticity or genetic adaptation. The present study considered the relevance of directional environmental changes by increasing contaminant concentration in a multigenerational selection experiment. Evaluation of measurable life-cycle traits, transcriptomic responses and quantitative genetics from an evolve and resequencing (E&R) experiment were integrated to assess the potential of C. riparius to adapt to Cd. Survival tests revealed some adaptation to Cd exposure. Genomic analyses showed a strong, genome-wide selective response in all replicates, emphasizing that even control laboratory conditions continually exert selective pressure. The integration of transcriptomic and genomic data could isolate the genes related to Cd acquired resistance. Those genes could be linked to an efflux of metals. Therefore, it is possible to conclude that C. riparius can endure long-term Cd exposure also through genetic adaptation.

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Possible molecular mechanism underlying cadmium-induced circadian rhythms disruption in zebrafish

Cited by 21 publications

References 23 publications

Chronic Exposure to Low Concentration Lead Chloride-Induced Anxiety and Loss of Aggression and Memory in Zebrafish

Chronic Exposure to Low Concentration Lead Chloride-Induced Anxiety and Loss of Aggression and Memory in Zebrafish

The light response in chickens divergently selected for feather pecking behavior reveals mechanistic insights towards psychiatric disorders

Detecting Cd adaptation footprint in C. riparius with a multi-genomic approach

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