Possible mechanism for acceleration of meiotic progression of bovine follicular oocytes by growth factors in vitro

Sakaguchi, Minoru; Dominko, Tanja; Yamauchi, Nobuhiko; Leibfried-Rutledge, ML; Nagai, Takashi; First, N.L.

doi:10.1530/rep.0.1230135

Cited by 43 publications

(31 citation statements)

References 16 publications

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“…The growth factors have been shown to accelerate progression of meiosis [63] and the meiotic cell cycle in bovine oocytes, possibly by increasing H1 and MAP kinase activity during the early stages of maturation [64]. Additionally, the factors secreted by cumulus cells that regulate the disruption of gap junction and cumulus expansion, as shown for pig oocytes [65], may be stimulated by these growth factors [66].…”

Section: Discussionmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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Purpose To investigate the ability of medium conditioned with bovine cumulus-oocyte complexes (COCs) to support nuclear maturation of canine oocytes recovered from domestic dog ovaries. Methods Cumulus-oocyte complexes were obtained from ovaries of domestic bitches (8 months old to 7 years old), and in-vitro maturation was evaluated in TCM-199 supplemented with different concentrations (0, 20, 30 or 50%) of bovine COCs-conditioned medium (BCM). The canine COCs were cultured for 72 or 96 h at 38.5°C in 5% CO 2 , 5% O 2 and 90% N 2 . The bovine COCs-conditioned medium was obtained from culture of bovine COCs with TCM-199 supplemented with 5% FCS for 22 h at 38.5°C in 2% CO 2 , 98% air. Results The proportion of germinal vesicle breakdown (GVBD) after 72 h was significantly higher (P \ 0.05) in medium supplemented with 30% BCM (20.7%) compared with the control group (13.4%). The rates of GVBD-MII stage were significantly higher (P \ 0.05) when oocytes were matured with BCM at concentration of 30% (41.5%) compared with control (26.6%) after 72 h in-vitro culture. After 96 h in-vitro culture, the oocytes matured in medium supplemented with 30% BCM (5.5%) showed a significant increase (P \ 0.05) in the proportion of MII compared with control (0.7%). However, increasing the cultivation time from 72 to 96 h resulted in an increase in oocyte degeneration rate. Conclusions The results suggested that bovine COCsconditioned medium supplementation significantly increased nuclear maturation of canine oocytes.

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Section: Discussionmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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“…Forthermore, IGF-1 stimulates resumption of meiosis and completion of nuclear maturation of porcine oocytes [24]. It has been detected that exposure of bovine oocytes to EGF and IGF-1 accelerated progression of meiosis in COCs [25]. The beneficial effect of IGF-1 on oocyte has been proven in vivo.…”

Section: Discussionmentioning

confidence: 99%

Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress

Zhandi

Towhidi

Nasr-Esfahani

et al. 2009

J Assist Reprod Genet

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Purpose To investigate the effects of Insulin like growth factor-1 (IGF-1) on bovine oocyte developmental competence under heat stress. Methods In Experiment 1, bovine cumulus-oocyte complexes (COCs) were cultured at 38.5 or 41°C for the first 12 h of maturation in the presence of either 100 ng/ml human recombinant (hr)-IGF-1 or acetic acid. In Experiment 2, COCs were cultured in 38.5 or 41°C for the first 12 h of maturation in the presence either of 100 ng/ml hr-IGF-1 or acetic acid. After fertilization, putative zygotes were cultured for 8 days.Results In experiment 1, addition of rh-IGF-1 to maturation medium at 38.5°C significantly increased the proportion of M II oocytes and decreased the percentage of TUNEL-positive oocytes compared to the other groups. However, addition of rh-IGF-1 to maturation medium under heat stress increased the percentage of TUNEL-positive oocytes. In experiment 2, addition of rh-IGF-1 under heat sress did not affect cleavage rate, whereas, blastocyst formation rate decreased in heatstressed and heat-stressed plus rh-IGF-1 groups. Similarly, The number of trophectoderm cells and total cell number were decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups and the percentage of TUNEL-positive nuclei were increased in heat-stressed and heat-stressed plus rh-IGF-1 groups compared to the other groups. Conclusion The results of the present study demonstrate that IGF-1 decreases oocyte developmental competence and total cell number and increases TUNEL-positive nuclei at heat stress condition. These unexpected results of IGF-1 during maturation period under heat stress condition warrant further optimizations and investigations.

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“…In addition to the promotion of GVBD, treatment with EGF also increases first polar body extrusion (20,21) and the number of cells in the blastocyst (22). Because the expression of three EGF-like factors, in a manner similar to BDNF, is increased after the preovulatory LH surge (1), these ovarian paracrine factors could act synergistically to promote first polar body extrusion.…”

Section: Discussionmentioning

confidence: 99%

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

Kawamura

Mulders

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

168

126

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Optimal development of fertilized eggs into preimplantation embryos is essential for reproduction. Although mammalian oocytes ovulated after luteinizing hormone (LH) stimulation can be fertilized and promoted into early embryos in vitro, little is known about ovarian factors important for the conditioning of eggs for early embryo development. Because LH interacts only with ovarian somatic cells, its potential regulation of oocyte functions is presumably mediated by local paracrine factors. We performed DNA microarray analyses of ovarian transcripts and identified brainderived neurotrophic factor (BDNF) secreted by granulosa and cumulus cells as an ovarian factor stimulated by the preovulatory LH surge. Ovarian BDNF acts on TrkB receptors expressed exclusively in oocytes to enhance first polar body extrusion of oocytes and to promote the in vitro development of zygotes into preimplantation embryos. Furthermore, in vivo treatment with a Trk receptor inhibitor suppressed first polar body extrusion and the progression of zygotes into blastocysts. Thus, ovarian BDNF is important to nuclear and cytoplasmic maturation of the oocyte, which is essential for successful oocyte development into preimplantation embryos. Treatment with BDNF could condition the cultured oocytes for optimal progression into the totipotent blastocysts.early embryo development ͉ gonadotropins ͉ ovulation I n vertebrates, rupture of ovarian follicles and final maturation of oocytes occur in response to stimulation by pituitaryderived luteinizing hormones (LH) that act on the somatic granulosa and theca cells surrounding the oocyte. Shortly after stimulation by the preovulatory surge of LH, oocytes arrested at the late prophase resume meiosis characterized by germinal vesicle (nuclear envelope) breakdown (GVBD), chromosome condensation, and extrusion of the first polar body in preparation for fertilization and early embryonic development. Recent studies demonstrated that the endocrine hormone LH stimulates ovarian production of EGF-like factors from granulosa cells and insulin-like 3 from theca cells to promote GVBD (1, 2). In addition to nuclear maturation exemplified by GVBD and extrusion of the first polar body to complete the first meiotic division, oocytes also undergo cytoplasmic maturation characterized by cytoplasmic changes essential for monospermic fertilization, processing of the sperm, and preparation for development to preimplantation embryos (3, 4). Although the spermatozoon provides an essential element for embryo generation, the developmental fate of the embryo is principally dictated by the oocyte. However, few studies have explored ovarian factors that may be important for the conditioning of the oocyte in preparation for fertilization and preimplantation development.Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of proteins known to activate the highaffinity TrkB receptor and the pan-neurotrophin low-affinity receptor p75 (5). Although neurotrophins are widely expressed in the central nervous syst...

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Possible mechanism for acceleration of meiotic progression of bovine follicular oocytes by growth factors in vitro

Cited by 43 publications

References 16 publications

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress

Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos

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