Possible Kefir Biological Effects: 4: Effect of Kefir Beverage on The Histopathological and Macroscopical Changes in Adipose Tissue of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Aref, Abdel-Baset M.; Semmler, Margit; Ahmed, Osama M.; Ünlü, Gülhan; Ali, Lobna Abo; Haridy, Mohie

doi:10.21608/eajbsc.2020.78931

Cited by 2 publications

(2 citation statements)

References 42 publications

(46 reference statements)

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“…The present results show that the total protein and phospholipids materials contents in pancreatic cells of high fat-fed-streptozotocin-induced diabetic male rats which daily treated with kefir (group B) slightly and highly increased respectively than those in group A and group C, this is agreed with that kefir affects keep the bodyweight balanced on high fat-fed-streptozotocin-induced diabetic male rats which fed with a daily kefir gavage dose (Aref et al, 2020).…”

Section: In Experiments Ii: -supporting

confidence: 87%

Possible Short-Term Biological Effects of Kefir Beverage: Ⅰ: Effect of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Pancreas of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Aref

Ünlü

Ahmed

et al. 2021

Egyptian Academic Journal of Biological Sciences, B. Zoology

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Biochemical points of view, the preclinical treatment with kefir or insulin of normal or diabetic male rats, beneficially highly alternates the cellular activities, histochemical and immunohistochemical materials components, and histological architecture. of the pancreas therefore Kefir and insulin may, to some extent, repair the pathological side effects of type 1 diabetes mellitus.The positive results of using kefir and insulin in treating the Pathogen effects of type 1 diabetes mellitus on the pancreas of male mice gave us a hope of possibility to obtain positive clinical applications/implications. Therefore, these positive beneficial results make us continue our work and complete the various pre-clinical trial and clinical experiments phases.

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Section: In Experiments Ii: -supporting

confidence: 87%

Possible Short-Term Biological Effects of Kefir Beverage: Ⅰ: Effect of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Pancreas of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Aref

Ünlü

Ahmed

et al. 2021

Egyptian Academic Journal of Biological Sciences, B. Zoology

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“…The mean relative weight of the liver was calculated in each group at the end of the experiment, P value showed non-significance between the six groups; the values were nearly the same in the untreated and treated rat males, these results agree with Urdaneta et al (2007); Sahin and Yardimci (2009) showed that using kefir supplemented diet had no significant differences the weight of the body organs examined and agree with that kefir has the efficacy to prevent the loss in body weight of diabetic male rats, while neither affects abdominal circumference nor relative fat weight (Aref et. al., 2020).…”

Section: In Experiments IIsupporting

confidence: 78%

Possible Short-Term Biological Effects of Kefir: Ⅱ: Protective Role and Therapeutic Efficacy of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Liver of High-Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Aref

Ünlü

Ahmed

et al. 2021

Egyptian Academic Journal of Biological Sciences, B. Zoology

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This study was designed to investigate the protective role of kefir and insulin consumption on cellular activities, histochemical components, histological architecture, and integrity of the liver by measuring IL-6 and TNFα in high fat-fed STZ-induced diabetic male Wistar rats.From a cell biological and histochemical point of view, both kefir and insulin have beneficial various effects on cellular activities and the histochemical components such as DNA, RNA, total protein, collagen, polysaccharides, and phospholipids materials synthesis in the examined normal and diabetic hepatocytes of the male rats. Biochemically, kefir and insulin have various biochemical effects on the serum IL-6 and TNF-α levels.From a pathological point of view, we can say the beneficial various cell biological and histochemical effects of kefir, pathologically may make kefir, to some extent, completely repair all the pathological side effects of type1 diabetes on the hepatocytes in diabetic rats. Also, insulin repair some of the pathological side effects of diabetes on the hepatocytes.The positive results of using kefir and insulin in treating the pathogen effects of diabetes make it possible to obtain positive clinical applications/implications. Therefore, these positive results encourage us to continue working and complete the various long-term pre-clinical trials and clinical trials phases. Composition of HFD: Induction of Diabetes Mellitus:Diabetes mellitus was experimentally induced in male animals by streptozotocin, STZ was dissolved in cold 0.01 M citrate buffer, pH 4.5, and always prepared freshly for immediate use within 5 minutes. Rats were fasted for overnight to induce diabetes by intraperitoneal (IP) injection of streptozotocin (STZ) at the dose of 45 mg/kg body weight (Judiono et al., 2011;Suharyo et al., 2012;Giovana et al., 2014). The normal control group was given citrate buffer without STZ. The development of diabetes was confirmed after 48 hours -7 days of STZ injection. The animals with fasting blood glucose levels of more than 200 mg/dl were considered as diabetic and included in this study. For glucose assay, blood samples were collected from the tail-tip or tail-vein of the rats and measured using a glucometer. Animal Grouping:The experimental animals were divided into six groups 10 animals each, 3 nondiabetic, and 3 diabetic groups: Experiment I: Group 1: Control animals, (negative group) were fed a standard diet plus oral administration of distilled water at a dose of 0.7 ml/animal/day. Group 2: Animals were fed a standard diet and received oral administration of kefir (0.7 ml/animal/day). Group 3: Animals received a high-fat diet (HFD) and additionally oral administration of kefir (0.7 ml/animal/day). Experiment II: Group A: Diabetic group (positive group), was fed HFD and received oral administration of distilled water (0.7 ml/animal/day). Group B: Diabetic animals received HFD plus oral administration of kefir (0.7 ml/animal/day. Group C: Diabetic group, fed HFD, was subcutaneously injected insulin (0.76 UI/200 m...

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Possible Kefir Biological Effects: 4: Effect of Kefir Beverage on The Histopathological and Macroscopical Changes in Adipose Tissue of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Cited by 2 publications

References 42 publications

Possible Short-Term Biological Effects of Kefir Beverage: Ⅰ: Effect of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Pancreas of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Possible Short-Term Biological Effects of Kefir Beverage: Ⅰ: Effect of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Pancreas of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Possible Short-Term Biological Effects of Kefir: Ⅱ: Protective Role and Therapeutic Efficacy of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Liver of High-Fat-Fed STZ- Induced Diabetic Male Wistar Rat

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