Possible involvement of tetrahydrobiopterin in the disturbance of redox homeostasis in sepsis – Induced brain dysfunction

Gamal, Maha; Moawad, Jackline; Rashed, Laila Ahmed; Morcos, Mary Attia; Sharawy, Nivin

doi:10.1016/j.brainres.2018.02.008

Cited by 20 publications

(17 citation statements)

References 76 publications

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“…Malonaldehyde (MDA) was used for the estimation of damage by reactive oxygen species (H 2 O 2 ). The level of MDA was measured spectrophotometrically at 535 nm using the thiobarbituric acid reactive substance (TBARS) Assay Kit (Biodiagnostic; Egypt) according to the method described by Gamal et al (2018) .…”

Section: Methodsmentioning

confidence: 99%

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Combined Oral and Topical Application of Pumpkin (Cucurbita pepo L.) Alleviates Contact Dermatitis Associated With Depression Through Downregulation Pro-Inflammatory Cytokines

et al. 2021

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Background: Depression and contact dermatitis (CD) are considered relatively common health problems that are linked with psychological stress. The antioxidant, anti-inflammatory, and antidepressant activities of pumpkin were previously reported.Objectives: This study aimed to evaluate the efficacy of the combined topical and oral application of pumpkin fruit (Cucurbita pepo L.) extract (PE) in relieving CD associated with chronic stress–induced depression and compare it to the topical pumpkin extract alone and to the standard treatment.Materials and Methods: Forty male albino rats were exposed to chronic unpredictable mild stress (CUMS) for 4 weeks for induction of depression and then exposed to (1-fluoro-2, 4-dinitrofluorobenzene, DNFB) for 2 weeks for induction of CD. Those rats were assigned into 4 groups (n = 10 each); untreated, betamethasone-treated, PE-treated and pumpkin extract cream, and oral-treated groups. Treatments were continued for 2 weeks. All groups were compared to the negative control group (n = 10). Depression was behaviorally and biochemically confirmed. Serum and mRNA levels of pro-inflammatory cytokines, such as TNF-α, IL-6, COX-2, and iNOS, were assessed. Oxidant/antioxidant profile was assessed in the serum and skin. Histopathological and immunohistochemical assessments of affected skin samples were performed.Results: Pumpkin extract, used in this study, included a large amount of oleic acid (about 56%). The combined topical and oral administration of PE significantly reduced inflammatory and oxidative changes induced by CD and depression compared to the CD standard treatment and to the topical PE alone. PE significantly alleviated CD signs and the histopathological score (p < 0.001) mostly through the downregulation of pro-inflammatory cytokines and the upregulation of antioxidants.Conclusion: Pumpkin extract, applied topically and orally, could be an alternative and/or complementary approach for treating contact dermatitis associated with depression. Further studies on volunteer patients of contact dermatitis are recommended.

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Section: Methodsmentioning

confidence: 99%

“…To quantify catalase (CAT) activity, a calibration curve was generated for the assay using kits (Biodiagnostic; Egypt). The method used was described by Gamal et al (2018) .…”

Section: Methodsmentioning

confidence: 99%

Combined Oral and Topical Application of Pumpkin (Cucurbita pepo L.) Alleviates Contact Dermatitis Associated With Depression Through Downregulation Pro-Inflammatory Cytokines

et al. 2021

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“…To assess the antioxidant effect of PE, the levels of SOD (Biodiagnostic; Egypt), GPX (Randox Labs, Crumlin, UK) and CAT (Biodiagnostic; Egypt) were measured in serum according to the previously described methods [19].…”

Section: Assessment Of Superoxide Dismutase (Sod) Glutathione Peroximentioning

confidence: 99%

“…Assessment of malonaldehyde [18] in the serum: Malonaldehyde (Biodiagnostic; Egypt) was measured for the estimation of damage by reactive oxygen species (H2O2) according to the method described by [19].…”

Section: Assessment Of Behavioral Changesmentioning

confidence: 99%

Brain-Derived Neurotropic Factor (BDNF) Mediates the Protective Effect of L.Cucurbita Pepo on Salivary Glands of Depressed Rat Evident by Structural, Biochemical and Molecular Study

Hm¹,

Ea²,

Zm³

et al. 2021

Preprint

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BackgroundAcute and chronic stresses affect the salivary glands which are the source of plasma BDNF during stressful conditions. Pumpkin is a medicinal plant with an evident antioxidant, anti-inflammatory and potential antidepressant effects. This work was conducted to assess the impact of chronic unpredictable mild stress (CUMS) on the structure of albino rats’ salivary glands and evaluate the role of pumpkin extract (Pump) in ameliorating this effect. MethodsFour groups (n=10 each) of male albino rats included in this study; the control, CUMS, CUMS+ﬂuoxetine and (CUMS+Pump). Corticosterone, pro-inflammatory cytokines (TNF-α & IL-6) and the oxidant/antioxidant profile were all assessed in the serum. BDNF mRNA level was measured in the salivary glands using qRT-PCR. Histopathological changes of the salivary glands were also assessed. ResultsDepression was confirmed behaviorally and biochemically. Exposure to CUMS significantly (p< 0.001) up-regulated the level of serum corticosterone. CUMS induced degenerative changes in the secretory and ductal system, atrophy of acini and increased apoptosis of the acinar and ductal cells. Both fluoxetine and Pump significantly up-regulated (p<0.001) BDNF expression in the salivary glands and ameliorated the CUMS-induced histopathological alterations in the salivary glands. Pumpkin significantly (p<0.001) increased the serum levels of antioxidant enzymes SOD, GPX and CAT and reduced the serum levels of the pro-inflammatory cytokines TNF-α, IL-6.ConclusionPumpkin ameliorates chronic stress-induced depression in rats by exerting a promising anti-inflammatory, antioxidant and anti-depressant-like effects in rats exposed to CUMS. Pump subsequently improved stress-induced structural changes in the salivary glands that might be due to the glands up-regulation of BDNF expression.Trial registrationNot applicable.

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“…To quantify the catalase (CAT) activity, a calibration curve was generated for the assay and all samples, using Biodiagnostic; Egypt assay kits. The method described by Gamal et al [ 19 ] Glutathione peroxidase (GPx) kit (Randox Labs, Crumlin, UK) was used where GPx catalyzes the oxidation of glutathione with cumene hydroperoxide. The method described by Gamal et al was used.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

Efficacy of sweet pumpkin in relieving contact dermatitis in chronically stressed rats

Huwait

2020

J Microsc Ultrastruct

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Background: Contact dermatitis (CD) is considered among the common inflammatory skin diseases worldwide. Cucurbita moschata Duchesne has antioxidant, anti-inflammatory, and antidepressant activity beside many other beneficial effects. Objectives: This study aimed to assess the effect of pumpkin fruit extract in treating CD in mice exposed to chronic stress and to explore the mechanism through which pumpkin can relief these changes. Materials and Methods: Thirty male albino rats were divided into three groups ( n = 10); the control and two experimental groups that were exposure to chronic unpredictable mild stress for 4 weeks then painting with 1-fluoro-2,4-dinitrofluorobenzene (DNFB) for 3 consecutive days/week for 2 weeks to induce CD. Biochemical assessment of corticosterone level and antioxidants activity was performed. Skin of affected areas was excised, processed for histopathological examination. Results: DNFB-induced CD presented with dryness, hardness, and scaling. There was a significant reduction ( P < 0.001) in the levels of superoxide dismutase, glutathione peroxidase and catalase activity in the skin of rats had CD. Histopathologically, the shin showed hyperplastic-thickened epidermis, focal elongation of the rete ridges, inflammatory cells infiltration in the superficial dermis, and increased collagen fibers. Local administration of pumpkin extract significantly increased the antioxidants activity in the skin and alleviated the CD-associated changes. Conclusions: This study showed that the pumpkin fruit extract could have a potential in treating CD in stressed conditions mainly via its enhancement of skin antioxidant activity.

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Possible involvement of tetrahydrobiopterin in the disturbance of redox homeostasis in sepsis – Induced brain dysfunction

Cited by 20 publications

References 76 publications

Combined Oral and Topical Application of Pumpkin (Cucurbita pepo L.) Alleviates Contact Dermatitis Associated With Depression Through Downregulation Pro-Inflammatory Cytokines

Combined Oral and Topical Application of Pumpkin (Cucurbita pepo L.) Alleviates Contact Dermatitis Associated With Depression Through Downregulation Pro-Inflammatory Cytokines

Brain-Derived Neurotropic Factor (BDNF) Mediates the Protective Effect of L.Cucurbita Pepo on Salivary Glands of Depressed Rat Evident by Structural, Biochemical and Molecular Study

Efficacy of sweet pumpkin in relieving contact dermatitis in chronically stressed rats

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