Possible involvement of lactate in neuroglial interaction through nicotinic cholinergic synapses in the cranial cervical sympathetic ganglion

Abstract

Activities of LDH and its H- and M-isoforms in neurons and satellite gliocytes of the cranial cervical sympathetic ganglion in rabbits under normal conditions and during nicotinic cholinergic synapse blockade were evaluated by integral cytophotometry in tissue sections. Normally activity of H-isoform predominates in neurons and M-isoform in satellite gliocytes. Blockade of the cranial cervical sympathetic ganglion significantly decreased LDH activity (H- and M-isoforms) in neurons in direct proportion to the number of blocked nicotinic cholinergic receptors. Activity of M-isoform in satellite gliocytes decreased with increasing the degree of blockade, while activity of H-isoform did not change. The isoenzyme profile of LDH in satellite gliocytes reached the level of intact neurons. Presumably, lactate production in satellite gliocytes is regulated by sympathetic neurons through nicotinic cholinergic synapses.

Expression of Cd38 and Сх43 in the Midbrain Cells at Experimental Parkinson's Disease

“…Эта гипотеза носит название «лактат-челночной» (astrocyte-neuron lactate shuttle hypothesis) предполагая, что перенос глутамата астроцитами после активации нейронов стимулирует астроцитарный гликолиз и продукцию лактата. Лактат, в свою очередь, покидает астроциты и используется нейронами как источник энергии [1,5].…”