Possible Increased Pathogenicity of Pandemic (H1N1) 2009 Influenza Virus upon Reassortment

Schrauwen, Eefje J. A.; Herfst, Sander; Chutinimitkul, Salin; Bestebroer, Theo M.; Rimmelzwaan, Guus F.; Osterhaus, Albert D. M. E.; Kuiken, Thijs; Fouchier, Ron A. M.

doi:10.3201/eid1702.101268

Cited by 69 publications

(67 citation statements)

References 30 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This clade VII was represented by maximum number of isolates from geographically diverse areas. The tree topology was found similar as discussed earlier [19]. The clade VII, which is the largest clade is represented by H1N1pdm virus isolated from Japan, Mexico, China, Asia and several states of the USA.…”

Section: Phylogenetic Analysissupporting

confidence: 72%

“…With the number of reported pandemic cases of H1N1 virus in many parts of the world and continued viral persistence with elevated activity in India and nearby countries (Nepal, Sri Lanka, Bangladesh), has given an urgent need to track the global dispersion of this virus in humans. [18,19] but there was moderate number of H1N1pdm cases in 2012-2013. In this study, one Indian isolate with travel history confirmed by virus specific CPE, HA as well as CDC Real time RT-PCR was selected for complete HA gene characterization.…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Laboratory Investigation and Molecular Epidemiology of H1N1pdm Virus 2012-2013 from India

Sharma¹,

Joshi²,

K³

et al. 2015

J Phylogenetics Evol Biol

View full text Add to dashboard Cite

Influenza A virus is the most unpredictable global threat, past pandemic witnessed the community swaps happened due to this virus. The recent emergence of H7N9 in China is the result of new and recent reassortmant of Influenza A virus. Emergence of a novel human influenza A virus (H1N1pdm) in 2009 is an unprecedent event of molecular virology. This novel emerged virus affected millions of persons and raised WHO pandemic alert to phase 6. Therefore continuous monitoring is required to have adequate genome information and to track mutations during course of viral evolution. In present study we investigated acute phase suspected In the course of its evolution, 2009-2013 the virus acquired many mutations and which could be responsible for severity of this virus and emergence of new virus subtypes. India also witnessed high morbidity and mortality reported from all parts of the country. This study clearly indicates that recently reported cosmopolitan clade VII has undergone positive selection in India as well globally revealed continuous circulation of clade VII since 2009. This study of recent H1N1pdm Indian virus isolate will facilitate future epidemiological surveillance in Indian subcontinent and abroad.

show abstract

Section: Phylogenetic Analysissupporting

confidence: 72%

Section: Discussionmentioning

confidence: 96%

Laboratory Investigation and Molecular Epidemiology of H1N1pdm Virus 2012-2013 from India

Sharma¹,

Joshi²,

K³

et al. 2015

J Phylogenetics Evol Biol

View full text Add to dashboard Cite

show abstract

“…We first generated reassortant viruses by cotransfecting 293T cells with two complete sets of reverse genetics plasmids encoding the viral RNAs of MO and EN viruses as previously described (4,29). The supernatants of plasmid-transfected cells then were used for plaque assays on MDCK cells.…”

Section: Resultsmentioning

confidence: 99%

Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses

Essere

Yver

Gavazzi

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The fragmented nature of the influenza A genome allows the exchange of gene segments when two or more influenza viruses infect the same cell, but little is known about the rules underlying this process. Here, we studied genetic reassortment between the A/Moscow/10/99 (H3N2, MO) virus originally isolated from human and the avian A/Finch/England/2051/91 (H5N2, EN) virus and found that this process is strongly biased. Importantly, the avian HA segment never entered the MO genetic background alone but always was accompanied by the avian PA and M fragments. Introduction of the 5′ and 3′ packaging sequences of HA MO into an otherwise HA EN backbone allowed efficient incorporation of the chimerical viral RNA (vRNA) into the MO genetic background. Furthermore, forcing the incorporation of the avian M segment or introducing five silent mutations into the human M segment was sufficient to drive coincorporation of the avian HA segment into the MO genetic background. These silent mutations also strongly affected the genotype of reassortant viruses. Taken together, our results indicate that packaging signals are crucial for genetic reassortment and that suboptimal compatibility between the vRNA packaging signals, which are detected only when vRNAs compete for packaging, limit this process.hemagglutinin | RNA packaging

show abstract

“…Although the NA is grouped within the human-like H3N2 influenza lineage, North American triple reassortant SIVs containing a similar NA gene have been circulating in US swine herds for more than 5 years. Nev-ertheless, continuous circulation of pH1N1 in swine will increase the chance of further reassortment with human, avian or swine influenza viruses [9,17,22] and could result in a novel virus with the potential to cause infection and efficient transmission among humans. In conclusion, the emergence of novel reassortant H3N2 SIVs in US swine is further evidence of reassortment between pH1N1 and endemic SIVs.…”

mentioning

confidence: 99%

Emergence of novel reassortant H3N2 swine influenza viruses with the 2009 pandemic H1N1 genes in the United States

Liu

et al. 2011

Arch Virol

View full text Add to dashboard Cite

Possible Increased Pathogenicity of Pandemic (H1N1) 2009 Influenza Virus upon Reassortment

Cited by 69 publications

References 30 publications

Laboratory Investigation and Molecular Epidemiology of H1N1pdm Virus 2012-2013 from India

Laboratory Investigation and Molecular Epidemiology of H1N1pdm Virus 2012-2013 from India

Critical role of segment-specific packaging signals in genetic reassortment of influenza A viruses

Emergence of novel reassortant H3N2 swine influenza viruses with the 2009 pandemic H1N1 genes in the United States

Contact Info

Product

Resources

About