Possible Crosstalk of the Immune Cells within the Lung and Mediastinal Fat-Associated Lymphoid Clusters in the Acute Inflammatory Lung Asthma-Like Mouse Model

Elewa, Yaser Hosny Ali; Elwakil, Mahmoud M. Abd; Ichii, Osamu; Nakamura, Teppei; Mohamed, Sherif K.; Kon, Yasuhiro

doi:10.3390/ijms22136878

Cited by 3 publications

(1 citation statement)

References 31 publications

(63 reference statements)

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“…Interestingly, our previous studies have shown that the inflammation induce the development of MFALCs in comparison to the control group in healthy and autoimmune disease mouse strains. Additionally, we revealed close correlation between the development of MFALCs and lung injury (Elewa et al, 2016(Elewa et al, , 2018(Elewa et al, , 2021a. Similarly, our study revealed the existence of significant positive correlations between MFALCs size, and the degree of lung injury, as well as between the immune cells (macrophages, B-and T-lymphocytes) within MFALCs and lung infiltration.…”

Section: Discussionsupporting

confidence: 80%

Histopathological Impact of Bleomycin on Lung Injury and Development of Mediastinal Fat-Associated Lymphoid Clusters in the Lymphoproliferative Mouse Model

Elewa

Ichii

Mohamed

et al. 2022

Microscopy and Microanalysis

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The purpose of this study is to elucidate the impact of bleomycin on the degree of lung injury and development of mediastinal fat-associated lymphoid clusters (MFALCs) in the lymphoproliferative mouse model (MRL/MpJ-Faslpr/lpr “Lpr”) and its control strain (MRL/MpJ “MpJ”). We analyzed immune cells, the degree of proliferation, lymphatic vessels (LVs), and high endothelial venules (HEVs) in lungs and MFALCs in Lpr and MpJ mice on the 7th and 21st days following intranasal instillation of either bleomycin (BLM group) or PBS (PBS group). The BLM group showed a significant increase in the size of MFALCs, lung injury score, and positive area ratios of LVs, HEVs, and immune cells (especially macrophages, B- and T-lymphocytes) on both days 7 and 21. Interestingly, the lungs in the BLM group on day 21 showed higher collagen deposition and cellular infiltration in MpJ and Lpr, respectively. Moreover, significant positive correlations were observed between the size of MFALCs and lung injury. In conclusion, BLM could exert lung fibrosis or lymphoproliferative infiltration in chronic stages in MpJ and Lpr, respectively, and this varied effect could be due to the variations in the degree of immune cell proliferation and the development of LVs and HEVs among the studied strains.

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Section: Discussionsupporting

confidence: 80%

Histopathological Impact of Bleomycin on Lung Injury and Development of Mediastinal Fat-Associated Lymphoid Clusters in the Lymphoproliferative Mouse Model

Elewa

Ichii

Mohamed

et al. 2022

Microscopy and Microanalysis

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Impact of intravenous/intranasal polyinosinic–polycytidylic acid administration on the mediastinal fat-associated lymphoid clusters and lung tissue in healthy mice

Elewa,

Khalifa,

Zahran

2023

Annals of Anatomy - Anatomischer Anzeiger

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Microenvironmental Changes in Mediastinal Fat-associated Lymphoid Clusters and Lungs in Early and Late Stages of Metastatic Lung Cancer Induction

Elewa

Elwakil

Harashima

et al. 2023

Microscopy and Microanalysis

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The prognosis of metastatic lung melanoma (MLM) has been reported to be poor. An increasing number of studies have reported the function of several immune cells in cancer regression. Although the function of mediastinal fat-associated lymphoid clusters (MFALCs) in the progression of inflammatory lung lesions has been previously reported, the association between MLM progression and MFALCs development has remained unexplored. Herein, we compared the microenvironmental changes in the lungs and MFALCs among phosphate-buffered saline (PBS) and cancer groups at early (1 week) and late (2 weeks) stages following the intravenous injection of B16-F10 melanoma cells into C57BL/6 mice. Except for lung CD4+ helper T-cells and Iba1+ macrophage populations of early stage, we observed a significant increase in the proliferating and immune cell (CD20+ B-lymphocytes, CD3+ T-lymphocytes, CD8+ cytotoxic T-cells, CD16+ natural killer (NK) cells populations, area of high endothelial venules, and lung lymphatic vessels in cancer groups at both the stages as compared with the PBS groups. Furthermore, a significant positive correlation was observed between immune cell populations in MFALCs and the lungs (B- and T-lymphocytes, and NK cells in both stages). Collectively, our findings suggest a promising cancer therapeutic strategy via targeting immune cells in MFALCs.

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Possible Crosstalk of the Immune Cells within the Lung and Mediastinal Fat-Associated Lymphoid Clusters in the Acute Inflammatory Lung Asthma-Like Mouse Model

Cited by 3 publications

References 31 publications

Histopathological Impact of Bleomycin on Lung Injury and Development of Mediastinal Fat-Associated Lymphoid Clusters in the Lymphoproliferative Mouse Model

Histopathological Impact of Bleomycin on Lung Injury and Development of Mediastinal Fat-Associated Lymphoid Clusters in the Lymphoproliferative Mouse Model

Impact of intravenous/intranasal polyinosinic–polycytidylic acid administration on the mediastinal fat-associated lymphoid clusters and lung tissue in healthy mice

Microenvironmental Changes in Mediastinal Fat-associated Lymphoid Clusters and Lungs in Early and Late Stages of Metastatic Lung Cancer Induction

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