Possible Association of Single Nucleotide Polymorphism in Titin Gene with Growth-Related Trait in Japanese Black Beef Cattle

Yamada, Takahisa; Sasaki, Seiki; Sukegawa, Shin; Takahagi, Youichi; Morita, Mitsuo; Murakami, Hiroshi; Morimatsu, Fumiki; Fujita, Tatsuo

doi:10.3923/javaa.2011.2603.2606

Cited by 5 publications

(6 citation statements)

References 2 publications

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“…Further, it has been reported that the EEF1A2 (Serão et al 2011) and TTN (Davoli et al 2011) exhibited higher expression levels in skeletal muscle of pig breed with higher growth rate than in one with lower growth rate, consistent with our within-breed study using Landrace pig. Besides, our previous study showed that a polymorphism in the TTN was associated with growth-related trait in bovine association study using Japanese Black beef cattle population (Yamada et al 2011). Based on these results, we suggested that the upregulated EEF1A2, TSG101 and TTN genes corresponded to the intriguing genes showing differential expression profiles associated with growth rate in skeletal muscle tissue of Landrace weanling pig.…”

Section: Discussionmentioning

confidence: 71%

Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig

Komatsu

Sukegawa²,

Yamashita

et al. 2016

J Genet

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Suppression subtractive hybridization was used to identify genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig. Two subtracted cDNA populations were generated from musculus longissimus muscle tissues of selected pigs with extreme expected breeding values at the age of 100 kg. Three upregulated genes (EEF1A2, TSG101 and TTN) and six downregulated genes (ATP5B, ATP5C1, COQ3, HADHA, MYH1 and MYH7) in pig with genetic propensity for higher growth rate were identified by sequence analysis of 12 differentially expressed clones selected by differential screening following the generation of the subtracted cDNA population. Real-time PCR analysis confirmed difference in expression profiles of the identified genes in musculus longissimus muscle tissues between the two Landrace weanling pig groups with divergent genetic propensity for growth rate. Further, differential expression of the identified genes except for the TTN was validated by Western blot analysis. Additionally, the eight genes other than the ATP5C1 colocalized with the same chromosomal positions as QTLs that have been previously identified for growth rate traits. Finally, the changes of expression predicted from gene function suggested association of upregulation of expression of the EEF1A2, TSG101 and TTN genes and downregulation of the ATP5B, ATP5C1, COQ3, HADHA, MYH1 and MYH7 gene expression with increased growth rate. The identified genes will provide an important insight in understanding the molecular mechanism underlying growth rate in Landrace pig breed.

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Section: Discussionmentioning

confidence: 71%

Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig

Komatsu

Sukegawa²,

Yamashita

et al. 2016

J Genet

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“…We speculate that the c.*188G>A SNP might affect mRNA stability to decrease the expression level of AKIRIN2, resulting in high growth performance in QC. The g.231054C>T SNP in the promoter region of TTN showed associations with marbling score and longissimus muscle area in JB (Yamada et al, 2009a(Yamada et al, , 2011. Since TTN is known to be involved in myofibrillogenesis (Itoh-Satoh et al, 2002), we can hypothesize that the g.231054C>T SNP in the promoter region might have an impact on TTN expression and also growth performance by affecting TTN promoter activity in QC.…”

Section: Discussionmentioning

confidence: 92%

“…Thus, these genes could be considered as important candidate genes for carcass and growth traits in beef cattle. Furthermore, the c.*188G>A single nucleotide polymorphism (SNP) in the 3' untranslated region (UTR) of AKIRIN2, the g.231054C>T SNP in the promoter region of TTN, the g.1471620G>T SNP in the promoter region of EDG1 and the g.70014208A>G SNP in the promoter region of MYBPC1, showed associations of these SNPs with growth or carcass traits in JB (Sasaki et al, 2009;Yamada et al, 2009 a, b;Yamada et al, 2011;Tong et al, 2014aTong et al, , 2015 and Korean native (KN) (Kim et al, 2013) cattle. However, the relationships of these SNPs with growth and carcass traits in Chinese cattle breed have not been investigated.…”

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confidence: 99%

Association of SNPs in AKIRIN2, TTN, EDG1 and MYBPC1 Genes with Growth and Carcass Traits in Qinchuan Cattle

Cheng

Sheng

et al. 2022

Annals of Animal Science

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Growth and carcass traits are the main breeding objectives in beef cattle. The aim of this study was to confirm genetic effects of the c.*188G>A SNP of AKIRIN2, the g.231054C>T SNP of TTN, the g.1471620G>T SNP of EDG1 and the g.70014208A>G SNP of MYBPC1 on growth and carcass traits in Chinese Qinchuan (QC) cattle, as well as to compare the frequencies of the well-characterized alleles of these SNPs among six Chinese cattle populations, three Japanese cattle populations, two European cattle populations and one Korean cattle population. In this study, a total of 665 cattle samples were genotyped using MassARRAY and PCR-RFLP. Association analysis explored effects of four SNPs on growth and carcass traits including body length, wither height, hip height, hip width, rump length, chest depth, chest circumference, back fat thickness, ultrasound longissimus muscle area and ultrasound longissimus muscle depth in QC (P<0.05 to P<0.001). The well-characterized A (c.*188G>A), T (g.231054C>T) and T (g.1471620G>T) alleles in Japanese Black cattle were significantly higher than Chinese cattle breeds, on the contrary, the G allele (g.70014208A>G) was markedly higher in Chinese cattle breeds than other cattle breeds. These results suggest that the four SNPs might be useful as a molecular marker for growth-related traits in Chinese QC cattle.

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“…The c.*188G > A SNP of AKIRIN2 showed associations with marbling score in JB [13] as well as marbling score and longissimus muscle area in KN [19]. The g.231054C > T SNP of TTN showed associations with marbling score and longissimus muscle area in JB [14,15]. The g.1471620G > T SNP of EDG1 showed association with marbling score in JB [16], simultaneously one other SNP in EDG1 had effects on marbling score, subcutaneous fat thickness, carcass weight, longissimus muscle area and rib thickness [29,30].…”

Section: Discussionmentioning

confidence: 92%

“…Thus, these genes could be considered as important candidate genes for growth-related traits in beef cattle. Furthermore, the c.*188G > A single nucleotide polymorphism (SNP) in AKIRIN2, the g.231054C > T SNP in TTN, the g.1471620G > T SNP in EDG1, and the g.70014208A > G SNP in the MYBPC1 genes showed associations of these SNPs with growth-related traits in JB [13][14][15][16][17][18] and Korean native (KN) [19] cattle. However, the relationships of these SNPs with growth-related traits in Chinese cattle breed have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

SNPs in AKIRIN2, TTN, EDG1 and MYBPC1 genes are associated with growth-related traits in Chinese Qinchuan cattle

Chen

Zhao

et al. 2020

Preprint

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Growth performances are the main breeding objectives in Chinese beef cattle. The objective of this study was to confirm genetic effects of the c.*188G > A SNP in AKIRIN2, the g.231054C > T SNP in TTN, the g.1471620G > T SNP in EDG1, and the g.70014208A > G SNP in MYBPC1 gene on growth-related traits in Chinese Qinchuan cattle, as well as to compare the frequencies of the well-characterized alleles of these SNPs among six Chinese cattle populations, three Japanese cattle populations, two European cattle populations and one Korean cattle breed. In this study, a total of 655 cattle samples were genotyped using MassARRAY and PCR-RFLP. Association analysis indicated that the four SNPs have effects one to six indexes of growth-related traits including body length, wither height, hip height, hip width, rump length, chest depth and chest circumference in Chinese Qinchuan cattle (P < 0.05 to P < 0.001). The well-characterized A (c.*188G > A), T (g.231054C > T) and T (g.1471620G > T) alleles in Japanese Black cattle were significantly higher than Chinese cattle breeds, on the contrary, the G allele (g.70014208A > G) was markedly higher in Chinese cattle breeds than other cattle breeds. These results suggest that the four SNPs might be useful as a molecular marker for growth-related traits in Chinese Qinchuan cattle.

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Possible Association of Single Nucleotide Polymorphism in Titin Gene with Growth-Related Trait in Japanese Black Beef Cattle

Cited by 5 publications

References 2 publications

Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig

Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig

Association of SNPs in AKIRIN2, TTN, EDG1 and MYBPC1 Genes with Growth and Carcass Traits in Qinchuan Cattle

SNPs in AKIRIN2, TTN, EDG1 and MYBPC1 genes are associated with growth-related traits in Chinese Qinchuan cattle

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