Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy

Abstract

Vδ T cells in AILs are cytotoxic to tumour cells in ascites and may be considered in adoptive immunotherapy.

Application of gamma-delta T cells obtained by ascites filtration for immunotherapy against malignant refractory ascites

“…In this study, the ratio Vδ2 + T cells in CD3 + cells in the ascitic fluid obtained from a CART filter was 1.2%, which was similar to the ratio of cells obtained from the peripheral blood. Reportedly, the growth rate of γδ T cells in the ascitic fluid of patients with ovarian cancer who underwent direct paracentesis with 2M3B1-PP and IL2 was 1.9 times and 3.8 times with Zol and IL2 8) ,…”

“…It is reported that the ratio of Vδ2 + T cells in CD3 + cells of PBMC in 24 patients with ovarian cancer, fallopian tube cancer and peritoneal carcinoma were 1.42% 8) . In this study, the ratio Vδ2 + T cells in CD3 + cells in the ascitic fluid obtained from a CART filter was 1.2%, which was similar to the ratio of cells obtained from the peripheral blood.…”

“…which is similar to the growth rate we obtained from a CART filter. Compared with peripheral blood γδ T cells, the proliferation potency of γδ T cells was remarkably impaired in the ascitic fluid 8) . The growth rates of cells cultured from ovarian cancer patients' PBMCs with 2M3B1-PP, IL2 and IL18 were reported to significantly increase than those with IL2 alone 17) .…”

“…It was reported that the culture of γδ T cells is possible in the ascitic fluid 8) . Initially, we collected AILs from the hollow fiber of the filtration column used at discarding ascitic filtration concentration.…”

Weighing in on weight loss in pancreatic cancer

“…Cachexia in humans can be exacerbated if deficiencies occur in essential nutrients 7,8 , for example long-chain fatty acids and vitamin D, whose uptake is facilitated by pancreatic enzymes such as lipase. The administration of fatty acids increases skeletal-muscle mass in people with pancreatic cancer, particularly when this supplementation is combined with pancreatic enzymes 9 .…”