Positively-charged hierarchical PEDOT interface with enhanced electrode kinetics for NADH-based biosensors

Meng, Lingyin; Turner, Anthony; Mak, Wing Cheung

doi:10.1016/j.bios.2018.08.017

Cited by 30 publications

(19 citation statements)

References 60 publications

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“…These proved that EDOT was successfully polymerized to produce PEDOT through a-a' coupling. [16] Furthermore,the full XPS spectrum investigated the chemical composition of SiC@Au-PEDOT NWs (Figure 1g,hand Figure S2). Specifically,S2p spectrum (Figure 1g)s howed the characteristic peaks of spin-split doublet S2 pa t1 64.9 eV (S 2p 1/2 )a nd 163.7 eV (S 2p 3/2 ) originated from Sa toms in PEDOT.…”

Section: Angewandte Chemiementioning

confidence: 99%

Large‐Scale Synthesis of Functionalized Nanowires to Construct Nanoelectrodes for Intracellular Sensing

Jiang

et al. 2021

Angew Chem Int Ed

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A strategy for one‐pot and large‐scale synthesis of functionalized core–shell nanowires (NWs) to high‐efficiently construct single nanowire electrodes is proposed. Based on the polymerization reaction between 3,4‐ethylenedioxythiophene (EDOT) and noble metal cations, manifold noble metal nanoparticles‐polyEDOT (PEDOT) nanocomposites can be uniformly modified on the surface of any nonconductive NWs. This provides a facile and versatile approach to produce massive number of core–shell NWs with excellent conductivity, adjustable size, and well‐designed properties. Nanoelectrodes manufactured with such core–shell NWs exhibit excellent electrochemical performance and mechanical stability as well as favorable antifouling properties, which are demonstrated by in situ intracellular monitoring of biological molecules (nitric oxide) and unraveling its relevant unclear signaling pathway inside single living cells.

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Section: Angewandte Chemiementioning

confidence: 99%

Large‐Scale Synthesis of Functionalized Nanowires to Construct Nanoelectrodes for Intracellular Sensing

Jiang

et al. 2021

Angew Chem Int Ed

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“…Taken together, these data strongly suggest that cell viability decreased with an increasing PHMG-p concentration and exposure time, and that our EC assay has the potential to not only replace the conventional cell viability assay, but also to aid in cytotoxicity studies of a variety of toxic materials as continuous cell viability monitoring is also possible. Table 1 presents the many types of sensors to measure NADH used in the past 5 years [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. Our electrocatalytic sensor has the advantages of comprising one simple step, consuming a low volume of sample (50 μL), and having a low LOD.…”

Section: Resultsmentioning

confidence: 99%

Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells

et al. 2022

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Nicotinamide adenine dinucleotide (NADH) is an important cofactor involved in metabolic redox reactions in living cells. The detection of NADH in living animal cells is a challenge. We developed a one-step monitoring method for NADH via an electrocatalytic reaction that uses a surface-modified, screen-printed electrode (SPE) having a redox active monolayer 4′-mercapto-N-phenlyquinone diamine (NPQD) formed by a self-assembled monolayer (SAM) of an aromatic thiol, 4-aminothiophenol (4-ATP). This electrode has a limit of detection (LOD) of 0.49 μM and a sensitivity of 0.0076 ± 0.0006 μM/μA in cell culture media, which indicates that it retains its selectivity. The applicability of this NADH sensor was demonstrated for the first time by cell viability monitoring via NADH-sensing in cell culture supernatants.

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“…Therefore, the electrochemical performance of the resulting Bio-Nano-PEDOT-based biosensor was mainly dominated by the net effect of the nano-structure morphology. NADH was chosen as the model to evaluate the biomolecules transducing property at the Nano-PEDOT-COOH interface, since NADH is one of the most important co-enzymes in the human body, and can be potentially coupled to a wide range of NADH-dependent dehydrogenases for applications in biosensing and bioelectrocatalysis (Meng et al 2018). Fig.…”

Section: Effect On the Nanofibrous Structured Pedot On Nadh Sensingmentioning

confidence: 99%

Tunable 3D nanofibrous and bio-functionalised PEDOT network explored as a conducting polymer-based biosensor

Meng

Turner

Mak

2020

Biosensors and Bioelectronics

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Conducting polymers that possess good electrochemical properties, nanostructured morphology and functionality for bioconjugation are essential to realise the concept of all-polymer-based biosensors that do not depend on traditional nanocatalysts such as carbon materials, metal, metal oxides or dyes. In this research, we demonstrated a facile approach for the simultaneous preparation of a bi-functional PEDOT interface with a tunable 3D nanofibrous network and carboxylic acid groups (i.e. Nano-PEDOT-COOH) via controlled co-polymerisation of EDOT and EDOT-COOH monomers, using tetrabutylammonium perchlorate as a soft-template. By tuning the ratio between EDOT and EDOT-COOH monomer, the nanofibrous structure and carboxylic acid functionalisation of Nano-PEDOT-COOH were varied over a fibre diameter range of 15.6 ± 3.7 to 70.0 ± 9.5 nm and a carboxylic acid group density from 0.03 to 0.18 µmol cm -2 . The nanofibres assembled into a three-dimensional network with a high specific surface area, which contributed to low charge transfer resistance and high transduction activity towards the co-enzyme NADH, delivering a wide linear range of 20960 M and a high sensitivity of 0.224 A M -1 cm -2 at the Nano-PEDOT-COOH50% interface. Furthermore, the carboxylic acid groups provide an anchoring site for the stable immobilisation of an NADH-dependent dehydrogenase (i.e. lactate dehydrogenase), via EDC/S-NHS chemistry, for the fabrication of a Bio-Nano-PEDOT-based biosensor for lactate detection which had a response time of less than 10 s over the range of 0.051.8 mM. Our developed bio-Nano-PEDOT interface shows future

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Positively-charged hierarchical PEDOT interface with enhanced electrode kinetics for NADH-based biosensors

Cited by 30 publications

References 60 publications

Large‐Scale Synthesis of Functionalized Nanowires to Construct Nanoelectrodes for Intracellular Sensing

Large‐Scale Synthesis of Functionalized Nanowires to Construct Nanoelectrodes for Intracellular Sensing

Non-Destructive Monitoring via Electrochemical NADH Detection in Murine Cells

Tunable 3D nanofibrous and bio-functionalised PEDOT network explored as a conducting polymer-based biosensor

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