Positively-charged electrosprayed nanoparticles based on biodegradable polymers containing amphotericin B for the treatment of leishmaniasis

Messeder, Manuela Maria de Sousa; Miranda, Diego Oliveira; Souza, Sarah Oliveira Lamas de; Dorneles, Matheus Figueiredo; Giunchetti, Rodolfo Cordeiro; Oréfice, Rodrigo Lambert

doi:10.1080/00914037.2020.1785457

Cited by 9 publications

(2 citation statements)

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“…The FTIR spectra of AMB, PCL NPs, and PCL-AMB NPs are shown in Figure 3 A. In PCL NPs, two bands occurred at 2945 cm −1 and 2868 cm −1 which were attributed to the symmetrical and asymmetric axial deformations of the CH 2 group, respectively [ 32 ]. The most prominent peak observed at 1722 cm −1 can be credited to the axial deformation signal of the carbonyl (C=O) present in the ester group.…”

Section: Resultsmentioning

confidence: 99%

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Lipase-Responsive Amphotericin B Loaded PCL Nanoparticles for Antifungal Therapies

et al. 2022

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Amphotericin B is an antifungal drug used for the treatment of invasive fungal infections. However, its clinical use is limited due to its serious side effects, such as renal and cardiovascular toxicity. Furthermore, amphotericin B is administered in high doses due to its poor water solubility. Hence, it is necessary to develop an on-demand release strategy for the delivery of amphotericin B to reduce cytotoxicity. The present report describes a novel encapsulation of amphotericin B into lipase-sensitive polycaprolactone to form a nanocomposite. Nanocomposites were produced by the oil-in-water method and their physicochemical properties such as size, hydrodynamic diameter, drug loading, and zeta potential were determined. The in vitro release of amphotericin B was characterized in the presence and absence of lipase. The antifungal activity of the nanocomposites was verified against lipase-secreting Candida albicans, and cytotoxicity was tested against primary human dermal fibroblasts. In the absence of lipase, the release of amphotericin B from the nanocomposites was minimal. However, in the presence of lipase, an enzyme that is abundant at infection sites, a fungicidal concentration of amphotericin B was released from the nanocomposites. The antifungal activity of the nanocomposites showed an enhanced effect against the lipase-secreting fungus, Candida albicans, in comparison to the free drug at the same concentration. Furthermore, nanoencapsulation significantly reduced amphotericin B-related cytotoxicity compared to the free drug. The synthesized nanocomposites can serve as a potent carrier for the responsive delivery of amphotericin B in antifungal applications.

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Section: Resultsmentioning

confidence: 99%

“…The most prominent peak observed at 1722 cm −1 can be credited to the axial deformation signal of the carbonyl (C=O) present in the ester group. The C–O–C asymmetric stretch occurs at 1238 cm −1 while the C–O–C symmetric stretch occurs at 1165 cm -1 , both indicative of PCL [ 32 , 33 ].…”

Section: Resultsmentioning

confidence: 99%