Positive selection of a T-cell subpopulation in the thymus in which it develops.

Iwabuchi, Kazuya; Negishi, Izumi; Arase, Hisashi; Iwabuchi, Chikako; Ogasawara, Kunio; Good, Robert A.; Onoé, Kazunori

doi:10.1073/pnas.86.13.5089

Cited by 15 publications

(7 citation statements)

References 30 publications

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“…[HE staining (A, B, G, and H) ϭ ϫ40; immunohistochemical staining (C, D, E, and F) ϭ ϫ400. ] of the thymus (15,16,(25)(26)(27)(28)(29), although it has been shown that macrophages and dendritic cells derived from BM precursors were partially involved in functional maturation of thymocytes (30). The present findings demonstrated once again that the developmental arrest of NK1.1 ϩ TCR-␣͞␤ ϩ thymocytes in aly͞aly mouse is associated with structural abnormalities of the thymus that are peculiar to the aly͞aly mouse.…”

Section: Nk11supporting

confidence: 53%

Generation of NK1.1⁺T cell antigen receptor α/β⁺thymocytes associated with intact thymic structure

Nakagawa

Iwabuchi

Ogasawara

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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following correction should be noted. On page 2475, the second line of the left column that reads ". . . were reconstituted with aly͞aly BM cells after lethal irradiation" should read ". . . were reconstituted with aly͞ϩ BM cells after lethal irradiation."Immunology. In the article "Modifying the sequence of an immunoglobulin V-gene alters the resulting pattern of hypermutation," by Beatriz Goyenechea and César Milstein, which appeared in number 24, November 26, 1996, of Proc. Natl. Acad. Sci. USA (93, 13979-13984), the following revision should be noted: The formula at the bottom of the first column of page 13980 was misprinted. The correct formula is:FIG. 6. Three-dimensional structure of rQR1 in complex with FAD and NADP ϩ (12). The C-terminal 43 amino acids (232-274) are shown in red (Left), and have been truncated at the arrow (Right). Different colors of the backbone indicate the two different subunits. It can be seen that the truncated portion of QR2 is critical for binding of the pyrophosphate moiety of the NAD(P)H cofactor. Note that the contact regions between the enzyme and FAD are presumably preserved in QR2 (Right), consistent with the tight binding of FAD. The structural comparison between rQR1 and hQR2 is appropriate since the homology between rQR1 and hQR1 is very high (85% identity). CorrectionsProc. Natl. Acad. Sci. USA 94 (1997) Contributed by Robert A. Good, January 3, 1997 ABSTRACTThe development of T cells within the thymus is largely dependent on intact cortical and medullary epithelial cells. However, it has been reported that positive selection of natural killer antigen 1.1 ؉ (NK1.1 ؉ ) T cell antigen receptor (TCR)-␣͞␤ ؉ thymocytes recently identified among CD4 ؉ 8

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Section: Nk11supporting

confidence: 53%

Generation of NK1.1⁺T cell antigen receptor α/β⁺thymocytes associated with intact thymic structure

Nakagawa

Iwabuchi

Ogasawara

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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“…We found that the proportion of Vg6+ thymocytes in CD4-CD8+ SP population of [SWR+BlO.A(4R)] thymus was markedly greater than that of [SWR + BlO] thymus (Negishi et al unpublished observation). Furthermore, we and others have shown that MHC products expressed on epithelial cells in the cortex but not those on medullary M 0 andor dendritic cells are crucial to positive selection in the thymus [25,[38][39][40].Thus,Vg6+ thymocytes derived from SWR mice might have been expanded in the presence of H-2k products expressed on thymic epithelial cells of AKR recipients. This point should be pursued in further analyses which are now being undertaken in our laboratories.…”

Section: Discussionmentioning

confidence: 81%

Cell components required for deletion of an autoreactive T cell repertoire

et al. 1990

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T cells become tolerant to self antigens during their development in the thymus. Clonal deletion of thymocytes bearing T cell receptor (TcR) which recognize self antigens is a major mechanism for generating tolerance. In the present study we have used allogeneic bone marrow (BM) chimeras, prepared with various combinations of mouse strains and focusing especially on expressions of I-E molecules and Mls-1a antigens on the cell surface, to investigate both immunohistochemically and by flow cytometry the cell components that contribute to the clonal deletion of T cells positive for V beta 6 TcR. The V beta 6 TcR expression is strongly associated with T cell recognition of both I-E and Mls-1a antigens. We found that I-E+ cells derived from donor BM (and thus not of recipient lineage) represented a primary requirement for deletion of Mls-1a-reactive thymocytes which bear V beta 6 TcR. Immunohistochemical analysis revealed that the donor-derived I-E+ cells were distributed mainly to the thymic medulla and that the V beta 6+ cells were eliminated from the thymic medulla between 2 and 3 weeks following BM transplantation. In contrast, Mls-1a+ cells of either donor or recipient origin might be responsible for the deletion, even though cortical epithelial cells appeared not to express Mls-1a antigens.

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“…Thymic LDAC as well as thymocytes of the BM chimeras were fully reconstituted by cells derived from donor BM as described previously (22,24,28,42,57). In general, macrophage functions (phagocytosis, esterase activity, expression of Fc or C3 receptors, and granuloma formation) in peripheral tissues are intact in BM chimeras (25,26,57).…”

Section: Discussionmentioning

confidence: 99%

“…This depression was attributed to the thymic environment of the recipient, C3H, mice. T cells differentiate in thymus to immunocompetent matu- chimeras may serve as a useful experimental milieu for further analyses of the thymic stromal functions (4, 5, 13,24,33,37,46,51,52).…”

mentioning

confidence: 99%

Comparative Analyses of Thymocyte and Thymic Low‐Density Adherent Cell Functions

Wambua

Iwabuchi

et al. 1994

Microbiology and Immunology

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Thymocytes which have developed in the C3H thymus showed depressed proliferative responses to stimulation with anti‐CD3 antibody as compared with those which have developed in the thymus of other strains of mice (i.e. AKR). The present study was conducted to analyze immunological functions of the thymic stromal cell population (low‐density adherent cells, LDAC) in the C3H mice using allogeneic bone marrow (BM) chimeras established by BM transplantation in the reciprocal combination of AKR and C3H mice as donor or recipient. The thymic LDAC from C3H mice or the [AKR(donor)→C3H(recipient)] chimeras contained a high proportion of Mac‐1+ cells as compared to AKR mice or the [C3H→AKR] chimeras. The proportion of Mac‐1+ cells paralleled the IL‐1‐ and PGE2‐secreting ability of the LDAC cultured either in the presence or absence of LPS and also paralleled the antigen‐presenting cell functions of the LDAC. Furthermore, after anti‐CD3 stimulation the PGE2 inhibited more profoundly proliferative responses of [AKR→C3H] or normal C3H thymocytes than those of the [C3H→AKR] chimera or normal AKR thymocytes. A PGE2 inhibitor, indomethacin, reversed the depressed responses of the thymocytes which had developed in the C3H thymus. These findings suggest that the lower responsiveness of thymocytes from [AKR→C3H] chimeras to anti‐CD3 stimulation may be attributable to large amounts of PGE2 secreted by LDAC and/or to increased sensitivity of thymocytes themselves to PGE2.

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Positive selection of a T-cell subpopulation in the thymus in which it develops.

Abstract: In SWR mice the expression with highdensity Vp17a (high

Cited by 15 publications

References 30 publications

Generation of NK1.1⁺T cell antigen receptor α/β⁺thymocytes associated with intact thymic structure

Generation of NK1.1⁺T cell antigen receptor α/β⁺thymocytes associated with intact thymic structure

Cell components required for deletion of an autoreactive T cell repertoire

Comparative Analyses of Thymocyte and Thymic Low‐Density Adherent Cell Functions

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Positive selection of a T-cell subpopulation in the thymus in which it develops.

Abstract: In SWR mice the expression with highdensity Vp17a (high

Cited by 15 publications

References 30 publications

Generation of NK1.1+T cell antigen receptor α/β+thymocytes associated with intact thymic structure

Generation of NK1.1+T cell antigen receptor α/β+thymocytes associated with intact thymic structure

Cell components required for deletion of an autoreactive T cell repertoire

Comparative Analyses of Thymocyte and Thymic Low‐Density Adherent Cell Functions

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Generation of NK1.1⁺T cell antigen receptor α/β⁺thymocytes associated with intact thymic structure

Generation of NK1.1⁺T cell antigen receptor α/β⁺thymocytes associated with intact thymic structure