Positive Regulation of Adenylyl Cyclase Activity by a GαiHomolog inNeurospora crassa

Ivey, F. Douglas; Yang, Qi; Borkovich, Katherine A.

doi:10.1006/fgbi.1998.1101

Cited by 68 publications

(100 citation statements)

References 85 publications

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“…Carotenoid accumulation has been shown to be negatively correlated with cAMP levels (138). In accord with these findings, cAMP levels in the ⌬gna-1 mutants are lower than those in the wild type, and levels of cAMP in the gna-1 R178C and gna-1 Q204L mutant strains are higher (120,316). In vitro experiments have demonstrated that GNA1 positively regulates adenylyl cyclase activity, possibly via direct interactions with adenylyl cyclase (120).…”

Section: Camp Signaling Pathwaymentioning

confidence: 68%

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Signal Transduction Cascades Regulating Fungal Development and Virulence

Lengeler

Davidson

D’Souza

et al. 2000

Microbiol Mol Biol Rev

831

774

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SUMMARY Cellular differentiation, mating, and filamentous growth are regulated in many fungi by environmental and nutritional signals. For example, in response to nitrogen limitation, diploid cells of the yeast Saccharomyces cerevisiae undergo a dimorphic transition to filamentous growth referred to as pseudohyphal differentiation. Yeast filamentous growth is regulated, in part, by two conserved signal transduction cascades: a mitogen-activated protein kinase cascade and a G-protein regulated cyclic AMP signaling pathway. Related signaling cascades play an analogous role in regulating mating and virulence in the plant fungal pathogen Ustilago maydis and the human fungal pathogens Cryptococcus neoformans and Candida albicans. We review here studies on the signaling cascades that regulate development of these and other fungi. This analysis illustrates both how the model yeast S. cerevisiae can serve as a paradigm for signaling in other organisms and also how studies in other fungi provide insights into conserved signaling pathways that operate in many divergent organisms.

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Section: Camp Signaling Pathwaymentioning

confidence: 68%

“…Disruption of the gna-1 gene causes pleiotropic defects in asexual and sexual development (119,120,316). During vegetative growth, ⌬gna-1 strains exhibit slower apical extension of hyphae and slower growth, and hyperbranching of the mycelium occurs on solid minimal medium (119).…”

Section: Camp Signaling Pathwaymentioning

confidence: 99%

Signal Transduction Cascades Regulating Fungal Development and Virulence

Lengeler

Davidson

D’Souza

et al. 2000

Microbiol Mol Biol Rev

831

774

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“…While the Dgsa2Dgsa3 mutant is fully fertile concerning fruiting body development, the triple mutant is almost sterile showing a drastically reduced number of perithecia, thus confirming a functional interaction of the Ga-subunit GSA1 and the adenylyl cyclase SAC1 in fruiting body development. Interestingly, a direct regulation of adenylyl cyclase activity through the corresponding Ga-subunit GNA-1 from N. crassa has already been suggested (Ivey et al 1999;Kays and Borkovich 2004). The involvement of both parallel pathways in fruiting body formation is verified by the sterile phenotype of the Dgsa3Dste12 double mutant, which only develops protoperithecia.…”

Section: Discussionmentioning

confidence: 91%

Three α-Subunits of Heterotrimeric G Proteins and an Adenylyl Cyclase Have Distinct Roles in Fruiting Body Development in the Homothallic Fungus Sordaria macrospora

et al. 2008

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Sordaria macrospora, a self-fertile filamentous ascomycete, carries genes encoding three different a-subunits of heterotrimeric G proteins (gsa, G protein Sordaria alpha subunit). We generated knockout strains for all three gsa genes (Dgsa1, Dgsa2, and Dgsa3) as well as all combinations of double mutants. Phenotypic analysis of single and double mutants showed that the genes for Ga-subunits have distinct roles in the sexual life cycle. While single mutants show some reduction of fertility, double mutants Dgsa1Dgsa2 and Dgsa1Dgsa3 are completely sterile. To test whether the pheromone receptors PRE1 and PRE2 mediate signaling via distinct Ga-subunits, two recently generated Dpre strains were crossed with all Dgsa strains. Analyses of the corresponding double mutants revealed that compared to GSA2, GSA1 is a more predominant regulator of a signal transduction cascade downstream of the pheromone receptors and that GSA3 is involved in another signaling pathway that also contributes to fruiting body development and fertility. We further isolated the gene encoding adenylyl cyclase (AC) (sac1) for construction of a knockout strain. Analyses of the three DgsaDsac1 double mutants and one Dgsa2Dgsa3Dsac1 triple mutant indicate that SAC1 acts downstream of GSA3, parallel to a GSA1-GSA2-mediated signaling pathway. In addition, the function of STE12 and PRO41, two presumptive signaling components, was investigated in diverse double mutants lacking those developmental genes in combination with the gsa genes. This analysis was further completed by expression studies of the ste12 and pro41 transcripts in wild-type and mutant strains. From the sum of all our data, we propose a model for how different Ga-subunits interact with pheromone receptors, adenylyl cyclase, and STE12 and thus cooperatively regulate sexual development in S. macrospora.

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“…Furthermore, Neurospora wild-type strains accumulate cAMP in the extracellular medium 49 , although a role in extracellular signalling has not been established. Taken together, these data suggest the possibility that cAMP or a related molecule may serve as an extracellular signal in Neurospora.…”

Section: A New Family Of G-protein-coupled Receptorsmentioning

confidence: 99%

The genome sequence of the filamentous fungus Neurospora crassa

Galagan

Calvo

Borkovich

et al. 2003

Nature

Self Cite

1,498

1,226

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Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. Here, we report a high-quality draft sequence of the N. crassa genome. The approximately 40-megabase genome encodes about 10,000 protein-coding genes-more than twice as many as in the fission yeast Schizosaccharomyces pombe and only about 25% fewer than in the fruitfly Drosophila melanogaster. Analysis of the gene set yields insights into unexpected aspects of Neurospora biology including the identification of genes potentially associated with red light photobiology, genes implicated in secondary metabolism, and important differences in Ca(2+) signalling as compared with plants and animals. Neurospora possesses the widest array of genome defence mechanisms known for any eukaryotic organism, including a process unique to fungi called repeat-induced point mutation (RIP). Genome analysis suggests that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes

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Positive Regulation of Adenylyl Cyclase Activity by a GαiHomolog inNeurospora crassa

Cited by 68 publications

References 85 publications

Signal Transduction Cascades Regulating Fungal Development and Virulence

Signal Transduction Cascades Regulating Fungal Development and Virulence

Three α-Subunits of Heterotrimeric G Proteins and an Adenylyl Cyclase Have Distinct Roles in Fruiting Body Development in the Homothallic Fungus Sordaria macrospora

The genome sequence of the filamentous fungus Neurospora crassa

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