Positive feedback regulation of a Lycium chinense-derived VDE gene by drought-induced endogenous ABA, and over-expression of this VDE gene improve drought-induced photo-damage in Arabidopsis

Guan, Chunfeng; Ji, Jing; Zhang, Xuqiang; Li, Xiaozhou; Chen, Jin; Guan, Wenzhu; Wang, Gang

doi:10.1016/j.jplph.2014.06.022

Cited by 30 publications

(13 citation statements)

References 52 publications

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“…A feed‐forward regulation by Asc on the VTC2 transcript would be rather unusual, because metabolic pathways are most often negatively feedback‐regulated by their end‐products (reviewed by Farré et al ., ). However, some plant hormones, such as abscisic acid can show positive feedback regulation by activating some of its biosynthetic genes (Xiong & Zhu, ; Yang & Tan, ; Guan et al ., ). We, therefore, hypothesize that a feed‐forward loop in Asc biosynthesis enhances stress adaptation responses in Chlamydomonas .…”

Section: Discussionmentioning

confidence: 97%

Regulation of ascorbate biosynthesis in green algae has evolved to enable rapid stress‐induced response via the VTC2 gene encoding GDP‐l‐galactose phosphorylase

et al. 2017

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Ascorbate (vitamin C) plays essential roles in stress resistance, development, signaling, hormone biosynthesis and regulation of gene expression; however, little is known about its biosynthesis in algae. In order to provide experimental proof for the operation of the Smirnoff-Wheeler pathway described for higher plants and to gain more information on the regulation of ascorbate biosynthesis in Chlamydomonas reinhardtii, we targeted the VTC2 gene encoding GDP-l-galactose phosphorylase using artificial microRNAs. Ascorbate concentrations in VTC2 amiRNA lines were reduced to 10% showing that GDP-l-galactose phosphorylase plays a pivotal role in ascorbate biosynthesis. The VTC2 amiRNA lines also grow more slowly, have lower chlorophyll content, and are more susceptible to stress than the control strains. We also demonstrate that: expression of the VTC2 gene is rapidly induced by H O and O resulting in a manifold increase in ascorbate content; in contrast to plants, there is no circadian regulation of ascorbate biosynthesis; photosynthesis is not required per se for ascorbate biosynthesis; and Chlamydomonas VTC2 lacks negative feedback regulation by ascorbate in the physiological concentration range. Our work demonstrates that ascorbate biosynthesis is also highly regulated in Chlamydomonas albeit via mechanisms distinct from those previously described in land plants.

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Section: Discussionmentioning

confidence: 97%

Regulation of ascorbate biosynthesis in green algae has evolved to enable rapid stress‐induced response via the VTC2 gene encoding GDP‐l‐galactose phosphorylase

et al. 2017

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“…CAMTA1 could rapidly change broad spectrum of responsive genes of membrane integrity and photosynthetic machinery for challenging drought stress in Arabidopsis 32 . The over expression of gene LcVDE in Arabidopsis can also increase tolerance to drought stress through increasing the maximum quantum yield of primary photochemistry of PSII 33 . Recently, a bHLH transcription factor PebHLH35 from Populus euphratica has been confirmed to confer drought tolerance through regulating stomatal development and photosynthesis in Arabidopsis 34 .…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis highlights the crucial roles of photosynthetic system in drought stress adaptation in upland rice

Zhang

Xiao

2016

Sci Rep

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Drought stress is one of the major adverse environmental factors reducing plant growth. With the aim to elucidate the underlying molecular basis of rice response to drought stress, comparative transcriptome analysis was conducted between drought susceptible rice cultivar Zhenshan97 and tolerant cultivar IRAT109 at the seedling stage. 436 genes showed differential expression and mainly enriched in the Gene Ontology (GO) terms of stress defence. A large number of variations exist between these two genotypes including 2564 high-quality insertion and deletions (INDELs) and 70,264 single nucleotide polymorphism (SNPs). 1041 orthologous gene pairs show the ratio of nonsynonymous nucleotide substitution rate to synonymous nucleotide substitutions rate (Ka/Ks) larger than 1.5, indicating the rapid adaptation to different environments during domestication. GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of positive selection genes suggested that photosynthesis represents the most significant category. The collocation of positively selected genes with the QTLs of photosynthesis and the different photosynthesis performance of these two cultivars further illuminate the crucial function of photosynthesis in rice adaptation to drought stress. Our results also provide fruitful functional markers and candidate genes for future genetic research and improvement of drought tolerance in rice.

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“…This was in agreement with a previous study that demonstrated the role of ABA in protecting the xanthophyll cycle pool and the photosynthetic apparatus from photo-oxidative stress [ 46 ]. More recently, a novel Lycium chinense -derived violaxanthin de-epoxidase ( LcVDE ) gene was characterized and reported to have a close correlation with drought-induced endogenous ABA accumulation [ 48 ]. The relative expression of LcVDE and the de-epoxidation rate of xanthophyll carotenoids and NPQ were found higher under drought conditions.…”

Section: Psii Damage and Role Of Hormonesmentioning

confidence: 99%

“…The relative expression of LcVDE and the de-epoxidation rate of xanthophyll carotenoids and NPQ were found higher under drought conditions. However, these elevations were reduced by the application of a potent ABA inhibitor [ 48 ]. Moreover, transgenic Arabidopsis lines expressing LcVDE showed improved drought tolerance and a slower decrease in the maximum quantum yield of primary photochemistry of PSII ( Fv/Fm ) compared to control plants [ 48 ].…”

Section: Psii Damage and Role Of Hormonesmentioning

confidence: 99%

“…However, these elevations were reduced by the application of a potent ABA inhibitor [ 48 ]. Moreover, transgenic Arabidopsis lines expressing LcVDE showed improved drought tolerance and a slower decrease in the maximum quantum yield of primary photochemistry of PSII ( Fv/Fm ) compared to control plants [ 48 ]. Because violaxanthin de-epoxidase (VDE) plays an important role in the conversion of violaxanthin to zeaxanthin and violaxanthin serves as a precursor for ABA biosynthesis, it can be speculated that ABA could regulate the expression of photosynthetic genes under drought stress ( Figure 1 ).…”

Section: Psii Damage and Role Of Hormonesmentioning

confidence: 99%

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Current Understanding of the Interplay between Phytohormones and Photosynthesis under Environmental Stress

Gururani

Mohanta

Bae

2015

IJMS

116

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Abiotic stress accounts for huge crop losses every year across the globe. In plants, the photosynthetic machinery gets severely damaged at various levels due to adverse environmental conditions. Moreover, the reactive oxygen species (ROS) generated as a result of stress further promote the photosynthetic damage by inhibiting the repair system of photosystem II. Earlier studies have suggested that phytohormones are not only required for plant growth and development, but they also play a pivotal role in regulating plants’ responses to different abiotic stress conditions. Although, phytohormones have been studied in great detail in the past, their influence on the photosynthetic machinery under abiotic stress has not been studied. One of the major factors that limits researchers fromelucidating the precise roles of phytohormones is the highly complex nature of hormonal crosstalk in plants. Another factor that needs to be elucidated is the method used for assessing photosynthetic damage in plants that are subjected to abiotic stress. Here, we review the current understanding on the role of phytohormones in the photosynthetic machinery under various abiotic stress conditions and discuss the potential areas for further research.

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Positive feedback regulation of a Lycium chinense-derived VDE gene by drought-induced endogenous ABA, and over-expression of this VDE gene improve drought-induced photo-damage in Arabidopsis

Cited by 30 publications

References 52 publications

Regulation of ascorbate biosynthesis in green algae has evolved to enable rapid stress‐induced response via the VTC2 gene encoding GDP‐l‐galactose phosphorylase

Regulation of ascorbate biosynthesis in green algae has evolved to enable rapid stress‐induced response via the VTC2 gene encoding GDP‐l‐galactose phosphorylase

Comparative transcriptome analysis highlights the crucial roles of photosynthetic system in drought stress adaptation in upland rice

Current Understanding of the Interplay between Phytohormones and Photosynthesis under Environmental Stress

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