Positive Effects of Qing’e Pill (青娥丸) on Trabecular Microarchitecture and its Mechanical Properties in Osteopenic Ovariectomised Mice

Shuai, Bo; Zhu, Rui; Yang, Yanping; Shen, Lin; Xu, Xizhen; Ma, Chen; Lü, Lin

doi:10.1007/s11655-016-2604-0

Cited by 8 publications

(8 citation statements)

References 33 publications

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“…Icariin, the active ingredient of Epimedium , increased osteoblastic differentiation by activating the cAMP/PKA/CREB pathway ( Chen et al, 2019 ). According to the previous study, QEP has the effect of increasing β-catenin and BMP2 expression to improve bone health ( Li et al, 2017 ; Shuai et al, 2019 ). In the present study, we found QEP was shown to prevent ferroptosis of osteoblasts and increase osteogenesis in vivo and in vitro .…”

Section: Discussionmentioning

confidence: 99%

Qing`e Pill Inhibits Osteoblast Ferroptosis via ATM Serine/Threonine Kinase (ATM) and the PI3K/AKT Pathway in Primary Osteoporosis

Hao

Bei

et al. 2022

Front. Pharmacol.

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Osteoporosis (OP) is an aging-related disease that is the main etiology of fragility fracture. Qing’e Pill (QEP) is a mixture of traditional Chinese medicine (TCM) consisting of Eucommia ulmoides Oliv., Psoralea corylifolia L., Juglans regia L., and Allium sativum L. QEP has an anti-osteoporosis function, but the underlying mechanism remains unclear. In this study, online databases were employed to determine the chemical compounds of QEP and potential target genes in osteoporosis. Potential pathways associated with genes were defined by Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) databases. A compound–target–disease network was constructed. Hub genes screened through Cytoscape were intersected with the FerrDB database. The potential key genes were validated in HFOB 1.19 cells, and rat models were ovariectomized through Western blot, RT-qPCR, ELISA, HE staining, immunohistochemistry, and immunofluorescence analyses. The intersection targets of QEP and osteoporosis contained 121 proteins, whereas the target–pathway network included 156 pathways. We filtered five genes that stood out in the network analysis for experimental verification. The experiments validated that QEP exerted therapeutic effects on osteoporosis by inhibiting ferroptosis and promoting cell survival via the PI3K/AKT pathway and ATM. In conclusion, combining the application of network analysis and experimental verification may provide an efficient method to validate the molecular mechanism of QEP on osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Qing`e Pill Inhibits Osteoblast Ferroptosis via ATM Serine/Threonine Kinase (ATM) and the PI3K/AKT Pathway in Primary Osteoporosis

Hao

Bei

et al. 2022

Front. Pharmacol.

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“…QEP also affects the mRNA expression of vitamin D receptors in OVX rats, thereby effectively controlling osteoporosis (Shuai et al. 2014 ). In this study, OVX rats exhibited remarkable osteoporotic changes in bone microstructure, including decreased BMD, BV/TV, BS/TV and Tb.N, and increased Tb.Sp.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota and metabonomics used to explore the mechanism of Qing’e Pills in alleviating osteoporosis

Xie

Hua

Guo

et al. 2022

Pharmaceutical Biology

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Context The traditional Chinese medicine Qing’e Pills (QEP) has been used to treat postmenopausal osteoporosis (PMO). Objective We evaluated the regulatory effects of QEP on gut microbiota in osteoporosis. Materials and methods Eighteen female SD rats were divided into three groups: sham surgery (SHAM), ovariectomized (OVX) and ovariectomized treated with QEP (OVX + QEP). Six weeks after ovariectomy, QEP was administered to OVX + QEP rats for eight weeks (4.5 g/kg/day, i.g.). After 14 weeks, the bone microstructure was evaluated. Differences in gut microbiota were analysed via 16S rRNA gene sequencing. Changes in endogenous metabolites were studied using UHPLC-Q-TOF/MS technology. GC–MS was used to detect short-chain fatty acids. Furthermore, we measured serum inflammatory factors, such as IL-6, TNF-α and IFN-γ, which may be related to gut microbiota. Results OVX + QEP exhibited increased bone mineral density (0.11 ± 0.03 vs. 0.21 ± 0.02, p < 0.001) compared to that of OVX. QEP altered the composition of gut microbiota. We identified 19 potential biomarkers related to osteoporosis. QEP inhibited the elevation of TNF-α (38.86 ± 3.19 vs. 29.43 ± 3.65, p < 0.05) and IL-6 (83.38 ± 16.92 vs. 45.26 ± 3.94, p < 0.05) levels, while it increased the concentrations of acetic acid (271.95 ± 52.41 vs. 447.73 ± 46.54, p < 0.001), propionic acid (28.96 ± 5.73 vs. 53.41 ± 14.26, p < 0.01) and butyric acid (24.92 ± 18.97 vs. 67.78 ± 35.68, p < 0.05). Conclusions These results indicate that QEP has potential of regulating intestinal flora and improving osteoporosis. The combination of anti-osteoporosis drugs and intestinal flora could become a new treatment for osteoporosis.

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“…36 It improved cancellous bone microstructure in OVX mice partially through the elevation of β-catenin expression. 37 QEF increased estrogen level, reversed uterine atrophy, and increased ER expression. 38 QEF plus Danshen exerted more evident estrogen-like effects, as revealed by the increased uterus wet weight, the thickness of uterine wall, endometrial epithelium and glandular epithelium, improved trabecular bone and total density, and reduced levels of low-density lipoprotein-cholesterol and triglyceride.…”

Section: Qing E Formulamentioning

confidence: 88%

Chinese herbal formulas for postmenopausal osteoporosis: A review of preclinical evidence on animal studies and molecular mechanism

Lin

Tian

et al. 2018

Tradit. Med. Mod. Med.

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Whether Chinese herbal formulas are effective in treatment of postmenopausal osteoporosis remains unclear. The aim of this study is to explore the experimental evidence of both in vitro and in vivo preclinical studies using Chinese herbal formulas in postmenopausal osteoporosis. Searches were applied to various databases with relevant keywords. Original in vivo and in vitro studies using Chinese herbal formulas to treat postmenopausal osteoporosis, and with full text available, were included. Er-Xian Decoction, Bu-Shen-Ning-Xin Decoction, Qing E Formula, Liuwei Dihuang Wan, and Xian-Ling-Gu-Bao Decoction, the most commonly studied formulas, were selected from the pool of Chinese medicine. The preclinical data indicated the potential use of Chinese herbal formulas in postmenopausal osteoporosis. The underlying mechanisms included bone morphogenetic protein (BMP), Wnt/[Formula: see text]-catenin, extracellular-signal-regulated kinase/c-Jun [Formula: see text] terminal kinase/mitogen-activated protein kinase (ERK/JNK/MAPK), estrogen receptor (ER), and osteoprotegerin/receptor activator of nuclear factor [Formula: see text]B ligand (OPG/RANKL) signaling pathways. This study demonstrated the anti-osteoporotic effect of Chinese herbal formulas targeting different pathways in bone metabolism. Further study with adequate sample size and follow-up time, appropriate controls, and optimal blinding is required.

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Positive Effects of Qing’e Pill (青娥丸) on Trabecular Microarchitecture and its Mechanical Properties in Osteopenic Ovariectomised Mice

Abstract: The increased β-catenin expression may be the mechanism underlying QEP's improvement of the cancellous bone microstructure in ovariectomised mice. Our fifindings provide a scientifific rationale for using QEP as a dietary supplement to prevent bone loss in postmenopausal women.

Cited by 8 publications

References 33 publications

Qing`e Pill Inhibits Osteoblast Ferroptosis via ATM Serine/Threonine Kinase (ATM) and the PI3K/AKT Pathway in Primary Osteoporosis

Qing`e Pill Inhibits Osteoblast Ferroptosis via ATM Serine/Threonine Kinase (ATM) and the PI3K/AKT Pathway in Primary Osteoporosis

Gut microbiota and metabonomics used to explore the mechanism of Qing’e Pills in alleviating osteoporosis

Chinese herbal formulas for postmenopausal osteoporosis: A review of preclinical evidence on animal studies and molecular mechanism

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