Positive effects of proline addition on the central metabolism of wild-type and lactic acid-producing Saccharomyces cerevisiae strains

Nugroho, Riyanto Heru; Yoshikawa, Katsunori; Mizutani, Fumio; Shimizu, Hiroshi

doi:10.1007/s00449-016-1646-1

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…In S. cerevisiae , proline degradation has been reported to be closely associated with riboflavin transport [ 43 , 44 ], while only glycyl-L-proline was downregulated in the metabolome of yellow mushrooms, with a log 2 fold change value reaching −1.32. Furthermore, in S. cerevisiae , proline degradation can activate the PUT3 transcription factor [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Chemical Constituents and Molecular Mechanism of the Yellow Phenotype of Yellow Mushroom (Floccularia luteovirens)

Gan

Bao

Liu

et al. 2022

JoF

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(1) Background: Yellow mushroom (Floccularia luteovirens) is a natural resource that is highly nutritional, has a high economic value, and is found in Northwest China. Despite its value, the chemical and molecular mechanisms of yellow phenotype formation are still unclear. (2) Methods: This study uses the combined analysis of transcriptome and metabolome to explain the molecular mechanism of the formation of yellow mushroom. Subcellular localization and transgene overexpression techniques were used to verify the function of the candidate gene. (3) Results: 112 compounds had a higher expression in yellow mushroom; riboflavin was the ninth most-expressed compound. HPLC showed that a key target peak at 23.128 min under visible light at 444 nm was Vb2. All proteins exhibited the closest relationship with Agaricus bisporus var. bisporus H97. One riboflavin transporter, CL911.Contig3_All (FlMCH5), was highly expressed in yellow mushrooms with a different value (log2 fold change) of −12.98, whereas it was not detected in white mushrooms. FlMCH5 was homologous to the riboflavin transporter MCH5 or MFS transporter in other strains, and the FlMCH5-GFP fusion protein was mainly located in the cell membrane. Overexpression of FlMCH5 in tobacco increased the content of riboflavin in three transgenic plants to 26 μg/g, 26.52 μg/g, and 36.94 μg/g, respectively. (4) Conclusions: In this study, it is clear that riboflavin is the main coloring compound of yellow mushrooms, and FlMCH5 is the key transport regulatory gene that produces the yellow phenotype.

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Section: Discussionmentioning

confidence: 99%

Chemical Constituents and Molecular Mechanism of the Yellow Phenotype of Yellow Mushroom (Floccularia luteovirens)

Gan

Bao

Liu

et al. 2022

JoF

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“…The only amino acid which level was reduced after MCHM treatment was L-proline. In Saccharomyces cerevisiae, L-proline is a stress protectant, effective against oxidative stress under low pH conditions (Nugroho et al, 2016) , protecting against representative inhibitors like furfural, acetic acid and phenol (Wang et al, 2015) and contributing to ethanol tolerance (Ohta et al, 2016) . It is of note that the other protective metabolite against representative inhibitors found by Wang et al (Wang et al, 2015) , myo-inositol, also has a lower level due to MCHM.…”

Section: Discussionmentioning

confidence: 99%

Effects of MCHM on yeast metabolism

Pupo

Gallagher

2019

Preprint

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On January 2014 approximately 10,000 gallons of crude 4-Methylcyclohexanemethanol (MCHM) and propylene glycol phenol ether (PPH) were accidentally released into the Elk River, West Virginia, contaminating the tap water of around 300,000 residents. Crude MCHM is an industrial chemical used as flotation reagent to clean coal. At the time of the spill, MCHM's toxicological data were limited, an issue that have been addressed by different studies focused on understanding the immediate and longterm effects of MCHM on human health and the environment. Using S. cerevisiae as a model organism we study the effect of acute exposition to crude MCHM on metabolism. Yeasts were treated with MCHM 3.9 mM in YPD for 30 minutes. Polar and lipid metabolites were extracted from cells by a chloroform-methanol-water mixture. The extracts were then analyzed by direct injection ESI-MS and by GC-MS. The metabolomics analysis was complemented with flux balance analysis simulations done with genome-scale metabolic network models (GSMNM) of MCHM treated vs non-treated control. We integrated the effect of MCHM on yeast gene expression from RNA-Seq data within these GSMNM. 181 and 66 metabolites were identified by the ESI-MS and GC-MS procedures, respectively. From these 38 and 34 relevant metabolites were selected from ESI-MS and GC-MS respectively, for 72 unique compounds. MCHM induced amino acid accumulation, via its effects on amino acid metabolism, as well as a potential impairment of ribosome biogenesis. MCHM affects phospholipid biosynthesis and decrease the levels of ergosterol, with a potential impact in the biophysical properties of yeast cellular membranes. The FBA simulations were able to reproduce the deleterious effect of MCHM on cell's growth and suggest that the effect of MCHM on ubiquinol:ferricytochrome c reductase reaction, caused by the under-expression of CYT1 gene, could be the driven force behind the observed effect on yeast metabolism and growth.

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Recombinant xylose-fermenting yeast construction for the co-production of ethanol and cis,cis-muconic acid from lignocellulosic biomass

Liu

Peng

Huang

et al. 2020

Bioresource Technology Reports

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Positive effects of proline addition on the central metabolism of wild-type and lactic acid-producing Saccharomyces cerevisiae strains

Cited by 4 publications

References 29 publications

Chemical Constituents and Molecular Mechanism of the Yellow Phenotype of Yellow Mushroom (Floccularia luteovirens)

Chemical Constituents and Molecular Mechanism of the Yellow Phenotype of Yellow Mushroom (Floccularia luteovirens)

Effects of MCHM on yeast metabolism

Recombinant xylose-fermenting yeast construction for the co-production of ethanol and cis,cis-muconic acid from lignocellulosic biomass

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