Positive effects of platelet-rich plasma (PRP) and a Sanguisorba officinalis polysaccharide on the proliferation and differentiation of anterior cruciate ligament (ACL) fibroblasts in vitro

Abstract

Context: Sanguisorba officinalis L. (Rosaceae), a famous traditional Chinese medicine. It was recently reported that its polysaccharide could facilitate collagen production. Objectives: We investigated the mechanism by which S. officinalis polysaccharide (SOWPa) and/or platelet-rich plasma (PRP) promote regenerative potential of anterior cruciate ligament (ACL) in vitro. Materials and methods: ACL fibroblasts were treated with SOWPa (25 and 100 mg/kg), PRP, PRP þ SOWPa (25 and 100 mg/kg) or vehicle alone for 24, 48, or 72 h. Cell viability, migration ability and apoptosis were evaluated by MTT, transwell and flow cytometry, respectively. Western blot analysis was performed to assess associated protein expression. Results: PRP, SOWPa (100 mg/kg) or PRP þ SOWPa (100 mg/kg) treatment for 72 h significantly improved the cell viability of ACL fibroblasts from 100 ± 7.5% (control) to 156.85 ± 12.82%, 188.08 ± 15.92%, and 223.67 ± 18.82%, respectively, which was evidenced by individual decreased apoptosis rate from 31.26 ± 2.35% (control) to 20.80 ± 1.89%, 18.01 ± 1.55% and 9.33 ± 0.78%. Furthermore, the motility of ACL fibroblasts was significantly improved with increased migrated cell number per field from 5 for control to 26 for PRP, 36 for SOWPa and 44 for PRP þ SOWPa, respectively. Moreover, the protein expression of differentiation markers (RUNX2, ALP, BMP2 and Col I) and TLR-4 and phosphorylated p65 (p-p65) was inhibited by the above treatment. Discussion and conclusions: Data suggested that the addition of SOWPa to PRP increased the regenerative ability of ACL fibroblasts by blocking the TLR-4/NF-jB pathway.

tRNA-derived fragment TRF365 regulates the metabolism of anterior cruciate ligament cells by targeting IKBKB

“…A recent study has shown that miRNA-214-3p regulates the NF-κB signalling pathway by targeting IKBKB , and a decrease in miRNA-214-3p expression leads to an increase in IKBKB expression and activation of the NF-κB signalling pathway [ 28 ]. Another study has shown that platelet-rich plasma and Sanguisorba officinalis polysaccharide can increase the regenerative ability of ACL fibroblasts by blocking the TLR4/NF-κB pathway [ 35 ]. To clarify the expression pattern of IKBKB in the ACL, we compared IKBKB expression in normal and OA ACL cells and found that IKBKB was highly expressed in OA ACL cells but not in normal ACL cells.…”

Platelet-rich plasma pretreatment protects anterior cruciate ligament fibroblasts correlated with PI3K-Akt-mTOR pathway under hypoxia condition

“…Zheng et al. [ 31 ] found that PRP could improve the migration abilities of ACL fibroblasts significantly, especially at 72 ​h after cell seeded. The growth factors like TGF-β and FGF, as the component of PRP, have been proved to stimulate cell growth and migration [ 32 , 33 ].…”

Fibrin‐based biomaterial systems to enhance anterior cruciate ligament healing

“…PRP has also been used as a vessel for therapeutic delivery of bioactive agents or cells. For example, Zheng et al (2020) examined the effects of PRP and a Sanguisorba officinalis polysaccharide (SOWPa), a Chinese herb known to enhance collagen deposition, on ACL fibroblasts. Another common PRP administration approach includes the addition of adipose‐derived progenitor cells (ADPCs) or mesenchymal stem cells (MSCs) (often in the form of bone marrow aspirate concentrate [BMAC]).…”