Positive effects of Forskolin (stimulator of lipolysis) treatment on cryosurvival of in vitro matured porcine oocytes

Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

“…Therefore, the oocyte fat indexes were similar to control. The mechanism by which forskolin interfered with LD organization is not well understood but its lypolitic activity in porcine oocytes as well as in embryos has already been identified (Men et al, 2006;Fu et al, 2011). Herein, even when forskolin exposure was only at the initial 2 h of culture, these oocytes at 22 to 24 h had smaller LD and fat areas compared with the control group, although at 44 to 48 h of meiosis these measurements were similar to those of control.…”

“…On the contrary, Fu et al (2011) showed that 10 mM forskolin effectively reduced the fluorescence intensity of porcine oocytes stained with Nile Red as well as the number of LD during IVM. Data presented here demonstrated no differences in LD areas when this lipolytic agent was present during the entire maturation period or till 22 to 24 h, but fat areas were reduced (exp.…”

“…In accordance, since at 44 to 48 h of culture, GV configuration continued to be predominant in forskolintreated oocytes, remaining their areas similar to immature ones, these oocytes were incapable of normal fertilization and cleavage (0%). In contrast, Fu et al (2011) achieved a cleavage rate of 64.5 6 3.6% in parthenogenetically activated porcine oocytes cultured with 10 mM forskolin during the entire IVM period. An increase in cytoplasmic calcium during parthenogenic activation was referred to be sufficient to induce low to moderate extents of oocyte activation events such as cell cycle resumption (Gardner and Evans, 2006).…”

“…Forskolin, an adenylyl cyclase stimulator, at 10 mM dose is an effective lipolytic substance capable of improving oocyte and embryo cryosurvival (Men et al, 2006;Fu et al, 2011). However, despite the contribution of forskolin to lipid reduction, it can simultaneously arrest meiotic resumption during porcine oocytes IVM in a dose-dependent response.…”

Factors Affecting Cryopreservation of Porcine Oocytes

“…A non-invasive approach to reduce intracellular lipid content by the use of Forskolin, a lipolysis-inducing reagent has been reported to improve the cryotolerance of porcine blastocysts [74]. Forskolin treatment during IVM has also been found to improve cryotolerance of matured porcine oocytes; however, long term treatment with this reagent seems to exert negative side effects on oocyte competence [75] presumably by raising the levels of cAMP in oocyte cytoplasm to abnormally high levels. Another reagent, L-carnitine can also reduce the amount of intracellular lipid in oocytes during IVM without reducing their developmental competence [76].…”