Positive effect of collagen V and VI on triglyceride accumulation during differentiation in cultures of bovine intramuscular adipocytes

Nakajima, Ikuyo; Muroya, Susumu; Tanabe, Ryo; Chikuni, Koichi

doi:10.1046/j.1432-0436.2002.700203.x

Cited by 59 publications

(56 citation statements)

References 33 publications

(37 reference statements)

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“…39,40 Collagen, in particular type V and VI, which is newly synthesized and organized by adipocytes during differentiation, seems to be necessary for triglyceride accumulation. 41 Selected procollagen genes were highly expressed in adipocytes compared with macrophages also in our study (Figure 4) emphasizing that adipocytes themselves remodel their surrounding matrix under high-fat diet feeding. In addition to matrix component genes, expression of arginase type I that provides proline precursors for collagen production [42][43][44] was strongly upregulated in gonadal adipose tissue of db/db mice fed HF/S compared with those on LF but this upregulation was prevented by n-3 PUFA (data not shown).…”

Section: Discussionsupporting

confidence: 71%

Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids

Huber

Löffler

Bilban³

et al. 2006

Int J Obes

127

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Section: Discussionsupporting

confidence: 71%

Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids

Huber

Löffler

Bilban³

et al. 2006

Int J Obes

127

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“…2A). Intriguingly, type V collagens have been previously shown to induce proliferation and differentiation of preadipocytes in vitro (19,20) and regulate collagen fibrillogenesis to control ECM geometry and tensile strength (16,17), properties that have been implicated in metabolic dysfunction of obese AT (28). Immunofluorescence confirmed an increase in Col5 protein and revealed specific localization to vascular endothelial cells in plxnd1 mutant VAT (Fig.…”

Section: Col5a1 Is Induced By Vascular Endothelial Cells Of Plxnd1 Mumentioning

confidence: 65%

“…In turn, ECM provides a supportive microenvironment for AT growth and function (15). For example, type V collagens regulate collagen fiber assembly, geometry, and strength (16,17), are up-regulated during adipogenesis, and can stimulate adipocyte differentiation in vitro (18)(19)(20). However, the role of type V collagens during in vivo AT growth is unknown.…”

mentioning

confidence: 99%

Plexin D1 determines body fat distribution by regulating the type V collagen microenvironment in visceral adipose tissue

Minchin

Dahlman

Harvey

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Genome-wide association studies have implicated PLEXIN D1 (PLXND1) in body fat distribution and type 2 diabetes. However, a role for PLXND1 in regional adiposity and insulin resistance is unknown. Here we use in vivo imaging and genetic analysis in zebrafish to show that Plxnd1 regulates body fat distribution and insulin sensitivity. Plxnd1 deficiency in zebrafish induced hyperplastic morphology in visceral adipose tissue (VAT) and reduced lipid storage. In contrast, subcutaneous adipose tissue (SAT) growth and morphology were unaffected, resulting in altered body fat distribution and a reduced VAT:SAT ratio in zebrafish. A VAT-specific role for Plxnd1 appeared conserved in humans, as PLXND1 mRNA was positively associated with hypertrophic morphology in VAT, but not SAT. In zebrafish plxnd1 mutants, the effect on VAT morphology and body fat distribution was dependent on induction of the extracellular matrix protein collagen type V alpha 1 (col5a1). Furthermore, after high-fat feeding, zebrafish plxnd1 mutant VAT was resistant to expansion, and excess lipid was disproportionately deposited in SAT, leading to an even greater exacerbation of altered body fat distribution. Plxnd1-deficient zebrafish were protected from high-fat-diet-induced insulin resistance, and human VAT PLXND1 mRNA was positively associated with type 2 diabetes, suggesting a conserved role for PLXND1 in insulin sensitivity. Together, our findings identify Plxnd1 as a novel regulator of VAT growth, body fat distribution, and insulin sensitivity in both zebrafish and humans.zebrafish | body fat distribution | adipose development | insulin resistance | extracellular matrix T he regional distribution and morphology of adipose tissue (AT) are strong predictors of metabolic disease (1-3). Excess lipid deposition in visceral AT (VAT; adipose associated with visceral organs) is associated with increased susceptibility to insulin resistance and type 2 diabetes (4), whereas expansion of subcutaneous AT (SAT; adipose between muscle and skin) is associated with reduced risk for metabolic disease and is even protective against hyperglycemia and dyslipidemia (4-7). In turn, hypertrophic AT morphology (few large adipocytes) is associated with insulin resistance and AT dysfunction, whereas hyperplastic AT morphology (many small adipocytes) is associated with improved metabolic parameters (4, 7-9). Therefore, the identification of factors that regulate regional distribution and AT morphology could lead to new therapies to treat metabolic disease.Genome-wide association studies have implicated the PLEXIN D1 (PLXND1) locus in waist:hip ratio (a measurement of regional AT distribution) and type 2 diabetes (10). However, a role for Plxnd1 in AT morphology, distribution, and metabolism is unknown. Plxnd1 is a transmembrane receptor that controls the migration, proliferation, and survival of diverse cell types (11).Mutation of Plxnd1 in mouse and zebrafish leads to hypervascularization in many tissues (12, 13), and vascular endothelial cell Plxnd1 modulates extracellu...

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“…Indeed, adipogenesis is characterized by the conversion of a fibronectin-rich matrix to a basement membrane (Gregoire, 2001). During adipocyte differentiation, there is an increase in the secretion of basement membrane components such as type V and VI collagens (Nakajima et al, 2002) (Iyengar et al, 2005). Thus, in a general fashion, normal mature adipocytes are separated from epithelial cells by basement membranes, limiting therefore the possibilities of heterotypic cross-talk between the 2 cell types.…”

Section: The Role Of Adipocytes In the Normal Breastmentioning

confidence: 99%

Adipocyte is a non-trivial, dynamic partner of breast cancer cells

Tan

Buache²,

Chenard³

et al. 2011

Int. J. Dev. Biol.

147

142

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While the participation of adipocytes is well known in tissue architecture, energy supply and endocrine processes, their implication during natural cancer history is just beginning to unfold. An extensive review of the literature concerning the impact of resident adipocytes on breast cancer development/progression was performed. This review provides in vitro and in vivo evidence that adipocytes located close to invasive cancer cells, referred to as cancer-associated adipocytes (CAAs), are essential for breast tumor development/progression. Their deleterious function is dependent, at least partly, on their crosstalk with invasive cancer cells. Indeed, this event leads to dramatic phenotypic and/or functional modifications of both cell types. Adipocytes exhibit delipidation and acquire a fibroblast-like shape. In parallel, cancer cell aggressiveness is exacerbated through increased migratory and invasive properties. Moreover, obesity is currently a sign of poor prognosis in human carcinomas. In this context, a high number of "obese" resident adipocytes might be predicted to be detrimental. Accordingly, there are some similarities between the molecular alterations observed in hypertrophied adipocytes and in CAAs. How adipocytes function to favor tumorigenesis at the molecular level remains largely unknown. Nevertheless, progress has been made recently and molecular clues are starting to emerge. Deciphering the cellular and molecular mechanisms behind the adipocyte-cancer cell heterotypic crosstalk is of great interest since it might provide new targets for improving diagnosis/prognosis and for the design of innovative therapeutic strategies. They might also improve our understanding of the relationship between obesity/metabolic disorders and cancer risk and/or poor patient outcome.

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Positive effect of collagen V and VI on triglyceride accumulation during differentiation in cultures of bovine intramuscular adipocytes

Cited by 59 publications

References 33 publications

Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids

Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids

Plexin D1 determines body fat distribution by regulating the type V collagen microenvironment in visceral adipose tissue

Adipocyte is a non-trivial, dynamic partner of breast cancer cells

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