Positive Effect of Cannabis sativa L. Herb Extracts on Skin Cells and Assessment of Cannabinoid-Based Hydrogels Properties

Abstract

The skin is an organ that is constantly exposed to many external factors that can affect its structure and function. Due to the presence of different cannabinoid receptors on many types of skin cells, cannabinoids can interact directly with them. Therefore, as part of this work, the impact of two types of Cannabis sativa L. herb extracts on keratinocytes and fibroblasts was assessed. The content of biologically active compounds such as phenols, flavonoids, chlorophylls and cannabinoids was evaluated. The antioxidant capacity of prepared extracts using the DPPH radical, H2DCFDA probe and measurement of superoxide dismutase activity was also assessed. The cytotoxicity of hemp extracts was determined using the Alamar Blue, Neutral Red and LDH assays. The ability of the extracts to inhibit the activity of matrix metalloproteinases, collagenase and elastase, was assessed. Preparations of model hydrogels were also prepared and their effect on transepidermal water loss and skin hydration was measured. The obtained results indicate that hemp extracts can be a valuable source of biologically active substances that reduce oxidative stress, inhibit skin aging processes and positively affect the viability of skin cells. The analysis also showed that hydrogels based on cannabis extracts have a positive effect on skin hydration.

Epilobium angustifolium L. Extracts as Valuable Ingredients in Cosmetic and Dermatological Products

“…Another essential substance in plant extracts is the assimilation pigments. Chlorophyll may also enhance the antioxidant properties [ 44 ]. In our study, we showed the total polyphenol and flavonoids and the assimilation pigments contents in FEE.…”

“…The hydrogel was prepared according to a modified procedure by Zagórska-Dziok et al [ 44 ]. Sequentially, an aqueous solution of hydroxyethyl cellulose (HEC) was prepared.…”

Cannabinoids and Inflammations of the Gut-Lung-Skin Barrier

“…The simple and effective topical administration of cannabinoids on skin lesions is helpful not only in ensuring substance delivery to the inflammation site but also in observing local adverse effects [154]. The additional anti-aging, anti-oxidative and antitumoral effects provide supplementary benefits in the use of cannabinoids in other diseases that are associated with inflammation, including cancer [155,156]. However, many of the studies are preclinical, and there are very few trials free of bias with a large enough number of participants to be considered high-quality [157].…”

Pickering emulsions stabilized with chitosan/collagen peptides nanoparticles as green topical delivery vehicles for cannabidiol (CBD)

“…Recently, cannabis extracts, and CBD in particular, have also gained tremendous attention in the cosmetic industry sector owing to their moisturizing, anti-aging, anti-inflammatory, antioxidant and cytoprotective properties [5,6]. Moreover, recent studies have shown that cannabis extracts do not lead to cytotoxic effects on keratinocytes and skin fibroblasts, proving the safety and non-irritancy of cannabis active ingredients [7,8]. CBD is a highly lipophilic drug (log P = 6.3) and, therefore, it is frequently incorporated in alcohol-based formulations or oils [9].…”