Positive effect of Astragaloside IV on neurite outgrowth via talin‐dependent integrin signaling and microfilament force

Abstract

Integrin plays a prominent role in neurite outgrowth by transmitting both mechanical and chemical signals. Integrin expression is closely associated with Astragaloside IV (AS-IV), the main component extracted from Astragali radix, which has a positive effect on neural-protection. However, the relationship between AS-IV and neurite outgrowth has not been studied exhaustively to date. The present study investigated the underlying mechanism of AS-IV on neurite outgrowth. Longer neurites have been observed in SH-SY5Y cells or cortical neurons after AS-IV treatment. Furthermore, AS-IV not only increased the expression of integrin β but also activated it. The AS-IV-induced increased integrin activity was attributed to the integrin-activating protein talin. Application of the actin force probe showed that AS-IV led to an increase in intracellular microfilament force during neurite growth. Furthermore, in response to AS-IV, the microfilament force was regulated by talin and integrin activity during neurite growth. These results suggest that AS-IV has the ability to increase intracellular structural force and facilitate neurite elongation by integrin signaling, which highlights its therapeutic potential for neurite outgrowth.

Additive Manufacturing of Astragaloside-Containing Polyurethane Nerve Conduits Influenced Schwann Cell Inflammation and Regeneration

“…Furthermore, cells in the Ast10 group were flatter and had elongated spindles at day 1 as compared to the rest of the groups, thus indicating that the microenvironment was more favorable to RSCs. In addition, the RSCs cultured on the conduits with Ast stimulation showed longer axons and more spindle cells, which proved the positive influence of Ast stimulation on neuronal cell proliferation [31]. These data indicated that the addition of Ast can promote cellular adhesion and proliferation and can be used as a neurogenic factor in future nerve-regeneration-related studies.…”

Astragaloside regulates lncRNA LOC100912373 and the miR‑17‑5p/PDK1 axis to inhibit the proliferation of fibroblast‑like synoviocytes in rats with rheumatoid arthritis

“…The pathogenesis of RA is extremely complex and involves environmental, immune, genetic and other factors ( 35 , 36 ), thus rendering the achievement of RA targeted treatment development a difficult research target. Currently, increasing attention has been paid to the role of natural herbal extracts with a probable therapeutic effect on the treatment of several diseases, including RA ( 37 , 38 ). For example, Astragalus membranaceus , Tripterygium wilfordii and Radix Paeoniae Alba have been proven to have certain therapeutic effects on RA ( 39 - 41 ).…”