Positive correlation between the expression of X‐chromosome RBM genes (RBMX, RBM3, RBM10) and the proapoptotic Bax gene in human breast cancer

Martínez-Arribas, Fernando; Agudo, David; Pollán, Marina; Gómez-Esquer, Francisco; Dı́az-Gil, Gema; Lucas, R. Hernanz de; Schneider, J.

doi:10.1002/jcb.20725

Cited by 71 publications

(72 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…27 RBPs have also been described as a novel family of apoptosis modulators, 1 and a correlation between the X chromosomerelated RBM genes (RBMX, RBM3 and RBM10) and the pro-apoptotic Bax gene has been shown in breast cancer. 5 Our data add to these findings by the observation that increased RBM3 expression was associated with favorable clinicopathological variables and an improved prognosis in two independent breast cancer cohorts.…”

Section: Discussionsupporting

confidence: 67%

“…3 The RBM3 protein binds to both DNA and RNA. 4 Although RBM proteins have been proposed to be a novel family of apoptosis regulators, 1,[5][6][7] the exact function of RBM3 has yet to be fully elucidated. RBM3 transcripts have been found in various human tissues, 3 and in vitro, RBM3 is one of the earliest proteins synthesized in response to mild hypothermia.…”

mentioning

confidence: 99%

“…8 A correlation between the X chromosomerelated RBM genes (RBMX, RBM3 and RBM10) and the proapoptotic Bax gene has been shown in breast cancer. 5 Downregulation of RBM3 is associated with melanoma progression; 9 however, a recent report described RBM3 as a proto-oncogene that protects against mitotic catastrophe in colorectal cancer cell lines. 10 Taken together, the role of RBM3 in the context of tumor initiation and progression in various cancer forms remains unknown and, to date, no studies have addressed the role of tumor-specific RBM3 protein expression in relation to disease outcome.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Nuclear expression of the RNA-binding protein RBM3 is associated with an improved clinical outcome in breast cancer

et al. 2009

View full text Add to dashboard Cite

Single-strand RNA-binding proteins (RBPs) are involved in many aspects of RNA metabolism and in the regulation of gene transcription. The RBP RBM3 was recently suggested to be a proto-oncogene in colorectal cancer; however, such a role has not been corroborated by previous studies in the colon or other tumor types, and the prognostic implications of tumor-specific RBM3 expression remain unclear. Mono-specific antibodies against RBM3 were generated. Antibody specificity was confirmed using siRNA gene silencing, western blotting and immunohistochemistry on a panel of breast cancer cell lines. Using tissue microarrays and IHC, RBM3 protein expression was examined in 48 normal tissues and in 20 common cancers. Additional analysis in two independent breast cancer cohorts (n ¼ 1016) with long-term follow-up was also carried out. RBM3 was upregulated in cancer compared to normal tissues. The nuclear expression of RBM3 in breast cancer was associated with low grade (Po0.001), small tumors (Po0.001), estrogen receptor (ER) positivity (Po0.001) and Ki-67 negativity (Po0.001) in both the breast cancer cohorts. An increased nuclear expression of RBM3 was associated with a prolonged overall and recurrence-free survival. The prognostic value was particularly pronounced in hormone receptor-positive tumors and remained significant in multivariate interaction analysis after controlling for tamoxifen treatment (HR: 0.49, 95% CI: 0.30-0.79, P ¼ 0.004). These data strongly indicate that nuclear RBM3 is an independent favorable prognostic factor in breast cancer, and seems to have a specific role in ER-positive tumors.

show abstract

Section: Discussionsupporting

confidence: 67%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Nuclear expression of the RNA-binding protein RBM3 is associated with an improved clinical outcome in breast cancer

et al. 2009

View full text Add to dashboard Cite

show abstract

“…However, this study has only confirmed the involvement of some genes in cancer; others were left to be further studied. To date, there are a small number of studies reporting RBM10-regulated genes, such as Bax, 12 VEGF, 10 EGFR, 15 FAX, 31 TNF, 22 NUMB, 19 or Fas and Bcl-x genes. 5 Nevertheless, this study does have some limitations.…”

Section: Discussionmentioning

confidence: 99%

“…4 RBM10 can be alternatively spliced to produce RBM10 RNA variant 1 and variant 2, which encode nuclear RNA-binding proteins containing zinc finger motifs, a G-patch, and two RNA Recognition Motif (RRMs) to regulate gene transcription, mRNA alternative splicing, and stabilization of various genes, including apoptosis-related Fas and Bcl-x genes. 5 RBM10 is widely expressed in a variety of cells and tissues, 6,7 and altered RBM10 expression has been reported in TARP syndrome (talipes equinovarus, atrial septal defect, Robin sequence, and persistent left superior vena cava syndrome), X-linked disorder, 8,9 and various human cancers, such as breast cancer, [10][11][12] pancreatic cancer, 13 and metastatic melanoma. 14 In lung cancer, a comprehensive molecular profiling study of lung adenocarcinoma demonstrated that mutations of epidermal growth factor receptor (EGFR) were more frequent in females, whereas mutations in RBM10 were more common in male patients.…”

Section: Introductionmentioning

confidence: 99%

Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells

Guan

Tang

et al. 2017

Tumour Biol.

View full text Add to dashboard Cite

This study assessed RNA-binding motif 10 expression in lung adenocarcinoma tissues and examined the role and mechanism of RNA-binding motif 10 in the regulation of lung adenocarcinoma malignancy. Lung adenocarcinoma and corresponding adjacent non-tumor lung tissues from 41 patients were subjected to reverse transcription-polymerase chain reaction and Western blot assessment to detect RNA-binding motif 10 expression. Recombinant lentivirus carrying RNA-binding motif 10 complementary DNA was used to infect lung adenocarcinoma cell lines, A549 and H1299 cells. Complementary DNA microarray was used to profile RNA-binding motif 10-regulated genes. Levels of RNA-binding motif 10 messenger RNA and protein were significantly lower in lung adenocarcinoma tissues than those in paired nontumor tissues (p < 0.001). Reduced RNA-binding motif 10 expression was found to be associated with an advanced tumor stage. RNA-binding motif 10 overexpression inhibited viability and colony formation capacity of lung adenocarcinoma cell lines and induced cell-cycle arrest at G0/G1 phase in A549 cells and at S phase in H1299 cells. Complementary DNA microarray analysis identified 304 upregulated and 386 downregulated genes induced by RNA-binding motif 10 overexpression, which may be involved in cancer, focal adhesion, peroxisome proliferator-activated receptor-regulated gene pathway, cytokine-cytokine receptor interaction, mitogen-activated protein kinase signaling, complement and coagulation cascades, platelet amyloid precursor protein pathway, extracellular matrix-receptor interaction, and small cell lung cancer-related genes. Expression of FGF2, EGFR, WNT5A, NF-κB, and RAP1A was downregulated, whereas expression of AKT2, BIRC3, and JUN was upregulated. RNA-binding motif 10 messenger RNA and protein were reduced in lung adenocarcinoma tissues, and RNA-binding motif 10 overexpression inhibited lung adenocarcinoma cancer cell malignant behavior in vitro. Molecularly, RNA-binding motif 10 regulates many gene pathways involving in the tumor development or progression.

show abstract

Expression of RBM5‐related factors in primary breast tissue

Rintala-Maki

Goard

Langdon

et al. 2006

J of Cellular Biochemistry

View full text Add to dashboard Cite

The aim of this study was to examine the expression of the RBM5 tumor suppressor, in relation to RBM6 and RBM10, to obtain a better understanding of the potential role played by these RBM5-related factors in the regulation of RBM5 tumor-suppressor activity. Paired non-tumor and tumor samples were obtained from 73 breast cancer patients. RNA and protein expression were examined by semi-quantitative reverse transcription-polymerase chain reaction and immunoblot, respectively. Data were analyzed using various statistical methods to test for correlations amongst the RBM5-related factors, and between the factors and various pathological parameters. Most notably, RBM5, RBM10v1, and HER2 protein expression levels were elevated in tumor tissue (P < 0.0001). RBM5 and RBM10v1 protein expression were significantly positively correlated (P < 0.001), as were RBM5 and HER2 protein expression (P < 0.01), in both non-tumor and tumor tissue, whereas RBM10v1 and HER2 protein expression were only marginally correlated, in non-tumor tissue (P < 0.05). Interestingly, RBM5 and RBM10v1 protein expression were both deregulated in relation to RNA expression in tumor tissue. RBM10v2 and RBM6 RNA were highly significantly positively correlated in relation to various factors relating to poor prognosis (P < 0.0001). To our knowledge, this study is the first to examine RBM5 expression at both the RNA and protein level in primary breast tumor tissue, and the first to examine expression of all RBM5-related factors in a comprehensive manner. The results provide a graphic illustration that RBM5-related factors are significantly differentially expressed in breast cancer, and suggest complex inter-related regulatory networks involving alternative splicing, oncogenic expression, and tissue-specific function.

show abstract

Positive correlation between the expression of X‐chromosome RBM genes (RBMX, RBM3, RBM10) and the proapoptotic Bax gene in human breast cancer

Cited by 71 publications

References 13 publications

Nuclear expression of the RNA-binding protein RBM3 is associated with an improved clinical outcome in breast cancer

Nuclear expression of the RNA-binding protein RBM3 is associated with an improved clinical outcome in breast cancer

Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells

Expression of RBM5‐related factors in primary breast tissue

Contact Info

Product

Resources

About