Positive and Negative Selection of the T Cell Repertoire: Role of MHC and Other Ligands

Simpson, Elizabeth

doi:10.3109/08830189209053512

Cited by 5 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We wished to establish the CD2-myc mice (originally derived from C57B1/6 and CBA/Ca inbred strains) on a background that should result in the deletion of VPJl 1 cells. CBA/Ca mice carry Mtv loci 8 and 9 together with a functional I-E gene (Simpson, 1992), by backcrossing onto CBA/Ca mice it was predicted that the offspring would delete Vp11-expressing cells. To confirm that these F, mice could present endogenous superantigen a representative sample of the tumours was screened for the presence of an intact I-Ea gene (J Picard, personal communcation).…”

Section: Resultsmentioning

confidence: 99%

“…Analysis of a thymic lymphoma associated with a field case of feline leukaemia virus (FeLV) infection revealed the presence of a provirus which had incorporated a fully processed TCR gene, raising the possibility that antigen stimulation or inappropriate antigen recognition may have been important in the genesis of this tumour (Fulton et al, 1987). Studies involving murine radiation leukaemia virus (RadLV) have shown that free virus particles can modulate the growth of a RadLV-induced T-cell lymphoma cell line (O'Neill, 1994) and that the VP repertoire of RadLV-induced lymphomas is restricted, indicating non-random selection (Sen-Majumdar et al, 1994 Acha-Orbea & Palmer, 1991;Simpson,-1992;Marrack et al, 1993;Simpson et al, 1993 transferred to nylon filters and hybridised to the appropriate radiolabelled probes at high stringency using the protocols described by Sambrook et al (1989). Random priming (Random Priming Kit, Amersham) and radiolabelling of these probes was also carried out using methods described by Sambrook et al (1989).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Apparent bypass of negative selection in CD8+ tumours in CD2-myc transgenic mice

Cameron

Campbell²,

Blyth³

et al. 1996

Br J Cancer

View full text Add to dashboard Cite

Summary A role for antigen stimulation in lymphoid neoplasia has been postulated and is supported by indirect evidence that suggests that the interaction of antigen with both T cells and B cells may constitute an epigenetic event that can contribute to tumour induction or tumour progression. Using myc-bearing transgenic mice that develop mainly clonal T-cell lymphomas we have investigated the possibility that endogenous antigen-mediated clonal deletion might be overridden in tumorigenesis. CD2-myc transgenic mice were backcrossed on to a CBA/Ca background to ensure Mtv-mediated deletion of VP I 1-expressing T cells in the resultant offspring. Lymphomas arising from these mice were subsequently screened for VPl expression.There was a clear correlation between the age at which mice developed neoplasia and the tumour phenotype. Mice with CD4-CD8+ tumours succumbed to thymic lymphoma at a significantly younger age than mice developing CD4+ CD8+ tumours. A small number of tumours consisted of the 'forbidden' VPI I phenotype, showing that cells vulnerable to transformation could escape negative selection. The majority of the VBI 1-positive tumours were CD4-CD8+ and were only observed in mice showing clinical evidence of tumour development at a relatively young age. The phenotype of these cells and the age at which tumours arose suggests that T cells escaping tolerance may be susceptible to transformation.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Apparent bypass of negative selection in CD8+ tumours in CD2-myc transgenic mice

Cameron

Campbell²,

Blyth³

et al. 1996

Br J Cancer

View full text Add to dashboard Cite

show abstract

Lymphocytes

Hwang¹,

Actor²

2014

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Lymphocytes are white blood cells that are derived from a common lymphoid progenitor in the bone marrow. They function as specific mediators involved in inducible immune responses against infectious agents. The classically defined adaptive B and T lymphocytes use defined surface molecules, antigen receptors, to detect antigens. The activation of lymphocytes requires multiple signals, including binding of the antigen‐specific receptor to the antigen, costimulation and/or exposure to environmental molecules. Activation results in an expansion of clonally reactive cells, which become effector cells (plasma cells and activated T cells) that function to eliminate pathogens, or which become memory cells to facilitate later secondary responses. A secondary group of lymphocytes, the innate lymphocytes (natural killer (NK) cells, γδ T cells, invariant NK T cells and B‐1 cells) also play a role in immune function. Specifically, they represent a pool of cells that respond quickly to a limited repertoire of antigens at sites of infection. Key Concepts: Cells of the lymphoid lineage are derived from the common lymphoid progenitors that differentiate from multipotent haematopoietic stem cells in the bone marrow. The role of lymphocytes is to facilitate recognition of foreign antigens (immune stimulators) and to mount responses for their neutralization and/or elimination. The developmental stages of B and T cells correspond to gene rearrangement events, leading to the different specificity of B cell and T cell receptors. B cells mature in the bone marrow, whereas T cells undergo education and maturation in the thymus. B lymphocytes utilise an immunoglobulin receptor to recognise conformational antigens. Upon activation, they mature into plasma cells to secrete specific antibodies with directed target specificity. T lymphocytes recognised processed antigen presented via major histocompatibility molecules. Multiple phenotypic subsets exist, allowing for differential regulation of cellular immune function. T‐helper cells express CD4 molecules, and assist in both cellular and humoral functions. Specific helper activity is directed via secretion of pleiotropic cytokines. Cytotoxic T cells express CD8 molecules, and target viral infected or tumour targets for destruction. All T lymphocytes bear the CD3 molecule to assist in signalling once antigen is encountered. The primary response of lymphocytes leads to the generation of ‘daughter clones’, which become effectors, a small fraction of which become memory cells. This generates a persistent population that can rapidly expand and respond with higher magnitude upon secondary encounter with an antigen. The innate lymphocytes (natural killer cells, γδ T cells, invariant natural killer T cells and B‐1 cells) represent a special reservoir of cells that are able to respond quickly to a limited subset of antigens at sites of infection.

show abstract

Development of Efficient Prophylactic Vaccines and Theracines for Newborns and Infants

Bona¹

Neonatal Immunity

View full text Add to dashboard Cite

Positive and Negative Selection of the T Cell Repertoire: Role of MHC and Other Ligands

Cited by 5 publications

References 39 publications

Apparent bypass of negative selection in CD8+ tumours in CD2-myc transgenic mice

Apparent bypass of negative selection in CD8+ tumours in CD2-myc transgenic mice

Lymphocytes

Development of Efficient Prophylactic Vaccines and Theracines for Newborns and Infants

Contact Info

Product

Resources

About