Positional Specificities in Phospholipid Hydrolyses<sup>*</sup>

Robertson, Alex; Lands, William E.M.

doi:10.1021/bi00911a012

Cited by 235 publications

(47 citation statements)

References 24 publications

(22 reference statements)

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“…After snake venom hydrolysis, lysophosphatidyl choline was the only phospholipid evident on thin layer chromatography. It was assumed that the free fatty acids were derived from the beta position (14,15). The free fatty acids from the 2 Ross Allen Reptile Institute, Silver Springs, Fla. beta position in the solvent front were methylated, extracted, dried, and stored as described above.…”

Section: Methodsmentioning

confidence: 99%

Correlations of Mechanical Stability, Morphology, Pulmonary Surfactant, and Phospholipid Content in the Developing Lamb Lung*

Brumley¹,

Chernick²,

Hodson³

et al. 1967

J. Clin. Invest.

177

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Section: Methodsmentioning

confidence: 99%

Correlations of Mechanical Stability, Morphology, Pulmonary Surfactant, and Phospholipid Content in the Developing Lamb Lung*

Brumley¹,

Chernick²,

Hodson³

et al. 1967

J. Clin. Invest.

177

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“…The biosynthesis of the specifically labelled PC was carried out as described by Robertson & Lands (1962). The reaction flask contained 250,uCi of [1-'4C]-oleic acid (57 mCi/mmol), 2 mg carrier oleic acid and 20 mg LPC.…”

Section: Measurement Oforganicphosphorusmentioning

confidence: 99%

The Distribution and Metabolism of Arachidonic Acid in Rabbit Platelets During Aggregation and Its Modification by Drugs

Blackwell

Duncombe

Flower

et al. 1977

British J Pharmacology

258

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1Gas chromatographic and radio-isotope labelling techniques have been used to establish the origin of the arachidonic acid used by the platelet cyclo-oxygenase for the synthesis of pro-aggregatory prostaglandin endoperoxide derivatives. 2 Measurements of total platelet arachidonate content indicated that more than 95% is esterified in the phosphatide fraction of the cells. 3 During aggregation by collagen or thrombin as much as 80% of the total platelet arachidonate may be liberated and transformed by the platelet enzymes into hydroxyacids and other more polar compounds. 4 The phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol fractions are major sources of the arachidonate thus used. Indomethacin, which prevents platelet aggregation by inhibiting the cyclo-oxygenase, did not affect this release of arachidonate from the phosphatides but did prevent the transformation of arachidonate to endoperoxide derivatives. 6 Mepacrine, a drug which possesses weak anti-phospholipase activity in platelets, also prevents aggregation by collagen or thrombin, but seems to do so by preventing substrate release from the phosphatide fraction. 7 It is suggested that phospholipase A2 plays a key role in the initial events during platelet aggregation induced by collagen.

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“…Robertson and Lands (1962) and Ottolenghi (1967) observed a higher activity in the lung in comparison with that in the liver. The former was about three times of the latter.…”

Section: Discussionmentioning

confidence: 85%

“…Preliminary experiments were carried out according to the method of Robertson and Lands (1962). Table 1 shows the phospholipase A activity in lung and liver.…”

Section: Resultsmentioning

confidence: 99%

Phospholipase A Activity in Rat Lung

Ohta

Hasegawa

1972

Tohoku J. Exp. Med.

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Positional Specificities in Phospholipid Hydrolyses^*

Cited by 235 publications

References 24 publications

Correlations of Mechanical Stability, Morphology, Pulmonary Surfactant, and Phospholipid Content in the Developing Lamb Lung*

Correlations of Mechanical Stability, Morphology, Pulmonary Surfactant, and Phospholipid Content in the Developing Lamb Lung*

The Distribution and Metabolism of Arachidonic Acid in Rabbit Platelets During Aggregation and Its Modification by Drugs

Phospholipase A Activity in Rat Lung

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Positional Specificities in Phospholipid Hydrolyses*

Cited by 235 publications

References 24 publications

Correlations of Mechanical Stability, Morphology, Pulmonary Surfactant, and Phospholipid Content in the Developing Lamb Lung*

Correlations of Mechanical Stability, Morphology, Pulmonary Surfactant, and Phospholipid Content in the Developing Lamb Lung*

The Distribution and Metabolism of Arachidonic Acid in Rabbit Platelets During Aggregation and Its Modification by Drugs

Phospholipase A Activity in Rat Lung

Contact Info

Product

Resources

About

Positional Specificities in Phospholipid Hydrolyses^*