Positional cloning of the mouse retrovirus restriction gene Fvl

Best, Steve; Tissier, Paul Le; Towers, Greg J.; Stoye, Jonathan P.

doi:10.1038/382826a0

Cited by 451 publications

(355 citation statements)

References 29 publications

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“…Fv1 is a gene on distal chromosome 4, within the linkage region mapped in this study, that has been shown to influence retroviral replication (19). Thus, Fv1 is a good candidate gene to explain the linkage data in NZ mice.…”

Section: Investigation Of Fv1 As a Candidate Gene For The Chromosome mentioning

confidence: 99%

“…4 reverse). Additionally, the previously published polymorphic region (19) was sequenced in C57BL/10 and BALB/c mice. Briefly, PCR product was amplified with the forward primer in the presence of fluorescently labeled di-deoxynucleotides (Big-Dye terminators; Applied Biosystems, Warrington, U.K.) and purified, and the sequence was determined by capillary electrophoresis (3700 DNA Analyser; Applied Biosystems).…”

Section: Typing Of Friend Virus Susceptibility 1 (Fv1) Allelesmentioning

confidence: 99%

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A Novel Locus Regulates Both Retroviral Glycoprotein 70 and Anti-Glycoprotein 70 Antibody Production in New Zealand Mice When Crossed with BALB/c

Rigby

Rozzo

Gill

et al. 2004

The Journal of Immunology

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Lupus-prone New Zealand Black and New Zealand White mice produce high serum levels of the endogenous retroviral envelope protein gp70 and develop an Ab response to this autoantigen as part of their autoimmune disease. Linkage analysis of two crosses involving New Zealand and BALB/c mice mapped these traits to a group of overlapping loci, including a novel locus on proximal chromosome 12. This locus was linked with serum gp70 and the autoimmune response against it. The linkage of serum gp70 levels to a previously described locus on distal chromosome 4 was also confirmed. Sequence analysis of a candidate gene on distal chromosome 4, Fv1, provided support that this gene may be associated with the control of serum gp70 levels in both New Zealand Black and New Zealand White mice. Linkage data and statistical analysis confirmed a close correlation between gp70 Ag and anti-gp70 Ab levels, and together gave support to the concept that a threshold level of gp70 is required for the production of anti-gp70 Abs. Serum levels of anti-gp70 Abs were closely correlated with the presence of renal disease, more so than anti-dsDNA Abs. Understanding the genetic basis of this complex autoantigen-autoantibody system will provide insight into the pathogenesis of lupus in mice, which may have implications for human disease.

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Section: Investigation Of Fv1 As a Candidate Gene For The Chromosome mentioning

confidence: 99%

Section: Typing Of Friend Virus Susceptibility 1 (Fv1) Allelesmentioning

confidence: 99%

A Novel Locus Regulates Both Retroviral Glycoprotein 70 and Anti-Glycoprotein 70 Antibody Production in New Zealand Mice When Crossed with BALB/c

Rigby

Rozzo

Gill

et al. 2004

The Journal of Immunology

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“…One of the most well characterized restriction factors is the murine Fv1 gene, which prevents infection by particular strains of murine leukemia virus (MLV) (Best et al, 1996; for review, see Bieniasz, 2004;Goff, 2004). It is known that restriction by Fv1 involves the recognition of a capsid determinant in MLV (DesGroseillers et al, 1983;Kozak and Chakraborti, 1996).…”

Section: Introductionmentioning

confidence: 99%

TRIM5α Cytoplasmic Bodies Are Highly Dynamic Structures

Campbell

Dodding

Yap

et al. 2007

MBoC

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Tripartite motif (TRIM)5␣ has recently been identified as a host restriction factor that has the ability to block infection by certain retroviruses in a species-dependent manner. One interesting feature of this protein is that it is localized in distinct cytoplasmic clusters designated as cytoplasmic bodies. The potential role of these cytoplasmic bodies in TRIM5␣ function remains to be defined. By using fluorescent fusion proteins and live cell microscopy, we studied the localization and dynamics of TRIM5␣ cytoplasmic bodies. This analysis reveals that cytoplasmic bodies are highly mobile, exhibiting both short saltatory movements and unidirectional long-distance movements along the microtubule network. The morphology of the cytoplasmic bodies is also dynamic. Finally, photobleaching and photoactivation analysis reveals that the TRIM5␣ protein present in the cytoplasmic bodies is very dynamic, rapidly exchanging between cytoplasmic bodies and a more diffuse cytoplasmic population. Therefore, TRIM5␣ cytoplasmic bodies are dynamic structures more consistent with a role in function or regulation rather than protein aggregates or inclusion bodies that represent dead-end static structures. INTRODUCTIONThe species-specific tropism of many retroviruses is determined by cellular restriction factors present in the cells of the host. One of the most well characterized restriction factors is the murine Fv1 gene, which prevents infection by particular strains of murine leukemia virus (MLV) (Best et al., 1996; for review, see Bieniasz, 2004;Goff, 2004). It is known that restriction by Fv1 involves the recognition of a capsid determinant in MLV (DesGroseillers et al., 1983;Kozak and Chakraborti, 1996). Analysis of Fv1 restriction indicates that restricted MLV strains can generate reverse transcription products but that they are unable to complete subsequent steps in infection (Pryciak and Varmus, 1992) and also that this restriction of infection is saturable, because it can be overcome with high levels of virus (Pincus et al., 1975;Duran-Troise et al., 1977).A similar restriction to human immunodeficiency virus 1 (HIV-1) infection in primates has also been well characterized; and recently, the cellular factor present in the cells of Old World monkeys responsible for restricting infection by HIV-1 was cloned and identified as the cytoplasmic body component tripartite motif (TRIM)5␣ . Restriction of HIV-1 infection by TRIM5␣ resembles restriction of MLV infection by Fv1 in many ways. TRIM5␣-mediated restriction is saturable with high multiplicity of infection (MOI), and the specificity of TRIM5␣-mediated HIV-1 restriction is governed by the recognition of a capsid determinant in the restricted virus similar to the case of Fv1-mediated restriction of MLV infection (Towers et al., 2000;Cowan et al., 2002;Owens et al., 2003Owens et al., , 2004Hatziioannou et al., 2004). However, TRIM5␣-mediated restriction differs from Fv1-mediated restriction in that TRIM5␣-mediated restriction is manifested earlier in the infectious pathway, b...

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“…These cellular factors were first identified by Friend virus susceptibility gene 1 (Fv1), 85 a restriction factor in mouse cells that blocks specific strains of MLV. 86 Fv1 encodes a protein similar to mouse and human ERV-L endogenous retrovirus Gag 87,88 but the mechanism of Fv1 restriction remains unclear. MLV sensitivity to Fv1 restriction is determined by amino acid 110 in the viral CA protein.…”

Section: Avoiding Host Cell Restrictionsmentioning

confidence: 99%