Position Paper: Extravascular and Plasma Prorenin and Renin

Sealey, Jean E.; Atlas, Steven A.; Laragh, John H.

doi:10.1007/978-1-4612-5899-5_44

Cited by 2 publications

(1 citation statement)

References 36 publications

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“…Plasma inactive renin, which comprises 70-90% of total circulating renin and exhibits unique reversible activation when exposed to acid, has also been a focal point of much investigation (7)(8)(9)(10)(11). Recent identification (12,13) of plasma inactive renin as prorenin, a biosynthetic precursor, prompted us to attempt the production of human prorenin in quantities sufficient for its biochemical characterization and for clarification of the activation mechanisms.…”

mentioning

confidence: 99%

Synthesis and Characterization of Human Prorenin in Escherichia coli1

Imai¹,

Cho²,

Takamatsu³

et al. 1986

The Journal of Biochemistry

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DNA sequences encoding Ile-Glu-Gly-Arg and human prorenin were joined and placed under the transcriptional control of the Escherichia coli trp promoter-operator in the expression vector pTR501. E. coli cells transformed with pTR501 expressed high levels (30% of total cell protein) of prorenin as part of a hybrid protein with the trp E gene product. The chimeric protein, accumulated in a sedimentable form, was dissolved in 6 M guanidine X HCl, purified to near homogeneity, and renatured by dialysis. The complete prorenin sequence was then excised from the renatured hybrid protein using blood coagulation factor Xa, a proteinase which is highly specific for the tetrapeptide insert Ile-Glu-Gly-Arg introduced between the 9 amino-terminal residues of the trp E gene product and the first amino acid (Thr 1) of prorenin. Human prorenin thus obtained was readily activatable with trypsin and showed close similarities to naturally occurring prorenin in its biochemical and immunochemical properties.

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mentioning

confidence: 99%

Synthesis and Characterization of Human Prorenin in Escherichia coli1

Imai¹,

Cho²,

Takamatsu³

et al. 1986

The Journal of Biochemistry

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Renin–Angiotensin–Aldosterone System and the Renal Regulation of Sodium, Potassium, and Blood Pressure Homeostasis

Laragh

Sealey

1992

Comprehensive Physiology

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The sections in this article are: Historical Background Renin Aldosterone A Coordinated Renal‐Adrenal Hormonal System: Renin, Angiotensin, and Aldosterone Comparative Aspects Brief Overview of the Renin‐Angiotensin‐Aldosterone System Biochemistry and Molecular Biology of the Renin System Prorenin Renin Renin Substrate Angiotensins I and II Angiotensin‐Converting Enzyme Aldosterone Methods of Measurement Physiology Prorenin Renin Renin Substrate Angiotensin II Aldosterone Cybernetics of the System Coordinated Regulation of Sodium, Potassium, and Blood Pressure Use of the Renin System to Understand Blood Pressure Control and Disorders of Pressure‐Volume Homeostasis Two Forms of Vasoconstriction: Vasoconstriction‐Volume Analysis Three Basic Tenets Concerning Plasma Renin Measurements The Sodium—Calcium Connection Dynamic Reciprocity of Two Forms of Vasoconstriction The Sympathetic Nervous System and Modulation of the Two Forms of Arteriolar Vasoconstriction Human Disorders of the Renin System Hypertensive Disorders Renin System Abnormalities in Edematous States Hypokalemic Normotensive States Summary

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Position Paper: Extravascular and Plasma Prorenin and Renin

Cited by 2 publications

References 36 publications

Synthesis and Characterization of Human Prorenin in Escherichia coli1

Synthesis and Characterization of Human Prorenin in Escherichia coli1

Renin–Angiotensin–Aldosterone System and the Renal Regulation of Sodium, Potassium, and Blood Pressure Homeostasis

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