Pos0424 characterization of the Inflammatory Proteome of Synovial Fluid From Patients With Psoriatic Arthritis: Potential Treatment Targets

Abstract

BackgroundSynovial membrane inflammation, driven by effector T-cell activation and altered inflammatory cytokine expression, is a key feature of this psoriatic arthritis. The proximity extension assay (PEA) technique could contribute to identifying novel protein biomarkers associated with PsA and to improving the biological understanding and the future management of PsA, leading to novel intervention targets.Objectives1) To characterize the inflammatory proteome of synovial fluid (SF) from patients with Psoriatic Arthritis (PsA) using a next generation proteomics technique, and 2) to evaluate its potential to stratify patients according to clinical features.MethodsInflammatory proteome profile of SF from thirteen PsA patients with active knee arthritis were analysed using PEA technology (Olink Target 96 Inflammation panel, Cobiomic Biosciences). Four patients with OA were included as control group.ResultsSeventy-nine inflammation-related proteins were detected in SF from PsA patients (SF-PsA). Unsupervised analyses of the molecular proteome profile in SF-PsA identified two specific phenotypes characterized by higher or lower levels of inflammation-related proteins. Clinically, SF-PsA with higher levels of inflammatory proteins also showed increased systemic inflammation and altered glucose and lipid metabolisms. Besides, SF from PsA patients showed 39 out of 79 proteins significantly altered compared to SF-OA specifically related to cell migration and inflammatory response. Among these, molecules such as TNFα, IL-17A, IL-6, IL-10, IL-8, ENRAGE, CCL20, TNFSF-14, OSM, IFNγ, MCP-3, CXCL-11, MCP4, CASP-8, CXCL-6, CD-6, ADA, CXCL-10, TNFβ and IL-7 showed the most significantly alteration.ConclusionThis is the first study that characterizes the inflammatory landscape of synovial fluid of PsA patients by analyzing a panel of 92 inflammatory proteins using PEA technology. Novel SF proteins have been described as potential pathogenic molecules involved in the pathogenesis of PsA. Despite the flare, inflammatory proteome could distinguish two different phenotypes related to systemic inflammation and lipid and glucose alterations.Acknowledgements:Funded by ISCIII (PI20/00079 and RD21/0002/0033) co-financed by ERDF.REFERENCES:NIL.Acknowledgements:NIL.Disclosure of InterestsNone Declared.