Portulaca oleracea L. Polysaccharide Inhibits Porcine Rotavirus In Vitro

Zhou, Xiechen; Li, Yan; Li, Tao; Cao, Junyang; Guan, Zhongbin; Xu, Thomas T.; Jia, Guiyan; Ma, Guorong; Zhao, Rui

doi:10.3390/ani13142306

Cited by 3 publications

(4 citation statements)

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“…The genotype of rotavirus ZTR-68 is G1P [ 8 ], and the genotype of the SA11 strain is G3P [ 2 ]. They were isolated and preserved at the Molecular Biology Laboratory of the Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College.…”

Section: Methodsmentioning

confidence: 99%

“…The calculation formula was as follows: inhibition rate (%) = (A compound group − A virus group )/(A blank group − A virus group ) × 100%. In this experiment, a purified goat anti-rotavirus G1P [ 8 ] antibody (batch number: RVAB2020101), preserved by the Institute of Medical Biology, Chinese Academy of Medical Sciences, was used as the primary antibody in the ELISA experiment, and the secondary antibody was an HRP-labeled goat anti-mouse purified antibody (batch number: RVAB2020101H).…”

Section: Methodsmentioning

confidence: 99%

“…Deoxyshikonin can inhibit rotavirus replication by inducing low SIRT1, ac-Foxo1, Rab7, and VP6 protein levels, low RV titers, low autophagy, and oxidative stress [ 7 ]. The antiviral effect of Portulaca oleracea L. polysaccharide (POL-P), an active component of Portulaca oleracea L(POL), inhibits rotavirus replication by upregulating the expression of IFN-α [ 8 ]. Inhibitors of dihydroorotate dehydrogenase (the rate-limiting enzyme for de novo pyrimidine synthesis) (BQR) can resist rotavirus infection by inhibiting pyrimidine biosynthesis in cells and intestinal organoids [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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ML241 Antagonizes ERK 1/2 Activation and Inhibits Rotavirus Proliferation

Wang,

Hu,

et al. 2024

Viruses

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Rotavirus (RV) is the main pathogen that causes severe diarrhea in infants and children under 5 years of age. No specific antiviral therapies or licensed anti-rotavirus drugs are available. It is crucial to develop effective and low-toxicity anti-rotavirus small-molecule drugs that act on novel host targets. In this study, a new anti-rotavirus compound was selected by ELISA, and cell activity was detected from 453 small-molecule compounds. The anti-RV effects and underlying mechanisms of the screened compounds were explored. In vitro experimental results showed that the small-molecule compound ML241 has a good effect on inhibiting rotavirus proliferation and has low cytotoxicity during the virus adsorption, cell entry, and replication stages. In addition to its in vitro effects, ML241 also exerted anti-RV effects in a suckling mouse model. Transcriptome sequencing was performed after adding ML241 to cells infected with RV. The results showed that ML241 inhibited the phosphorylation of ERK1/2 in the MAPK signaling pathway, thereby inhibiting IκBα, activating the NF-κB signaling pathway, and playing an anti-RV role. These results provide an experimental basis for specific anti-RV small-molecule compounds or compound combinations, which is beneficial for the development of anti-RV drugs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ML241 Antagonizes ERK 1/2 Activation and Inhibits Rotavirus Proliferation

Wang,

Hu,

et al. 2024

Viruses

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“…However, there is no definite conclusion as to which compound plays a major role. Unlike other compounds, Portulaca oleracea L. polysaccharide (POL) is a polymeric carbohydrate composed of several monosaccharides, which has the advantages of low toxicity and high biological activity [ 22 ]. The anti-tumor effects of POL have been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Portulaca oleracea L. polysaccharide inhibits ovarian cancer via inducing ACSL4-dependent ferroptosis

Xia,

Yang,

Liu

2024

Aging

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The antitumor effect of Portulaca oleracea L. polysaccharide (POL) has been demonstrated, but whether it curbs the development of ovarian cancer has not been reported. Here, we treated ovarian cancer cells with different concentrations of POL, detected cell activity by CCK-8 assay, and apoptosis rate by flow cytometry. The results showed that SKOV3 and Hey cell survival decreased with increasing POL concentration in a dose-dependent manner. POL significantly inhibited ovarian cancer cell migration and increased cell death compared with the control group. Ferroptosis inhibitors, but not apoptosis, necrosis, and autophagy inhibitors, reversed POL-induced cell death. Further studies revealed that POL promoted the accumulation of lipid reactive oxygen species (ROS), Fe2+, malondialdehyde (MDA), and decreased glutathione (GSH) production. Moreover, POL significantly increased the mortality of ovarian cancer cells. In vivo studies confirmed that POL reduced the volume and weight of tumors and increased the levels of Fe2+ and MDA in mice in vivo . Western blot assay revealed that POL increased the expression of ACSL4 in ovarian cancer cells as well as in tumors in mice in vivo . More importantly, the POL-mediated increase in lipid ROS, Fe2+, MDA, and decrease in GSH were significantly reversed after knocking down ACSL4 in ovarian cancer cells. Thus, POL can effectively inhibit ovarian cancer development, which may be achieved by increasing ACSL4-mediated ferroptosis. These results suggest that POL has the potential to be a potential drug for targeted treatment of ovarian cancer.

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The protective role of prebiotics and probiotics on diarrhea and gut damage in the rotavirus-infected piglets

Yang,

Fan,

Mao

et al. 2024

J Animal Sci Biotechnol

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Rotavirus is one of the pathogenic causes that induce diarrhea in young animals, especially piglets, worldwide. However, nowadays, there is no specific drug available to treat the disease, and the related vaccines have no obvious efficiency in some countries. Via analyzing the pathogenesis of rotavirus, it inducing diarrhea is mainly due to disturb enteric nervous system, destroy gut mucosal integrity, induce intracellular electrolyte imbalance, and impair gut microbiota and immunity. Many studies have already proved that prebiotics and probiotics can mitigate the damage and diarrhea induced by rotavirus infection in hosts. Based on these, the current review summarizes and discusses the effects and mechanisms of prebiotics and probiotics on rotavirus-induced diarrhea in piglets. This information will highlight the basis for the swine production utilization of prebiotics and probiotics in the prevention or treatment of rotavirus infection in the future.

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Portulaca oleracea L. Polysaccharide Inhibits Porcine Rotavirus In Vitro

Cited by 3 publications

References 50 publications

ML241 Antagonizes ERK 1/2 Activation and Inhibits Rotavirus Proliferation

ML241 Antagonizes ERK 1/2 Activation and Inhibits Rotavirus Proliferation

Portulaca oleracea L. polysaccharide inhibits ovarian cancer via inducing ACSL4-dependent ferroptosis

The protective role of prebiotics and probiotics on diarrhea and gut damage in the rotavirus-infected piglets

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