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Baukloh, Anja C.

doi:10.1007/978-3-322-86687-5_16

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…The aim of tissue processing is to facilitate sperm search, and both mechanical and enzymatic techniques yield similar results (28,29). After processing, epididymal and testicular spermatozoa can be either used for ICSI or cryopreserved.…”

Section: Laboratory Processing Of Surgi-cally-retrieved Spermmentioning

confidence: 99%

Sperm retrieval techniques for assisted reproduction

Esteves¹,

Miyaoka²,

Agarwal³

2011

Int. braz j urol.

126

123

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Different surgical methods such as PESA, MESA, TESA, TESE and micro-TESE have been developed to retrieve spermatozoa from either the epididymis or the testis according to the type of azoospermia, i.e., obstructive or non-obstructive. Laboratory techniques are used to remove contaminants, cellular debris, and red blood cells following collection of the epididymal fluid or testicular tissue. Surgically-retrieved spermatozoa may be used for intracytoplasmic sperm injection (ICSI) and/or cryopreservation. In this article, we review the surgical procedures for retrieving spermatozoa from both the epididymis and the testicle and provide technical details of the commonly used methods. A critical analysis of the advantages and limitations of the current surgical methods to retrieve sperm from males with obstructive and non-obstructive azoospermia is presented along with an overview of the laboratory techniques routinely used to process surgically-retrieved sperm. Lastly, we summarize the results from the current literature of sperm retrieval, as well as the clinical outcome of ICSI in the clinical scenario of obstructive and nonobstructive azoospermia.

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Section: Laboratory Processing Of Surgi-cally-retrieved Spermmentioning

confidence: 99%

Sperm retrieval techniques for assisted reproduction

Esteves¹,

Miyaoka²,

Agarwal³

2011

Int. braz j urol.

126

123

View full text Add to dashboard Cite

show abstract

“…The specimens were assessed under an inverted microscope at 3300 for the presence of spermatozoa. 20…”

Section: Surgical Technique Of Testicular Sperm Extraction (Tese)mentioning

confidence: 99%

The number of spermatozoa collected with testicular sperm extraction is a novel predictor of intracytoplasmic sperm injection outcome in non-obstructive azoospermic patients

Cavallini¹,

Magli²,

Crippa³

et al. 2011

Asian J Androl

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The purpose of this study was to determine the relationships between monitors of spermatogenesis and predictors of the intracytoplasmic sperm injection (ICSI) outcome in patients with non-obstructive azoospermia (NOA) undergoing testicular sperm extraction (TESE). Seventy-nine patients with NOA (mean age: 43.6±5.2 years), each of whom yielded (97 000±3040) spermatozoa with conventional TESE, were considered in our analysis. Their partners (mean age: 35.8±5.1 years) underwent a total of 184 ICSI cycles; 632 oocytes were collected, 221 oocytes were injected, 141 oocytes were fertilized, 121 embryos were obtained, 110 embryos were transferred, 14 clinical pregnancies were achieved and only one miscarriage occurred. Multivariate regression analysis indicated relationships between the percentage of fertilized oocytes, transferred embryos and clinical pregnancies with the following variable values: female partner's age, number of spermatozoa collected, testicular volume, male partner's levels of follicle stimulating hormone (FSH), number of oocytes collected, number of oocytes injected and number of ICSI cycles. A significant inverse relationship was found between female partner's age or male partner's FSH levels and biochemical pregnancies. A significant direct relationship emerged between the number of ICSI cycles and the percentage of oocytes fertilized, embryos transferred and biochemical pregnancies, and between the number of spermatozoa collected per testicular biopsy and biochemical pregnancies. The number of spermatozoa was positively linked to the number of clinical pregnancies, independent of the number of ICSI cycles and the number of oocytes collected/injected. The number of spermatozoa collected, FSH level and testicular volume are monitors of spermatogenesis linked to ICSI success.

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Portugal

Gröner¹

2012

Verbände Und Interessengruppen in Den Ländern Der Europäischen Union

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Portugal

Cited by 3 publications

References 8 publications

Sperm retrieval techniques for assisted reproduction

Sperm retrieval techniques for assisted reproduction

The number of spermatozoa collected with testicular sperm extraction is a novel predictor of intracytoplasmic sperm injection outcome in non-obstructive azoospermic patients

Portugal

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