Portasomes as Coupling Factors in Active Ion Transport and Oxidative Phosphorylation

Harvey, William R.; Cioffi, Moira; Wolfersberger, Michael G.

doi:10.1093/icb/21.3.775

Cited by 77 publications

(22 citation statements)

References 33 publications

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“…In longitudinal paraffin sections the H + V-ATPase antibody labeling paralleled published reports of portasomes (Harvey et al, 1981), which are V 1 -ATPase particles (Grüber et al, 2000). Portasomes have been reported in certain microvilli of other mosquito species (Clements, 1992;Volkmann and Peters, 1989;Zhuang et al, 1999).…”

Section: H + V-atpase Labeling Of Portasome-containing Membranessupporting

confidence: 62%

“…aegypti (Patrick et al, 2006) and in An. gambiae reported here underscores the long-neglected fact that the cytoplasmic sector of the enzyme V 1 -ATPase can be seen in electron micrographs where they have been called 'portasomes' (Harvey, 1980;Harvey et al, 1981). They were first described as a 'coat of repeating subunits that stud the cytoplasmic surface of the apical plasma membranes in blowfly rectal papillae' (Gupta and Berridge, 1966); soon after, similar subunits were reported on the apical membranes of caterpillar midgut goblet cells (Anderson and Harvey, 1966).…”

Section: -Atpases In Midgut Epithelial Cells Are Portasomesmentioning

confidence: 55%

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Cationic pathway of pH regulation in larvae ofAnopheles gambiae

Okech

Boudko

Linser

et al. 2008

Journal of Experimental Biology

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Section: H + V-atpase Labeling Of Portasome-containing Membranessupporting

confidence: 62%

Section: -Atpases In Midgut Epithelial Cells Are Portasomesmentioning

confidence: 55%

Cationic pathway of pH regulation in larvae ofAnopheles gambiae

Okech

Boudko

Linser

et al. 2008

Journal of Experimental Biology

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“…Since the discovery that the V-ATPase constituted the apical portasomes present at high abundance in insect epithelia (Berridge and Oschman, 1969;Harvey et al, 1983;Harvey et al, 1981;Schweikl et al, 1989), and the demonstration that it was indeed functioning as a proton-motive ATPase (Schweikl et al, 1989;Wieczorek et al, 1991), it has been necessary to infer one or more co-localized alkali-metal cation/proton exchangers (termed by this In this case, the search was for ʻurate oxidaseʼ. Even though this dataset is based on highly technical microarray data, it is easy to deduce that this is effectively a tubule-specific gene.…”

Section: The Elusive Apical ʻWieczorekʼ Exchangermentioning

confidence: 99%

Insights into the Malpighian tubule from functional genomics

Dow

2009

Journal of Experimental Biology

121

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SummaryClassical physiological study of the Malpighian tubule has led to a detailed understanding of fluid transport and its control across several species. With the sequencing of the Drosophila genome, and the concurrent development of post-genomic technologies such as microarrays, proteomics, metabolomics and systems biology, completely unexpected roles for the insect Malpighian tubule have emerged. As the insect body plan is simpler than that of mammals, tasks analogous to those performed by multiple mammalian organ systems must be shared out among insect tissues. As well as the classical roles in osmoregulation, the Malpighian tubule is highly specialized for organic solute transport, and for metabolism and detoxification. In Drosophila, the adult Malpighian tubule is the key tissue for defence against insecticides such as DDT; and it can also detect and mount an autonomous defence against bacterial invasion. While it is vital to continue to set insights obtained in Drosophila into the context of work in other species, the combination of post-genomic technologies and physiological validation can provide insights that might not otherwise have been apparent for many years.

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“…The most accessible and spectacular example of this transport could be found in the larval lepidopteran midgut, which can develop lumenpositive potentials in excess of 100 mV, drive currents of over 1 mA/cm 2 (96,98,100), and in some species generate a luminal pH in excess of 12, the highest in biology (58,70). Within the midgut, the apical membrane of a specialized goblet cell was decorated with an array of 10-nm spheres (5), termed "portasomes" (97), and it was on this membrane that active K ϩ transport was shown to take place (67). However, painstaking biochemical purification of the goblet cell apical membrane of lepidopteran midgut, based on purification of the portasomes (47), identified not a P-type ATPase similar to K ϩ -H ϩ -ATPases of vertebrates, but a V-type H ϩ -ATPase (205,246).…”

Section: Physiology and Molecular Genetics Of Ion Transport In mentioning

confidence: 99%

Integrative Physiology and Functional Genomics of Epithelial Function in a Genetic Model Organism

Dow

Davies

2003

Physiological Reviews

114

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Dow, Julian A. T, and Shireen A. Davies. Integrative Physiology and Functional Genomics of Epithelial Function in a Genetic Model Organism. Physiol Rev 83: 687–729, 2003; 10.1152/physrev.00035.2002.—Classically, biologists try to understand their complex systems by simplifying them to a level where the problem is tractable, typically moving from whole animal and organ-level biology to the immensely powerful “cellular” and “molecular” approaches. However, the limitations of this reductionist approach are becoming apparent, leading to calls for a new, “integrative” physiology. Rather than use the term as a rallying cry for classical organismal physiology, we have defined it as the study of how gene products integrate into the function of whole tissues and intact organisms. From this viewpoint, the convergence between integrative physiology and functional genomics becomes clear; both seek to understand gene function in an organismal context, and both draw heavily on transgenics and genetics in genetic models to achieve their goal. This convergence between historically divergent fields provides powerful leverage to those physiologists who can phrase their research questions in a particular way. In particular, the use of appropriate genetic model organisms provides a wealth of technologies (of which microarrays and knock-outs are but two) that allow a new precision in physiological analysis. We illustrate this approach with an epithelial model system, the Malpighian (renal) tubule of Drosophila melanogaster. With the use of the beautiful genetic tools and extensive genomic resources characteristic of this genetic model, it has been possible to gain unique insights into the structure, function, and control of epithelia.

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Portasomes as Coupling Factors in Active Ion Transport and Oxidative Phosphorylation

Cited by 77 publications

References 33 publications

Cationic pathway of pH regulation in larvae ofAnopheles gambiae

Cationic pathway of pH regulation in larvae ofAnopheles gambiae

Insights into the Malpighian tubule from functional genomics

Integrative Physiology and Functional Genomics of Epithelial Function in a Genetic Model Organism

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