Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood

Liu, Yibiao; Liu, Xingyun; Li, Mifang; Liu, Qiong; Xu, Tailin

doi:10.3390/bios12080564

Cited by 17 publications

(9 citation statements)

References 38 publications

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“…DPV peak current decreased after the modification of antibodies, thereby indicating successful antibody immobilization on the electrode surface. The antibody concentration used in the experiment is 10 μg/mL according to our previous study [ 30 , 32 ]. Subsequently, BSA was employed to obstruct unbound sites, resulting in a further reduction of the DPV peak current ( Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

Vertical Graphene-Based Printed Electrochemical Biosensor for Simultaneous Detection of Four Alzheimer’s Disease Blood Biomarkers

Huang

et al. 2023

Biosensors

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Early detection and timely intervention play a vital role in the effective management of Alzheimer’s disease. Currently, the diagnostic accuracy for Alzheimer’s disease based on a single blood biomarker is relatively low, and the combined use of multiple blood biomarkers can greatly improve diagnostic accuracy. Herein, we report a printed electrochemical biosensor based on vertical graphene (VG) modified with gold nanoparticles (VG@nanoAu) for the simultaneous detection of four Alzheimer’s disease blood biomarkers. The printed electrochemical electrode array was constructed by laser etching and inkjet printing. Then gold nanoparticles were modified onto the working electrode surface via electrodeposition to further improve the sensitivity of the sensor. In addition, the entire printed electrochemical sensing system incorporates an electrochemical micro-workstation and a smartphone. The customized electrochemical micro-workstation incorporates four electro-chemical control chips, enabling the sensor to simultaneously analyze four biomarkers. Consequently, the printed electrochemical sensing system exhibits excellent analytical performance due to the large surface area, biocompatibility, and good conductivity of VG@nanoAu. The detection limit of the sensing system for Aβ40, Aβ42, T-tau, and P-tau181 was 0.072, 0.089, 0.071, and 0.051 pg/mL, respectively, which meets the detection requirements of Alzheimer’s disease blood biomarkers. The printed electrochemical sensing system also exhibits good specificity and stability. This work has great value and promising prospects for early Alzheimer’s disease diagnosis using blood biomarkers.

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Section: Resultsmentioning

confidence: 99%

Vertical Graphene-Based Printed Electrochemical Biosensor for Simultaneous Detection of Four Alzheimer’s Disease Blood Biomarkers

Huang

et al. 2023

Biosensors

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“… Technique Sensing platform Sample Linear range LOD Refs. EIS Ab/Protein G/3,3'-dithiobis(sulfosuccinimidyl propionate) (DTSSP)/Gold Electrode BSA & Human serum 0.01 pM to 10 nM 0.03 pM 63 DPV Aptamer-Au-CS bioconjugate /BSA/Ab/MPA/Gold Electrode Human serum 0.5 pM to 100 pM 0.42 pM 64 EIS 3-aminophenol MIP/C-SPE PBS 2.18 pM to 2.18 nM 0.024 pM 65 EIS n-butylamine/Ab/lipoic acid/Au Electrode PBS – 100 μg/mL 66 DPV carbon nanocomposite film composed of MWCNTs, rGO, and CS/Gold Electrode Human serum 0.5–80 fM 0.46 fM 67 DPV Apt/AuNPs/Paper-based vertical graphene electrode (VGE) Blood 0.1 pg/mL to 1 ng/mL 0.034 pg/mL 68 SWV, CV Ab/MWCNTs-PAH/Pt/C-SPE Serum 8.6–1100 pg/mL 0.24 pg/mL 69 Amperometric HRP-DAb/tau/capture antibody/AuNPs-PAMAM/C-SPE Undiluted human plasma and brain tissue extracts 11–800 pg mL …”

Section: Resultsmentioning

confidence: 99%

Application of Pt@ZIF-8 nanocomposite-based electrochemical biosensor for sensitive diagnosis of tau protein in Alzheimer’s disease patients

Chakari-Khiavi,

Mirzaie,

Khalilzadeh

et al. 2023

Sci Rep

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Alzheimer’s disease (AD) is a progressive brain disorder characterized by the ongoing decline of brain functions. Studies have revealed the detrimental effects of hyperphosphorylated tau (p-tau) protein fibrils in AD pathogenesis, highlighting the importance of this factor in the early-stage detection of AD conditions. We designed an electrochemical immunosensor for quantitative detection of the cis conformation of the p-tau protein (cis-p-tau) employing platinum nanoparticles (Pt NPs) supported on zeolitic imidazolate frameworks (ZIF) for modifying the glassy carbon electrode (GCE) surface. Under optimum conditions, the immunosensor selectively and sensitively detected cis-p-tau within the broad linear range of 1 fg mL−1 to 10 ng mL−1 and the low limit of detection (LOD) of 1 fg mL−1 with desired reproducibility and stability. Furthermore, the fabricated immunosensor's performance was examined for the cis-p-tau analysis in the serum of AD patients, indicating its accuracy and feasibility for real-sample analysis. Notably, this is the first application of Pt@ZIF-8 nanocomposite in fabricating a valid immunosensor for selective cis-p-tau detection, even in the presence of trans-p-tau. It is worth mentioning that the enzyme-linked immunosorbent assay (ELISA) reference technique is not able to evaluate pico- or femtomolar concentrations of cis-p-tau, making the fabricated immunosensor superior for early-stage measurement and screening of AD.

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“…The vertical structure also provided good performance in graphene-based electrochemical sensors, where the resistance between the electrode and electrolyte decreases as the surface area increases [97]. In general, the vertical structure of graphene is also fabricated using a similar CVD technique [97,98].…”

Section: Surface Propertiesmentioning

confidence: 99%

Two‐Dimensional Materials Integrated in Electrochemical Biosensors for Virus Detection: Property Engineering Strategies and Device Applications

Wulandari,

Septiani,

Gumilar

et al. 2024

ChemBioEng Reviews

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Electrochemical biosensors have been widely used as advanced methods for virus detection. Ongoing efforts are being made to improve their performance by investigating novel materials as electrode modifiers. In this regard, two‐dimensional (2D) materials, such as graphene, transition metal dichalcogenides (TMDs), and transition metal carbides/nitrides (MXenes), have been extensively explored due to their exceptional structural and electrical properties for electrochemical biosensors. This review investigates and summarizes the recently developed 2D materials employed in virus detection. Various strategies to enhance the critical properties of 2D materials, including conductivity, surface area, active sites, selectivity, and wettability, on electrochemical biosensing platforms, which impact their performance, are discussed. Different types of 2D materials (pristine, derivatives, and composites) are described and subsequently evaluated regarding their applications in electrochemical biosensing. This review serves as a valuable guide for designing next‐generation, high‐performance electrochemical biosensors based on 2D materials.

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Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood

Cited by 17 publications

References 38 publications

Vertical Graphene-Based Printed Electrochemical Biosensor for Simultaneous Detection of Four Alzheimer’s Disease Blood Biomarkers

Vertical Graphene-Based Printed Electrochemical Biosensor for Simultaneous Detection of Four Alzheimer’s Disease Blood Biomarkers

Application of Pt@ZIF-8 nanocomposite-based electrochemical biosensor for sensitive diagnosis of tau protein in Alzheimer’s disease patients

Two‐Dimensional Materials Integrated in Electrochemical Biosensors for Virus Detection: Property Engineering Strategies and Device Applications

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