Portable real-time PCR system using tablet-based fluorescence imaging

Bialek, Hayden; Dawes, Jacob; Heer, Donald; Johnston, Matthew L.

doi:10.1109/embsisc.2016.7508599

Cited by 4 publications

(5 citation statements)

References 6 publications

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“…Some innovative and portable real-time PCR detection devices have been proposed in prior research, but they still exhibit some shortcomings. For instance, integration is relatively low, and only the detection function of a single fluorescence detection channel is specified [ 31 ]; gradient amplification analysis for multiple samples and melt curve analysis are not performed [ 25 ]. Our system’s amplification detection speed is comparable to commercial real-time PCR devices, which is one of its shortcomings.…”

Section: Resultsmentioning

confidence: 99%

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Hundreds-Dollar-Level Multiplex Integrated RT-qPCR Quantitative System for Field Detection

et al. 2022

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The COVID-19 pandemic poses a threat to global health. Due to its high sensitivity, specificity, and stability, real-time fluorescence quantitative (real-time PCR) detection has become the most extensively used approach for diagnosing SARS-CoV-2 pneumonia. According to a report from the World Health Organization, emerging and underdeveloped nations lack nucleic acid detection kits and polymerase chain reaction (PCR) instruments for molecular biological detection. In addition, sending samples to a laboratory for testing may result in considerable delays between sampling and diagnosis, which is not favorable to the timely prevention and control of new crown outbreaks. Concurrently, there is an urgent demand for accurate PCR devices that do not require a laboratory setting, are more portable, and are capable of completing testing on-site. Hence, we report on HDLRT-qPCR, a new, low-cost, multiplexed real-time fluorescence detection apparatus that we have developed for on-site testing investigations of diverse diseases in developing nations. This apparatus can complete on-site testing rapidly and sensitively. The entire cost of this instrument does not exceed USD 760. In order to demonstrate the applicability of our PCR instrument, we conducted testing that revealed that we achieved gradient amplification and melting curves comparable to those of commercially available equipment. Good consistency characterized the testing outcomes. The successful detection of target genes demonstrates the reliability of our inexpensive PCR diagnostic technique. With this apparatus, there is no need to transport samples to a central laboratory; instead, we conduct testing at the sampling site. This saves time on transportation, substantially accelerates overall testing speed, and provides results within 40 min.

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Section: Resultsmentioning

confidence: 99%

“…However, because of their sensitivity and signal reporting methods, real-time PCR is a technology that is ten times more expensive than standard PCR platforms [ 6 , 7 , 8 , 9 ]. Therefore, it is of great significance to develop a low-cost, real-time quantitative PCR instrument [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Hundreds-Dollar-Level Multiplex Integrated RT-qPCR Quantitative System for Field Detection

et al. 2022

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“…The qPCR solved the pivotal problems in the conventional PCR; however, the cost was usually approximately 10 times compared to the one of conventional PCR. For example, commercial PCR instruments, such as the Applied Biosystems (ABI) PRISMfi 7900HT, Bio-Rad iQ5 systems, and so forth, have been used extensively with greater detection performance, but their cost is expensive, their volume is bulky, and their operation is complex. , Based on this fact, we developed a novel PCR system with advantages of low cost, small size, as well as user-friendly software system. The system includes mainly a hardware control circuit system and a software function system.…”

Section: Introductionmentioning

confidence: 99%

Construction of Very Low-Cost Loop Polymerase Chain Reaction System Based on Proportional-Integral-Derivative Temperature Control Optimization Algorithm and Its Application in Gene Detection

et al. 2022

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Real-time polymerase chain reaction (PCR) technology is essential in nucleic acid detection and point-of-care testing (POCT). However, nowadays, the classical qPCR instrument has the deficiency of its bulky volume, high cost, and inconvenience to use; hence, a low-cost and easy-to-use PCR equipment was thus developed consisting of a hardware subsystem as well as a software subsystem based on an improved proportional-integral-derivative (PID) system. The proposed system not only could hold self-setting reaction cycles of temperature rising and falling automatically but also the temperature during the constant temperature stage was regulated steady based on improved temperature control algorithm, which proved its great effect compared with the reaction temperature derived from an infrared thermal imaging camera. The experimental results in gene detection research also could indicate its applicability and stability of our developed PCR system by using the amplification curve analysis, the melting curve analysis, and agarose gel electrophoresis analysis compared with the commercial PCR instrument, which illustrates the great potential application value of the proposed PCR system.

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“…It is a mature technology, and many commercial instruments have been produced. Representative companies that Bio-Rad (Hercules, CA, USA), ABI (Applied Biosystems, New York, NY, USA), Biosynex (Alsace, France), ThermoFisher (Waltham, MA, USA) built a battery-powered, real-time fluorescent PCR instrument using TEC heating and cooling [23]. The TEC is simple and easy to integrate but is time-consuming and has a high power consumption.…”

Section: Introductionmentioning

confidence: 99%

“…The TEC is simple and easy to integrate but is time-consuming and has a high power consumption. Typically, 30 cycles consume a power of 6 Ah [23].…”

Section: Introductionmentioning

confidence: 99%

Battery-Powered Portable Rotary Real-Time Fluorescent qPCR with Low Energy Consumption, Low Cost, and High Throughput

Sang

2020

Biosensors

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The traditional qPCR instrument is bulky, expensive, and inconvenient to carry, so we report a portable rotary real-time fluorescent PCR (polymerase chain reaction) that completes the PCR amplification of DNA in the field, and the reaction can be observed in real-time. Through the analysis of a target gene, namely pGEM-3Zf (+), the gradient amplification and melting curves are compared to commercial devices. The results confirm the stability of our device. This is the first use of a mechanical rotary structure to achieve gradient amplification curves and melting curves comparable to commercial instruments. The average power consumption of our system is about 7.6 W, which is the lowest energy consumption for real-time fluorescence quantification in shunting PCR and enables the use of our device in the field thanks to its self-contained power supply based on a lithium battery. In addition, all of the equipment costs only about 710 dollars, which is far lower than the cost of a commercial PCR instrument because the control system through mechanical displacement replaces the traditional TEC (thermoelectric cooler) temperature control. Moreover, the equipment has a low technical barrier, which can suit the needs of non-professional settings, with strong repeatability.

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Portable real-time PCR system using tablet-based fluorescence imaging

Cited by 4 publications

References 6 publications

Hundreds-Dollar-Level Multiplex Integrated RT-qPCR Quantitative System for Field Detection

Hundreds-Dollar-Level Multiplex Integrated RT-qPCR Quantitative System for Field Detection

Construction of Very Low-Cost Loop Polymerase Chain Reaction System Based on Proportional-Integral-Derivative Temperature Control Optimization Algorithm and Its Application in Gene Detection

Battery-Powered Portable Rotary Real-Time Fluorescent qPCR with Low Energy Consumption, Low Cost, and High Throughput

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