Portable, Quantitative Detection of <i>Bacillus</i> Bacterial Spores Using Surface-Enhanced Raman Scattering

Cowcher, David P.; Xu, Yun; Goodacre, Royston

doi:10.1021/ac303657k

Cited by 139 publications

(113 citation statements)

References 24 publications

(42 reference statements)

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“…It is difficult to study the bacterium in quantity since all Raman spectra only reveal the vibration information about the molecules at the substrate surface [91]. Cowcher David et al developed a nanoparticle-based SERS system to detect the bacteria expressed small molecule for the bacteria identification [92]. By incubating the cells with nanoparticles, the nanoparticles will diffuse into the cell and accumulate in the lysosomes [93].…”

Section: Discussionmentioning

confidence: 99%

“…However, not like nanoparticle systems, the solid-based SERS substrate could not get into the cell as free as nanoparticles. In most applications, the solid-based SERS was focused the organic molecule [32,44,45,81] and bacteria identification [94][95][96][97]. Yamazoe et al first applied a gold nanocoral structure which is solid-based transparent boehmite for visualizing the ischemic core of mouse brain without labeling and pretreatment of the tissue [98].…”

Section: Discussionmentioning

confidence: 99%

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Optical integrated chips with micro and nanostructures for refractive index and SERS-based optical label-free sensing

et al. 2015

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Label-free optical biosensing technologies have superior abilities of quantitative analysis, unmodified targets, and ultrasmall sample volume, compared to conventional fluorescence-label-based sensing techniques, in detecting various biomolecules. In this review article, we introduce our recent results in the field of evanescent-wavebased refractive index sensing and surface enhanced Raman scattering (SERS)-based sensing, both of which are promising platforms for label-free optical biosensors. First, silicon-on-insulator (SOI) nanowire waveguide and metallic surface plasmon resonance (SPR)-based refractive index sensing are discussed. In order to improve the detection limit, phase interrogation techniques are introduced to these types of sensors based on prism-coupled SPR and SOI microring resonators. A detection limit in the order of 10 −6 refractive index unit is achieved. Detection of 16.7 pM anti-IgG is also demonstrated based on the SPR devices. Second, SERS substrates based on various nanometallic structures are discussed. Metallic nanowire arrays and inverted nanopyramids and grooves with a thin metal surface are fabricated based on anisotropic wetetching of silicon substrates. Both structures have demonstrated a Raman signal enhancement on the order of 10 7 .In order to improve the extraction efficiency of the Raman signal at a high wave number, a nano-bowtie array substrate is fabricated, which exhibits double resonances at both the excitation wavelength and the desired Raman scattering wavelength. Experimental results have shown that this double-resonance structure can further enhance the received Raman signal, as compared to conventional SERS substrates with only one resonance at the excitation wavelength.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Optical integrated chips with micro and nanostructures for refractive index and SERS-based optical label-free sensing

et al. 2015

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“…The colloid was assessed using UV-visible spectroscopy and scanning electron microscopy (SEM) and was very similar to that used in previously pub-lished studies. 25,26,34,35 Instrumentation Initial experiments were carried out to generate conventional Raman spectra of powdered codeine and codeine-d6, which were placed on a calcium fluoride (CaF2) disc and Raman spectra were recorded using 633 nm excitation (see ESI † for more details of the conventional Raman measurements). SERS spectra were recorded using a DeltaNu Advantage portable Raman spectrometer (DeltaNu, Laramie, WY, USA), equipped with a 633 nm HeNe laser emitting ∼3 mW on to the sample.…”

Section: Synthesis Of Silver Nanoparticlesmentioning

confidence: 99%

Quantitative detection of codeine in human plasma using surface-enhanced Raman scattering via adaptation of the isotopic labelling principle

Subaihi

Muhamadali

Mutter

et al. 2017

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aIn this study surface enhanced Raman scattering (SERS) combined with the isotopic labelling (IL) principle has been used for the quantification of codeine spiked into both water and human plasma. Multivariate statistical approaches were employed for the analysis of these SERS spectral data, particularly partial least squares regression (PLSR) which was used to generate models using the full SERS spectral data for quantification of codeine with, and without, an internal isotopic labelled standard. The PLSR models provided accurate codeine quantification in water and human plasma with high prediction accuracy (Q 2 ). In addition, the employment of codeine-d6 as the internal standard further improved the accuracy of the model, by increasing the Q 2 from 0.89 to 0.94 and decreasing the low root-mean-square error of predictions (RMSEP) from 11.36 to 8.44. Using the peak area at 1281 cm −1 assigned to C-N stretching, C-H wagging and ring breathing, the limit of detection was calculated in both water and human plasma to be 0.7 µM (209.55 ng mL −1) and 1.39 µM (416.12 ng mL −1 ), respectively. Due to a lack of definitive codeine vibrational assignments, density functional theory (DFT) calculations have also been used to assign the spectral bands with their corresponding vibrational modes, which were in excellent agreement with our experimental Raman and SERS findings. Thus, we have successfully demonstrated the application of SERS with isotope labelling for the absolute quantification of codeine in human plasma for the first time with a high degree of accuracy and reproducibility. The use of the IL principle which employs an isotopolog (that is to say, a molecule which is only diff erent by the substitution of atoms by isotopes) improves quantifi-cation and reproducibility because the competition of the codeine and codeine-d6 for the metal surface used for SERS is equal and this will off set any diff erence in the number of particles under analysis or any fluctuations in laser fluence. It is our belief that this may open up new exciting opportunities for testing SERS in real-world samples and applications which would be an area of potential future studies.

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“…Hand-held SERS systems 55 have been demonstrated for intraoperative detection of malignant tumors, 60 detection of foodcontamination-related molecules, 61 and sensing bacteria with nanomolar sensitivity. 62 The combination of a gold-nanoparticle SERS platform with an exponential amplifi cation reaction enabled detection of microRNAs in lung cancer cells with a sensitivity to 0.5 fM. 63 This technique therefore provided a six-order-of-magnitude improvement in sensitivity compared to existing SERS-based direct assays and a nine-order-ofmagnitude improvement compared to northern blot methods for microRNA detection.…”

Section: Biosensingmentioning

confidence: 99%

Localized fields, global impact: Industrial applications of resonant plasmonic materials

et al. 2015

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Portable, Quantitative Detection of Bacillus Bacterial Spores Using Surface-Enhanced Raman Scattering

Cited by 139 publications

References 24 publications

Optical integrated chips with micro and nanostructures for refractive index and SERS-based optical label-free sensing

Optical integrated chips with micro and nanostructures for refractive index and SERS-based optical label-free sensing

Quantitative detection of codeine in human plasma using surface-enhanced Raman scattering via adaptation of the isotopic labelling principle

Localized fields, global impact: Industrial applications of resonant plasmonic materials

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