Portable Incubator and Its Use in Insecticide Bioassays with Field Populations of Lygus Bugs, Aphids, and Other Insects1

Brindley, William A.; Al-Rajhi, Deifalla H.; Rose, Randy L.

doi:10.1093/jee/75.4.758

Cited by 7 publications

(3 citation statements)

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“…In this regard, immersion techniques may deserve further attention. Although often dismissed as unrealistic or imprecise (results cannot be expressed in terms of toxicant per gram of body weight), immersion tests ensure uniform contact and often give steep concentration mortality curves, with slopes of approximately 4 to 10 on a probit scale (e.g., Hemingway et al 1984, Halliday andGeorghiou 1985), and lower standard deviations than residual or topical tests (e.g., Brindley et al 1982). Immersion of field-collected lepidopterous larvae in dilute solutions of formulated insecticide has proved to be quick and practical for monitoring resistance in areas where laboratory facilities are unavailable, and it may have potential for use on other insects, such as aphids (Watkinson et al 1984).…”

Section: Choice Of Exposure Methodsmentioning

confidence: 99%

“…Such methods should therefore be thoroughly tested before extensive use. Controlling temperature during pesticide exposure may also be a potential problem, but techniques are available to standardize temperature, even in the field (Brindley et al 1982). However, biochemical methods outlined in the following section offer the potential advantage of more rapid and reproducible assays.…”

Section: Standardizationmentioning

confidence: 99%

“…However, this problem can be overcome by adjusting incubation times at different temperatures (Beach et al 1989) or through field incubators (Brindley et al 1982).…”

Section: Type Of Biochemical Assaymentioning

confidence: 99%

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Resistance Detection and Documentation: The Relative Roles of Pesticidal and Biochemical Assays

ffrench‐Constant¹,

Roush²

1990

Pesticide Resistance in Arthropods

133

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Section: Choice Of Exposure Methodsmentioning

confidence: 99%

Section: Standardizationmentioning

confidence: 99%

See 1 more Smart Citation

Resistance Detection and Documentation: The Relative Roles of Pesticidal and Biochemical Assays

ffrench‐Constant¹,

Roush²

1990

Pesticide Resistance in Arthropods

133

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Insect Resistance to Insecticides

Brown¹

2002

Encyclopedia of Agrochemicals

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Resistance to pesticides has evolved, not only in insects, but also in acarines, weeds, fungi, bacteria, rodents, and other pests. The phenomenon of resistance is due to Darwinian evolution at a rate that is accelerated by the intensity of selection from pesticides. Insects present many difficult challenges related to resistance in that they are especially well adapted for dispersal through flight, they have a high reproductive capacity with multiple generations per year, and they are often difficult to detect in products traded around the world, such as grain, flowers, or even used tires.

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Esterase isozymes in Lygus hesperus: Characterization and relationship with organophosphate resistance

Brindley

1994

Pestic. Sci.

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Esterases of Lygus hesperus Knight from five field and one laboratory populations were separated by non-denaturing polyacrylamide gel electrophoresis and were characterized for inhibition and substrate specificity. There were six zones of esterase activity toward naphthyl ester substrates. Significant inter-and intra-populational polymorphisms were observed. All the isozymes were carboxylesterases that were sensitive to inhibition by paraoxon and insensitive to eserine or EDTA (ethylenedinitrilotetraacetic acid). Carboxylesterases from L. hesperus more readily hydrolysed naphthyl esters with short side chains. Molecular weight estimation by non-denaturing polyacrylamide gel electrophoresis revealed that Est-1, the specific band of older females and eggs, contained two enzymes with relative molecular masses of 140000 and 112000. The four esterase allozymes in Est-3 had an average relative molecular mass of approximately 106000. The frequency of one putative allozyme in Est-3 was directly correlated with the LC,, values of trichlorfon (to which L. hesperus has become resistant). The developmental and distribution variability of the esterase isozymes are compared. Pestrc. Sci.

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Portable Incubator and Its Use in Insecticide Bioassays with Field Populations of Lygus Bugs, Aphids, and Other Insects1

Cited by 7 publications

References 0 publications

Resistance Detection and Documentation: The Relative Roles of Pesticidal and Biochemical Assays

Resistance Detection and Documentation: The Relative Roles of Pesticidal and Biochemical Assays

Insect Resistance to Insecticides

Esterase isozymes in Lygus hesperus: Characterization and relationship with organophosphate resistance

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