Portable electrochemical blood uric acid meter

Kuo, Cheng–Deng; Hwu, Chii‐Min; Lin, Yueh-Hui; Huang, Ya-Hsueh; Kao, Wei-Yi; Weih, Mei-Jy; Hsiao, Liang‐Tsai; Kwok, Ching Fai; Ho, ­Low‐Tone

doi:10.1002/jcla.10030

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…The available at‐home UA test kits like HumaSens plus and UASure require fingertip punctures, which hinders frequent UA measurement and discomforts patients‐ specifically children and elderly patients raising the need for a painless and convenient UA test [52–54] . Further, the at‐home UA test kits like UASure, cannot detect UA concentrations lower than 170 μM limiting their application for the diagnosis of certain diseases associated with low UA levels [53, 55] . Importantly, 100 % of the SUA can also be found in ISF, qualifying MN‐based detection as a highly promising method [56, 57] .…”

Section: Resultsmentioning

confidence: 99%

“…The elevated SUA level acts as a key risk factor predicting diabetic‐associated complications like stroke, kidney deterioration, and fatty liver disease, [50, 51] necessitating SUA monitoring in diabetic patients in addition to glucose. The available at‐home UA test kits like HumaSens plus and UASure require fingertip punctures, which hinders frequent UA measurement and discomforts patients‐ specifically children and elderly patients raising the need for a painless and convenient UA test [52–54] . Further, the at‐home UA test kits like UASure, cannot detect UA concentrations lower than 170 μM limiting their application for the diagnosis of certain diseases associated with low UA levels [53, 55] .…”

Section: Resultsmentioning

confidence: 99%

“…[52][53][54] Further, the at-home UA test kits like UASure, cannot detect UA concentrations lower than 170 μM limiting their application for the diagnosis of certain diseases associated with low UA levels. [53,55] Importantly, 100 % of the SUA can also be found in ISF, qualifying MN-based detection as a highly promising method. [56,57] We, thus, employed our HMN-GO.NA patches to identify the levels of UA and glucose in diabetic rats.…”

Section: Methodsmentioning

confidence: 99%

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A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

et al. 2023

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Point‐of‐care testing (POCT) of clinical biomarkers is critical to health monitoring and timely treatment, yet biosensing assays capable of detecting biomarkers without the need for costly external equipment and reagents are limited. Blood‐based assays are, specifically, challenging as blood collection is invasive and follow‐upprocessing is required. Here, we report a versatile assay that employs hydrogel microneedles (HMNs) to extract interstitial fluid (ISF), in a minimally invasive manner integrated with graphene oxide‐nucleic acid (GO.NA)‐based fluorescence biosensor to sense the biomarkers of interest in situ. The HMN‐GO.NA assay is supplemented with a portable detector, enabling a complete POCT procedure. Our system could successfully measure four clinically important biomarkers (glucose, uric acid (UA), insulin, and serotonin) ex vivo, in addition, to accurately detecting glucose and UA in vivo.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

et al. 2023

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“…Presisi alat ini juga dilaporkan cukup baik, dengan variabilitas yang rendah. Namun hasil pengukuran dengan sampel darah vena menunjukkan hasil yang lebih baik daripada hasil pengukuran dengan sampel darah kapiler (Kuo, 2001).…”

Section: Hasil Dan Pembahasanunclassified

Perbandingan Validitas Pemeriksaan Asam Urat Menggunakan Tiga Uric Acid Meter Dengan Baku Emas Standar Laboratorium

Fia

Rumawas

Martin

2022

JMSTKIK

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Uric acid is an end-product from Purine metabolism, excreted in kidney. Hyperuricemia is resulted from either over production or decreased excretion of uric acid. Hyperuricemia results in uric acid crystallization or deposition in plasm and extracellular fluid. Deposition of uric acid crystal in synovial fluid cause arthritis, called gouty arthritis. In Indonesia, the prevalence of arthritis (including gouty arthritis) is 11,9%. Monitoring uric acid level is important for hyperurisemic patients, as an increased of acute episodes of gouty arthritis corresponds to the raised of uric acid level. Standard uric acid determination should be done in laboratory, but in daily practice, patient come to primary health center for monitoring, and uric acid meter is used to determine uric acid level. Prior studies reported that uric acid meters could be used in daily practice, yet in Indonesia, there has no published study comparing the validity of different brands of uric acid meter available in the market. This analytic cross-sectional study aimed to determine the validity of three uric acid meters (UA sure, Autocheck dan Easytouch) as compared to the gold standard Laboratory method. Data of 39 adult men and women who were selected consecutively were analyzed by using the Bland-Altman method, one-sample t-test and ordinary least-square regression. Result of this study showed that the validity of UA sure and Easytouch uric acid meters were suitably good, whereas the validity of Autocheck was unrealiable due to the present of fixed bias dan proportional bias. Keywords: uric acid, hyperuricemia, gouty arthritis, uric acid meter Abstrak Asam urat adalah hasil akhir metabolisme purin yang diekskresikan melalui ginjal. Produksi berlebihan atau penurunan ekskresi, atau keduanya dapat menyebabkan hiperurisemia. Kadar asam urat yang berlebihan dapat menyebabkan kristalisasi dan penumpukan di plasma dan cairan ekstraseluler terutama di sendi sehingga mengakibatkan terjadinya radang sendi, yaitu artitis gout. Di Indonesia, prevalensi radang sendi, termasuk artritis gout sebesar 11,9%. Pada pasien hiperurisemia, pemantauan kadar asam urat sangat penting, karena angka kejadian artritis gout meningkat sesuai dengan tingginya asam urat plasma. Pemeriksaan asam urat standar dilakukan di laboratorium, namun pada prakteknya skrining kadar asam urat untuk pasien yang datang di layanan kesehatan primer sering dilakukan dengan menggunakan alat uric acid meter. Beberapa penelitian yang membandingkan hasil pemeriksaan asam urat dari laboratorium dengan uric acid meter melaporkan bahwa alat-alat tersebut dapat digunakan, namun di Indonesia belum ada penelitian yang membandingkan akurasi beberapa alat yang tersedia di pasaran. Penelitian ini merupakan penelitian analitik dengan desain potong lintang yang bertujuan untuk menilai validitas tiga alat uric acid meter (UA sure, Autocheck dan Easytouch) dengan baku emas standar laboratorium. Data kadar asam urat alat uric acid meter diperoleh dari sampel darah kapiler dan data kadar asam urat baku emas diambil dari sampel darah vena pada subyek yang sama dan waktu yang sama kemudian diukur dengan menggunakan metode enzymatic calorimetry di laboratorium standar. Data kemudian dianalisa dengan metode one-sample t-test dan ordinary least-square regression untuk membandingkan apakah terdapat bias pemeriksaan dari alat uric acid meter dengan baku emas. Pada penelitian ini didapatkan 39 sampel dengan hasil didapatkan UA sure dan Easy touch tidak mempunyai fixed bias (p=0,31; p=0,12) maupun proportional bias (p=0,91, p=0,42) terhadap baku emas, sedangkan Autocheck mempunyai fixed bias (p=0,001) dan proportional bias (p=0,006) bila dibandingkan dengan baku emas. Dari penelitian ini dapat disimpulkan UA sure dan Easy touch mempunyai validitas yang cukup baik, sedangkan Autocheck mempunyai validitas yang rendah.

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“…Alat ukur asam urat memiliki akurasi dan presisi yang baik dan terdapat korelasi linear dengan pengukuran metode standar (Kuo et al 2002) dalam pengukuran kadar asam urat dalam darah. Akurasi dan presisi alat dalam mengukur kadar asam urat dalam medium in vitro dibuktikan dengan hasil regresi dari kurva kalibrasi Gambar 1.…”

Section: Hasil Dan Pembahasanunclassified

Pengkajian Antihiperurisemia Emulsi Minyak Hati Ikan Kod Pada Mencit Swiss Webster Jantan

2017

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ABSTRAKEmulsi minyak hati ikan kod dipercaya membantu mencegah pembentukan asam urat. Penelitian ini bertujuan mengkaji aktivitas antihiperurisemia secara in vivo dan mengembangkan metode antihiperurisemia secara in vitro emulsi minyak hati ikan kod. Metode in vivo dilakukan menggunakan mencit Swiss Webster jantan yang diinduksi makanan tinggi purin 100 mg/kg BB suspensi hati ayam, bersama pemberian sediaan pembanding atau sediaan uji selama 20 hari. Kelompok kontrol sakit menerima induksi. Kelompok pembanding alopurinol menerima induksi dan 26 mg/kg BB alopurinol. Kelompok pembanding vitamin A menerima induksi dan 650 IU/kg BB vitamin A. Kelompok uji dosis rendah, sedang, dan tinggi menerima induksi dan 150 mg/kg BB, 300 mg/kg BB, dan 450 mg/kg BB emulsi minyak hati ikan kod. Efek antihiperurisemia terbesar ditunjukkan dosis 150 mg/kg BB dengan penurunan kadar asam urat sebesar 57,04% dibanding kelompok kontrol sakit. Hasil percobaan in vivo menunjukkan emulsi minyak hati ikan kod yang diberikan bersamaan induksi makanan tinggi purin memiliki efek antihiperurisemia. Metode in vitro dilakukan menggunakan asam urat dan basa purin murni yang ditambahkan homogenat hati tikus sebagai sumber enzim xantin oksidase. Hasil percobaan in vitro menunjukkan emulsi minyak hati ikan kod menguraikan asam urat dalam 0,525 mL medium campuran 0,075 mg asam urat dan 0,011 mL emulsi minyak hati ikan kod; emulsi minyak hati ikan kod mencegah pembentukan asam urat dari purin dalam 0,725 mL medium campuran 0,200 mg purin, 0,066 gram homogenat hati, dan 0,016 mL emulsi minyak hati ikan kod.Kata kunci : antihiperurisemia, emulsi minyak hati ikan kod, asam urat, purin. ABSTRACTCod liver oil emulsion is believed of being able to prevent uric acid accumulation. This research was held to examine antihyperuricemic activity with in vivo and develop antihyperuricemic method with in vitro of cod liver oil emulsion. In vivo method was accomplished using Swiss Webster male mice inducted with high purine meal 100 mg/kg BW chicken liver suspense, together with drugs or cod liver oil emulsion through 20 days. Control group consumed high purine meal. Allopurinol group consumed high purin meal and 26 mg/kg BW of allopurinol. Vitamin A group consumed high purine meal and 650 IU/kg BW of vitamin A. Low dose, medium dose, and high dose group consumed high purine meal and 150 mg/kg BW, 300 mg/kg BW, and 450 mg/kg BW of cod liver oil emulsion. Strongest antihyperuricemic capability was shown by dose of 150 mg/kg BW with 57,04% degradation of uric acid compared to control group. In vivo showed that cod liver oil emulsion given together with high purine meal had antihyperuricemic effect. In vitro method was accomplished using pure uric acid and purine base with rat liver homogenate as xanthine oxidase enzyme source added. In vitro showed cod liver oil emulsion degraded uric acid in 0,525 mL medium mixture of 0,075 mg of uric acid and 0,011 mL cod liver oil emulsion; cod liver oil emulsion prevented uric acid establishment from purine in 0,725 mL medium mixture of 0,200 mg of purin, 0,066 gram of liver homogenate, and 0,016 mL of cod liver oil emulsion. Keywords : antihyperuricemic, cod liver oil emulsion, uric acid, purine.

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Portable electrochemical blood uric acid meter

Cited by 12 publications

References 15 publications

A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

A Hydrogel Microneedle Assay Combined with Nucleic Acid Probes for On‐Site Detection of Small Molecules and Proteins

Perbandingan Validitas Pemeriksaan Asam Urat Menggunakan Tiga Uric Acid Meter Dengan Baku Emas Standar Laboratorium

Pengkajian Antihiperurisemia Emulsi Minyak Hati Ikan Kod Pada Mencit Swiss Webster Jantan

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