Portable and quantitative monitoring of heavy metal ions using DNAzyme-capped mesoporous silica nanoparticles with a glucometer readout

A mini-review on functional nucleic acids-based heavy metal ion detection

“…Introduction of Pb 2+ cleaved the substrate strands of the formed DNAzymes, triggered the gate open and resulted in release of the glucose from the pores. By detecting the released glucose molecules in the resulting solution, Pb 2+ at concentrations as low as 1.0 pM could be determined (Fu et al, 2013). In another design (Fig.…”

Amplified electrochemical sensing of lead ion based on DNA-mediated self-assembly-catalyzed polymerization

“…Catalytic strand (S 0 ): 5′-HS-TTTCATCTCTTCTCCGAGCCGGTC-GAAATAGTGAGT-3′ Substrate strand (S 1 ): 5′-ACTCACTATrAGGAAGAGATG -3′ Hybridization strand (S 2 ): 5′-TGGGTTGGGCGGGATGGGA-CAGCAGCTGACTCACTATT -3′ Hybridization strand (S 3 ): 5′-CAGCTGCTGTAA-TAGTGAGTGGGTAGGGCGGGTTGGGT -3′ Pb 2 þ -dependent DNAzyme comprising of catalytic strand (S 0 ) and substrate strand (S 1 ) was obtained by using the SELEX process as the reference (Fu et al, 2013). DNA stock solution was obtained by dissolving the oligonucleotides in Tris-HCl buffer (pH 7.4).…”

Low-cost and highly efficient DNA biosensor for heavy metal ion using specific DNAzyme-modified microplate and portable glucometer-based detection mode

“…Glucometer, one of the most widely used diagnostic devices, has utilized as a portable tool for the biochemical analyses owing to its easy operation, low cost and reliable quantitative results (Cha et al, 2014;Yan et al, 2013). Recently, our group designed several glucometer-based detection methods for quantitative monitoring of adenosine triphosphate, brevetoxin B and lead ion (Fu et al, 2013;Gao et al, 2014;Hou et al, 2014). To this end, our motivation is to construct a low-cost and highly efficient DNA biosensor for screening lead ion in the environmental sample using DNAzyme-modified microplate and portable glucometer-based detection mode.…”