Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells

Kato, Hirohito; Taguchi, Yoichiro; Tominaga, Kazuhiro; Umeda, Makoto; Tanaka, Akio

doi:10.1016/j.archoralbio.2013.11.008

Cited by 179 publications

(186 citation statements)

References 49 publications

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“…1 Lipopolysaccharide is one of the most abundant virulence factors from P. gingivalis, as it activates the host inflammatory response and disrupts bone remodeling process. 1, 18 Since the effect of PgLPS on PDLSCs remains unknown, the aim of the present study was to investigate whether this virulence factor would affect the biological properties of PDL progenitor cells. PDL heterogeneous cell population was purified to CD105 surface marker, the most frequently reported positive surface marker in MSCs.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of the active proliferation stage was also found in studies that assessed the effect of P. gingivalis endotoxin in pre-osteoblastic, 22 PDL, 23 and PDLSC cells. 18 Cytokines' gene expression was then performed to determine PgLPS effect on cell inflammatory response. Two pro-inflammatory cytokines that affect bone formation, 24 TNF-α and IL-1β, were investigated.…”

Section: Discussionmentioning

confidence: 99%

“…15,33,34 Cell lineage is another important factor; among the cited studies, only one investigated the effects of P. gingivalis LPS on PDLSCs. 18 However, this population was not isolated and highly CD105 + purified periodontal ligament progenitor cells were employed. Since it was already demonstrated that MSCs from PDL harbors a heterogeneous stem-cell-enriched population, 32,36,37 it is possible that PDLSCs composed by CD105 + cell subsets are less susceptible to P. gingivalis LPS challenge compared to heterogeneous PDLSC populations.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

Albiero¹,

Amorim²,

Casati³

et al. 2017

Braz. oral res.

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Periodontitis develops as a result of a continuous interaction between host cells and subgingival pathogenic bacteria. The periodontium has a limited capacity for regeneration, probably due to changes in periodontal ligament stem cells (PDLSCs) phenotype. The aim of this study was to evaluate the effects of lipopolysaccharides from Porphyromonas gingivalis (PgLPS) on mesenchymal phenotype and osteoblast/cementoblast (O/C) potential of PDLSCs. PDLSCs were assessed for Toll-like receptor 2 (TLR2) expression by immunostaining technique. After, cells were exposed to PgLPS, and the following assays were carried out: (i) cell metabolic activity using MTS; (ii) gene expression for IL-1β, TNF-α and OCT-4 by real-time polymerase chain reaction (RT-qPCR); (iii) flow cytometry for STRO-1 and CD105, and (iv) osteogenic differentiation. PDLSCs were positive for TLR2. PgLPS promoted cell proliferation, produced IL-1β and TNF-α, and did not affect the expression of stem cell markers, STRO-1, CD105 and OCT-4. Under osteogenic condition, PDLSCs exposed to PgLPS showed a similar potential to differentiate toward osteoblast/cementoblast phenotype compared to control group as revealed by mineralized matrix deposition and levels of transcripts for RUNX2, ALP and OCN. These results provide evidence that PgLPS induces pro-inflammatory cytokines, but does not change the mesenchymal phenotype and osteoblast/cementoblast differentiation potential of PDLSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

Albiero¹,

Amorim²,

Casati³

et al. 2017

Braz. oral res.

View full text Add to dashboard Cite

show abstract

“…33 According to our results, the amount of EC calcium deposition (Alizarin Red staining) after infection was higher on surfaces coated with PA. However, the Alizarin Red staining is used as a quality rather than a quantity biochemical mineralization assay; 34 therefore, gene expression was included in our study to control mineralization on the molecular level. The results of gene expression indicated that RG-I pectins prevent the decrease in osteoblast differentiation and matrix mineralization in response to P. gingivalis.…”

mentioning

confidence: 99%

Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following <em>Porphyromonas gingivalis</em> infection

Meresta¹,

Folkert²,

Gaber³

et al. 2017

IJN

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“…AGEs have been considered to induce inflammatory reaction in osteoblasts. We reported that hard tissue differentiation from mesenchymal stem cells decreases in inflammatory cond itions caused by Porphyromonas gingivalis LPS 23) . We therefore suggest that the hard tissue differentiation response decreases in conditions of inflammation, particularly increased expression of IL-6.…”

Section: Discussionmentioning

confidence: 95%

Effects of High Glucose for Hard Tissue Formation on Type II Diabetes Model Rat Bone Marrow Cells In Vitro

Okuda

Taguchi

Takahashi

et al. 2015

J. Hard Tissue Biology.

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Diabetes mellitus (DM) is one of the main etiologies and risk factors for periodontal disease, and is an important concern in periodontal medicine. It has been reported that high glucose concentrations mediate proliferation, differentiation and production of inflammatory cytokines on mesenchymal cells, but there are few reports on periodontal regenerative therapy in type II DM patients. The aim of this study was to examine the biological effects of high glucose conditions on GK rat (type II model) bone marrow mesenchymal cells (GK rat BMMSC). Cell culture experiments were performed with GK rat BMMSC. The effects of glucose at four concentrations (5.5, 8, 12 and 24 mM) were determined by examining cell proliferation, differentiation and production of inflammatory cytokines; the latter three concentrations are higher than the normal physiological glucose concentration, represented by 5.5 mM. High concentrations of glucose promoted proliferation and inhibited hard tissue differentiation and calcification of GK rat BMMSC. Differentiation correlated inversely with the expression of inflammatory cytokines, represented by IL-6. Our data suggest that high extracellular glucose concentrations promote proliferation and inhibit hard tissue differentiation and calcification in periodontal regeneration by causing an inflammatory response dependent on cytokines including IL-6.

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Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells

Cited by 179 publications

References 49 publications

Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following <em>Porphyromonas gingivalis</em> infection

Effects of High Glucose for Hard Tissue Formation on Type II Diabetes Model Rat Bone Marrow Cells In Vitro

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