Porphyromonas: A neglected potential key genus in human microbiomes

Guilloux, Charles-Antoine; Lamoureux, Claudie; Héry-Arnaud, Geneviève

doi:10.1016/j.anaerobe.2020.102230

Cited by 32 publications

(19 citation statements)

References 86 publications

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“…The genus Gemella is widely considered as a commensal of the respiratory niche, however, there is evidence for it being a potential opportunistic pathogen in older adults 53 . Similarly the genus Porphyromonas , more abundant in acute-ILI compared to controls, has been associated with pro-inflammatory responses 54 , 55 . Notably, the gut microbiota of individuals with acute-ILI was previously reported to harbor a pro-inflammatory profile 17 .…”

Section: Discussionmentioning

confidence: 99%

Associations and recovery dynamics of the nasopharyngeal microbiota during influenza-like illness in the aging population

Shetty

Beek

Bijvank

et al. 2022

Sci Rep

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Influenza-like illness (ILI), a disease caused by respiratory pathogens including influenza virus, is a major health concern in older adults. There is little information on changes and recovery dynamics of the nasopharyngeal (NP) microbiota of older adults associated with an ILI. Here, we compared the NP microbiota in older adults reporting (n = 240) or not (n = 157) ILI during the 2014–2015 influenza season at different times of the ILI event. A small but significant effect of the ILI was observed on the microbiota community composition and structure when compared to controls and samples collected at recovery. Corynebacterium was negatively associated with ILI and its abundance increased after recovery. Potential pathobionts such as Haemophilus, Porphyromonas and Gemella had higher abundances during acute-ILI. Stability and changes in the NP microbial community showed individual dynamics. Key core genera, Corynebacterium, Moraxella and Dolosigranulum exhibited higher inter-individual variability in acute-ILI, but showed comparable variability to controls after recovery. Participants in the ILI group with higher core microbiota abundances at the acute phase showed higher microbiota stability after recovery. Our findings demonstrate that acute-ILI is associated with alterations in the phylogenetic structure of the NP microbiota in older adults. The variation in the core microbiota suggests imbalances in the ecosystem, which could potentially play a role in the susceptibility and recovery of the NP microbiota after an ILI event.

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Section: Discussionmentioning

confidence: 99%

Associations and recovery dynamics of the nasopharyngeal microbiota during influenza-like illness in the aging population

Shetty

Beek

Bijvank

et al. 2022

Sci Rep

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“…Similarly, in 6 AP samples in the transient area, the average relative abundance of CT8 is 6.49% while in the remaining 39 AP samples it is only 0.37%. Members of CT8 P. pasteri / P. catoniae belong to the so-called POTG ( Porphyromonas other than gingivalis ) group of microorganisms ( Guilloux et al., 2020 ). Typically, they colonize lungs and lower airways and in some diseases like cystic fibrosis, the relative abundance of P. catoniae can serve as a marker to discriminate between various states of health ( Cuthbertson et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

The Oral Microbiome in Periodontal Health

Lenartova

Tesinska

Janatová

et al. 2021

Front. Cell. Infect. Microbiol.

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The estimation of oral microbiome (OM) taxonomic composition in periodontally healthy individuals can often be biased because the clinically periodontally healthy subjects for evaluation can already experience dysbiosis. Usually, they are included just based on the absence of clinical signs of periodontitis. Additionally, the age of subjects is used to be higher to correspond well with tested groups of patients with chronic periodontitis, a disorder typically associated with aging. However, the dysbiosis of the OM precedes the clinical signs of the disease by many months or even years. The absence of periodontal pockets thus does not necessarily mean also good periodontal health and the obtained image of “healthy OM” can be distorted.To overcome this bias, we taxonomically characterized the OM in almost a hundred young students of dentistry with precise oral hygiene and no signs of periodontal disease. We compared the results with the OM composition of older periodontally healthy individuals and also a group of patients with severe periodontitis (aggressive periodontitis according to former classification system). The clustering analysis revealed not only two compact clearly separated clusters corresponding to each state of health, but also a group of samples forming an overlap between both well-pronounced states. Additionally, in the cluster of periodontally healthy samples, few outliers with atypical OM and two major stomatotypes could be distinguished, differing in the prevalence and relative abundance of two main bacterial genera: Streptococcus and Veillonella. We hypothesize that the two stomatotypes could represent the microbial succession from periodontal health to starting dysbiosis. The old and young periodontally healthy subjects do not cluster separately but a trend of the OM in older subjects to periodontitis is visible. Several bacterial genera were identified to be typically more abundant in older periodontally healthy subjects.

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“…As discussed above, different results in microbiota characterization can be attributed mostly to individual and environmenmtal factors, but also to differences in methodologies, population size, reference databases, analytical platforms and assignment tools. Porphyromonas is an anaerobic bacterium normally present on the skin and ear of humans and dogs [7,12,21,50]. Although its presence is mostly associated with healthy mucosae, some species have been implicated in respiratory, mucosal, oral and skin infections [51,52].…”

Section: Plos Onementioning

confidence: 99%

Cerumen microbial community shifts between healthy and otitis affected dogs

et al. 2020

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Otitis externa is a common multifactorial disease in dogs, characterized by broad and complex modifications of the ear microbiota. The goal of our study was to describe the ear cerumen microbiota of healthy dogs, within the same animal and between different animals, and to compare the cerumen microbiota of otitis affected dogs with that of healthy animals. The present study included 26 healthy dogs, 16 animals affected by bilateral otitis externa and 4 animals affected by monolateral otitis externa. For each animal cerumen samples from the right and left ear were separately collected with sterile swabs, and processed for DNA extraction and PCR amplification of the 16S rRNA gene. Amplicon libraries were sequenced using an Ion Torrent Personal Genome Machine (PGM), and taxonomical assignment and clustering were performed using QIIME 2 software. Our results indicate that the bacterial community of the cerumen in healthy dogs was characterized by extensive variability, with the most abundant phyla represented by Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes and Fusobacteria. The analysis of both alpha and beta diversity between pairs of left and right ear samples from the same dog within the group of affected animals displayed higher differences than between paired samples across healthy dogs. Moreover we observed reduced bacterial richness in the affected group as compared with controls and increased variability in population structure within otitis affected animals, often associated with the proliferation of a single bacterial taxon over the others. Moreover, Staphylococcus and Pseudomonas resulted to be the bacterial genera responsible for most distances between the two groups, in association with differences in the bacterial community structure. The cerumen microbiota in healthy dogs exhibits a complex bacterial population which undergoes significant modifications in otitis affected animals.

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Porphyromonas: A neglected potential key genus in human microbiomes

Cited by 32 publications

References 86 publications

Associations and recovery dynamics of the nasopharyngeal microbiota during influenza-like illness in the aging population

Associations and recovery dynamics of the nasopharyngeal microbiota during influenza-like illness in the aging population

The Oral Microbiome in Periodontal Health

Cerumen microbial community shifts between healthy and otitis affected dogs

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