Porphyrin metabolism in some malignant diseases

El-Sharabasy, M. M. H.; El-Waseef, Ahmed; Hafez, M.; Salim, Sherman

doi:10.1038/bjc.1992.83

Cited by 143 publications

(84 citation statements)

References 13 publications

(3 reference statements)

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“…Differing PpIX accumulation between normal and tumor human colonic cells has been found to be influenced by (but not solely determined by) differences in porphobilinogen deaminase (production of PpIX) and ferrochelatase (conversion of PpIX to haem) activities [34,35]. Iron metabolism has also been shown to be altered in tumor cells.…”

Section: Discussionmentioning

confidence: 95%

Enhancement of methyl-aminolevulinate photodynamic therapy by iron chelation with CP94: an in vitro investigation and clinical dose-escalating safety study for the treatment of nodular basal cell carcinoma

Pye

Campbell

Curnow

2008

J Cancer Res Clin Oncol

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Purpose:Methyl-aminolevulinate (MAL) photodynamic therapy (PDT) is a cancer therapy that combines the selective accumulation of a photosensitizer in tumor tissue with visible light (and tissue oxygen) to produce reactive oxygen species. This results in cellular damage and ablation of tumor tissue. Combining iron chelators with MAL has the potential to increase the accumulation of the photosensitizer protoporphyrin IX (PpIX) by reducing its bioconversion to heme. This paper investigates this method of enhancement both in vitro and for the first time clinically for the treatment of nodular basal cell carcinoma (BCC). Methods:Enhancement of MAL-induced PpIX accumulation by the iron chelator CP94 was quantified fluorometrically in human cultured cells (including three dermatological cell types). An open, dose-escalating, pilot study was then conducted in patients with nodular BCC, to determine the safety of this pharmacological modification. Results:Large enhancements in PpIX accumulation were observed in the cultured cells when coincubated with the iron chelator CP94. Clinically the addition of CP94 was found to be feasible and safe. In addition greater reductions in tumor depth were observed in the CP94 coincubated tumors. Conclusion:Iron chelation by CP94 is an effective enhancer of MAL-induced PpIX accumulation in vitro.

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Section: Discussionmentioning

confidence: 95%

Enhancement of methyl-aminolevulinate photodynamic therapy by iron chelation with CP94: an in vitro investigation and clinical dose-escalating safety study for the treatment of nodular basal cell carcinoma

Pye

Campbell

Curnow

2008

J Cancer Res Clin Oncol

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“…In these tumors, the hydrophilic 5-ALA is able to pass the disrupted blood-brain barrier (BBB) with an abnormal accumulation in the mitochondria and a tumor-specific porphyrin fluorescence [27,28]. Several additional mechanisms of PpIX accumulation in malignant glioma cells like reduced activity of ferrochelatase or decreased outflow of PpIX from the cells are reported [8,19].…”

Section: Discussionmentioning

confidence: 99%

The impact of fluorescence guidance on spinal intradural tumour surgery

Eicker

Floeth²,

Kamp

et al. 2013

Eur Spine J

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Purpose 5-Aminolevulinic acid (5-ALA)-based fluorescence-guided surgery was shown to be beneficial for cerebral malignant gliomas. Extension of this technique for resection of meningiomas and cerebral metastasis has been recently evaluated. Aim of the present study is to evaluate the impact of fluorescence-guided surgery in spinal tumor surgery. Methods Twenty-six patients with intradural spinal tumors were included in the study. 5-ALA was administered orally prior to the induction of anesthesia. Intraoperative, 440 nm fluorescence was applied after exploration of the tumor and, if positive, periodically during and at the end of resection to detect tumor-infiltrated sites. Results Tumors of WHO grade III and IV were found in five patients. In detail intra-or perimedullary metastasis of malignant cerebral gliomas was found including glioblastoma WHO grade IV (n = 2), anaplastic astrocytoma WHO grade III (n = 1), anaplastic oligoastrocytoma WHO grade III (n = 1). In addition, one patient suffered from a spinal drop metastasis of a cerebellar medulloblastoma WHO grade IV. Tumors of WHO grade I were diagnosed in 18 patients: Eight cases of meningioma (two recurrences), six cases of neurinoma, one neurofibroma, two ependymoma and one plexus papilloma. At least, benign pathologies were histologically proven in three patients. All four spinal metastasis of malignant glioma (100 %), seven of eight meningiomas (87.5 %) and one of two ependymoma (50 %) were found to be ALA-positive. Conclusion The present study demonstrates that spinal intramedullary gliomas and the majority of spinal intradural meningiomas are 5-ALA positive. As a surgical consequence, especially in intramedullary gliomas, the use of 5-ALA fluorescence seems to be beneficial.

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“…Zhang et al demonstrated that this signal has a lifetime on the order of 1 ns, thus it is due to emission by a tissue chromophore [15] but, to the best of our knowledge, an exact identification has not yet been made. Increased porphyrin production has been observed in multiple cancer types including cancers as diverse as breast, colon, endocrine, kidney, liver or lymphomas [18][19][20] tumor and normal fibrous tissue in the light scattering images was 1.09. In fact, only one of the specimens had a ratio between normal fibrous and tumor larger that 1.2 while half of the specimens had a ratio of ≈1 (see fig.…”

Section: Discussionmentioning

confidence: 99%

Investigation of near-infrared autofluorescence imaging for the detection of breast cancer

Demos

Bold

White

et al. 2005

IEEE J. Select. Topics Quantum Electron.

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Detection of breast cancer in fresh tissue obtained from surgery is investigated using Nearinfrared autofluorescence imaging under laser excitation at 532-nm and 632.8-nm. The differences in intensity between the three main components of breast tissue (cancer, fibrous and adipose) are estimated and compared to those obtained from cross-polarized light scattering images recorded under polarized illumination at 700-nm. The optical spectroscopic images for each tissue sample were subsequently compared with the histopathology slides. The experimental results indicate that the intensity of the near-infrared emission is considerably different in breast cancer compared to that of the adjacent non-neoplastic tissues (adipose and fibrous tissue). The experimental results suggest that 632.8-nm excitation offers key advantages compared to 532-nm excitation.

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Porphyrin metabolism in some malignant diseases

Cited by 143 publications

References 13 publications

Enhancement of methyl-aminolevulinate photodynamic therapy by iron chelation with CP94: an in vitro investigation and clinical dose-escalating safety study for the treatment of nodular basal cell carcinoma

Enhancement of methyl-aminolevulinate photodynamic therapy by iron chelation with CP94: an in vitro investigation and clinical dose-escalating safety study for the treatment of nodular basal cell carcinoma

The impact of fluorescence guidance on spinal intradural tumour surgery

Investigation of near-infrared autofluorescence imaging for the detection of breast cancer

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