Porous β‐tricalcium phosphate/collagen composites prepared in an alkaline condition

Abstract

Bone substitute materials with natural bone-like structure are considered to be favorable for bone regeneration. In this work, porous beta-tricalcium phosphate (beta-TCP)/collagen composite consisting of bone-like microstructural units was prepared using nanosized beta-TCP particles and alkaline-disassembled collagen. The resulting composite showed a good interconnecting porous structure with approximately 90% porosity and 100 approximately 300 microm pore size. The pore walls were dense, and the combination status of collagen and nanosized beta-TCP particles demonstrated that nanosized beta-TCP particles tightly connected collagen microfibrils as a bone-like microstructural unit. MTT and alkaline phosphatase (ALP) assays showed that the porous composite had enhanced effects on cellular proliferation and activity of osteoblast compared with a control of pure collagen. It is suggested that the adoption of nanosized beta-TCP particles is a main contribution to the formation of the composite with a bone-like microstructural unit, and the unique microstructure could be a main role for the composite to have the positive influence on osteoblast cell proliferation.

Biocompatibility evaluation of HDPE‐UHMWPE reinforced β‐TCP nanocomposites using highly purified human osteoblast cells

“…[3][4][5][6] There are several types of TCP: b, a, a 0 , and c. 7,8 Among them, b-TCP is widely used as bone grafts due to its simple and defined fabrication process. 9, 10 Zou et al 11 have reported that nanosized b-TCP composites could enhance the cellular proliferation and bioactivity of osteoblast cells, which is confirmed in many other studies. 12,13 However, the low tensile strength of b-TCP has limited the application of this biomaterial in loadbearing clinical applications.…”

Biodegradation Property of Beta‐Tricalcium Phosphate‐Collagen Composite in Accordance with Bone Formation: A Comparative Study with Bio‐Oss Collagen® in a Rat Critical‐Size Defect Model

“…Collagen is the most useful matrix for the fabrication of a composite comprising biomaterial and inorganic materials 1–3,27–29 . Many types of collagen‐based inorganic composite materials have been reported in the literature.…”

Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering

“…This paper focuses on the development of a novel composite scaffold for bone regeneration using the two major constituents of bone; collagen type 1 and hydroxyapatite (HA; Ca 10 (PO 4 ) 6 (OH) 2 ) nanoparticles (< 100 nm). Collagen is used extensively as a scaffold biomaterial due to its biocompatible and biodegradable properties [8][9][10][11][12]. From an orthopedic perspective however, collagen scaffolds are limited by their poor mechanical characteristics and for this reason many studies, including research in our lab, have combined collagen with calcium phosphates to improve their mechanical properties [13][14][15][16].…”