Porous Silicon Films Micropatterned with Bioelements as Supports for Mammalian Cells

Sweetman, Martin J.; Ronci, Maurizio; Ghaemi, Soraya Rasi; Craig, Jamie E; Voelcker, Nicolas H.

doi:10.1002/adfm.201102000

Cited by 42 publications

(24 citation statements)

References 72 publications

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“…In general, a high density of receptors enhances the transduced signal, and thus improves the biosensor sensitivity . Along with a fast fabrication process, pSi possesses a versatile surface chemistry that allows the incorporation of the desired chemical functionality into the porous material, mainly but not exclusively by hydrosilylation or silanization reactions …”

Section: Introductionmentioning

confidence: 99%

Porous Silicon Nanostructures as Effective Faradaic Electrochemical Sensing Platforms

Guo

Sharma

Toh

et al. 2019

Adv Funct Materials

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The electrochemical performance of porous silicon (pSi) stabilized via thermal decomposition of acetylene gas is investigated for the first time. In this study, pSi undergoes two thermal treatments at either 525 or 800 °C, which result in hydrogen-terminated thermally hydrocarbonized pSi (THCpSi) and hydroxylterminated thermally carbonized pSi (TCpSi), respectively, the latter upon dipping in hydrofluoric acid to activate the surface termination. Electrochemical characterization, using cyclic voltammetry, chronocoulometry, and electrochemical impedance spectroscopy in the presence of several redox pairs, [Fe(CN) 6 ] 3/4− , [Ru(NH 3 ) 6 ] 2/3+ , and hydroquinone/quinone, is used to demonstrate the versatility and high stability to degradation of carbon-stabilized pSi nanostructures and their excellent electrochemical performance. Added to the large surface area, adjustable pore morphology and tailorable surface chemistry of THCpSi and TCpSi, these nanostructures demonstrate fast electron-transfer kinetics, providing key advantages over conventional carbon electrodes. The versatile surface chemistry of THCpSi and TCpSi offer various possibilities to introduce multiple functional groups depending on the nature of the bioreceptor to be immobilized. For proof of principle, the use of a THCpSi-based immunosensor to detect MS2 bacteriophage is demonstrated by means of electrochemical impedance spectroscopy, showing a detection limit of 4.9 pfu mL −1 . Carbon-stabilized pSi structures represent a new class of nanostructured electrodes for biosensing applications.

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Section: Introductionmentioning

confidence: 99%

Porous Silicon Nanostructures as Effective Faradaic Electrochemical Sensing Platforms

Guo

Sharma

Toh

et al. 2019

Adv Funct Materials

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“…[9][10][11] Due to these qualities, PSi has been found to be a good material for use in vitro and in vivo biological applications. [12][13][14][15][16][17][18][19][20] Understanding the influence of topography on cell behavior is important to biomaterial and tissue engineering. Surface properties of biomaterials are associated with cell adhesion, cell growth, migration, and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Human aortic endothelial cell morphology influenced by topography of porous silicon substrates

et al. 2015

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Porous silicon has received much attention because of its optical properties and for its usefulness in cell-based biosensing, drug delivery, and tissue engineering applications. Surface properties of the biomaterial are associated with cell adhesion and with proliferation, migration, and differentiation. The present article analyzes the behavior of human aortic endothelial cells in macro- and nanoporous collagen-modified porous silicon samples. On both substrates, cells are well adhered and numerous. Confocal microscopy and scanning electron microscopy were employed to study the effects of porosity on the morphology of the cells. On macroporous silicon, filopodia is not observed but the cell spreads on the surface, increasing the lamellipodia surface which penetrates the macropore. On nanoporous silicon, multiple filopodia were found to branch out from the cell body. These results demonstrate that the pore size plays a key role in controlling the morphology and growth rate of human aortic endothelial cells, and that these forms of silicon can be used to control cell development in tissue engineering as well as in basic cell biology research.

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“…Porous silicon (pSi) is a nanostructured material that is being used widely in a range of disciplines including analytical chemistry and forensic science, due to its facile electrochemical fabrication procedures and ease of functionalization . pSi is easily transformed into MPs by sonicating or by ball‐milling “free‐standing” membranes of pSi .…”

Section: Figurementioning

confidence: 99%

“…[4] Porous silicon (pSi)i sananostructured material that is being used widely in ar ange of disciplines including analytical chemistry [5] and forensic science, [6] due to its facile electrochemicalf abrication procedures and ease of functionalization. [7] pSi is easily transformed into MPs by sonicating or by ball-milling "free-standing" membranes of pSi. [8] Since pSi is biocompatible and degrades into nontoxic silicic acid, pSi MPs have been routinelye xploited for in vivo applications involving drug delivery [9] and biosensing.…”

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confidence: 99%

Nanostructured Silicon‐Based Fingerprint Dusting Powders for Enhanced Visualization and Detection by Mass Spectrometry

Guinan

Kobus

et al. 2016

ChemPlusChem

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[a] Porous silicon microparticles (pSi MPs) functionalized with fluorescent dyes (lissamine and carboxy-5-fluorescein) and intrinsically luminescentp Si MPs weree xplored as novel fingerprint dusting powders. The versatility of luminescent pSi MPs is demonstrated through time-gated imaging of their long-lived (lifetime > 28 ms) near-IRemission, and mass spectrometry analysis of fingerprints dusted with luminescentpSi MPs to provide furtheri nformation on exogenous small molecules presenti n latent fingerprints.The visualization of latentf ingerprints (aka fingermarks) using dusting powders has been used for over 100 years to provide unique identifying information from individuals through variations in minutia such as ridge patterns.[1] Currently,t here exists ap lethora of dusting powders used to create contrast between the surface that the fingerprint is deposited onto and the dusted fingerprint. Carbon black [2] and TiO 2 microparticles (MPs) are commonly used fingerprint dusting powders and there is ag eneral consensus in the literature that the most effective dusting powders are 1-10 mmi ns ize. [3] In recentt imes, luminescent nanoparticles and MPs have been investigated for fingerprint detection techniques. [4] Porous silicon (pSi)i sananostructured material that is being used widely in ar ange of disciplines including analytical chemistry [5] and forensic science, [6] due to its facile electrochemicalf abrication procedures and ease of functionalization. [7] pSi is easily transformed into MPs by sonicating or by ball-milling "free-standing" membranes of pSi.[8] Since pSi is biocompatible and degrades into nontoxic silicic acid, pSi MPs have been routinelye xploited for in vivo applications involving drug delivery [9] and biosensing. [10] Recently,i ntrinsically luminescent pSi MPs were fabricated using ap H9.5 borate buffer.[11] The luminescence is attributed to quantum confinement effects in the silicon cores and to SiÀSiO 2 interfacial defects generated duringt he activation step.[11] Luminescent pSi feature ah igherp hotostability compared to fluorescently tagged powders.[11] In addition, theUV-absorbing properties and high surface area of pSi render it an attractive candidate for emerging nanostructured imaging mass spectrometry (NIMS) approaches, [12,13] NIMS is as oft ionization mass spectrometry technique using nanostructured substrates, including pSi, as energy receptacles for the desorption and ionization of small moleculess uch as illicit drugs. [14] This technique has been routinely exploited for imaging the spatial distribution of exogenous and endogenous fingerprint constituents. [13,15] For the first time, we presentt he application of fluorescently functionalized pSi MPs using as urfacec hemistry that can be used to covalently attach ar ange of fluorescent dyes.[16] Here, pSi MPs weref unctionalized with lissamine and carboxy-5-fluorescein, and dusted onto fingerprints deposited onto glass slides. In addition, we demonstrate the dual applications of pSi MPs as intrinsically luminesce...

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Porous Silicon Films Micropatterned with Bioelements as Supports for Mammalian Cells

Cited by 42 publications

References 72 publications

Porous Silicon Nanostructures as Effective Faradaic Electrochemical Sensing Platforms

Porous Silicon Nanostructures as Effective Faradaic Electrochemical Sensing Platforms

Human aortic endothelial cell morphology influenced by topography of porous silicon substrates

Nanostructured Silicon‐Based Fingerprint Dusting Powders for Enhanced Visualization and Detection by Mass Spectrometry

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