Porous silicon‐based scaffolds for tissue engineering and other biomedical applications

Coffer, Jeffery L.; Whitehead, Melanie A.; Nagesha, Dattatri; Mukherjee, Priyabrata; Akkaraju, Giridhar R.; Totolici, Mihaela; Saffie, Roghieh S.; Canham, Leigh

doi:10.1002/pssa.200461134

Cited by 141 publications

(87 citation statements)

References 9 publications

(5 reference statements)

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“…In Vitro SBF experiments are currently the most convenient and accurate method to test for the possible bioactivity and the bone bonding ability of a material [4,25]. It is not always as accurate as…”

Section: Tem Analysis After Sbfmentioning

confidence: 99%

Hydroxyapatite formation on metallurgical grade nanoporous silicon particles

2010

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Studies into bone-like apatite or hydroxyapatite (HA) growth on potential biomaterials when in contact with simulated body fluid (SBF) not only establish a general method for determining bioactivity but coincidently lead to the design of new bioactive materials in biomedical and tissue engineering fields. Previous studies of HA growth on porous silicon have examined electrochemically etched silicon substrates after immersion in a simulated body fluid. This study differs from previous work in that it focuses on characterising HA growth on chemically etched metallurgical grade nanoporous silicon particles. The porous silicon (PS) used in this study is comprised of nanosponge particles with disordered pore structures with pore sizes ranging up to 10nm on micron sized particles.The silicon particles are analysed before and after immersion into SBF using scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive x-ray (EDX) analysis and x-ray photoelectron spectroscopy (XPS). Results indicate that a HA layer forms on the surface of the nanosponge particles. Experimental analysis indicates that the morphology and calcium-tophosphorus ratio (Ca/P) verify the formation of crystalline HA on the nanoporous silicon particles.2

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Section: Tem Analysis After Sbfmentioning

confidence: 99%

Hydroxyapatite formation on metallurgical grade nanoporous silicon particles

2010

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“…Porous silicon (pSi) shows potential as a biocompatible scaffold for applications in orthopaedics (Li et al, 1998;Coffer et al, 2005;Whitehead et al, 2008;Anderson et al, 2010), oncology (Zhang et al, 2005;Mann et al, 2011;Park et al, 2011) and ophthalmology (Low et al, 2006;Cheng et al, 2008;Low et al, 2009;Kashanian et al, 2010). For use in ophthalmic implants designed to deliver drugs or cells to the eye, the advantages of pSi include its large surface area for drug-loading and cell attachment, and good ocular biocompatibility (Low et al, 2009;Kashanian et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

A novel pressed porous silicon-polycaprolactone composite as a dual-purpose implant for the delivery of cells and drugs to the eye

Irani

Tian

Wang

et al. 2015

Experimental Eye Research

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Abbreviations: BSS ophthalmic balanced salt solution; DMEM Dulbecco's Modified Eagle's Medium; EGF epidermal growth factor; FBS fetal bovine serum; FDA fluorescein diacetate; PBS phosphate buffered saline; PCL polycaprolactone; pSi nanostructured porous silicon; SEM scanning electron microscopy; TEM transmission electron microscopy. To investigate loading of large-molecule biologics, murine BALB/c 3T3 cells, responsive to epidermal growth factor, insulin and transferrin, were seeded on composite materials. The cells showed significantly more proliferation at 48 h when seeded on composites loaded with these biologics, than on unloaded composites. No cell proliferation was observed on PCL alone, indicating the biologics had loaded into the pSi microparticles. Drug release, measured by ELISA for insulin, indicated a burst followed by a slower, continuous release over six days. When implanted under the rat conjunctiva, the most promising composite material did not cause significant neovascularization but did elicit a macrophage and mild foreign body

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“…Its high surface area has been exploited in drug delivery 16 and biosensor applications 17,18 , where high levels of drug loading were reported. The effectiveness of pSi as a scaffold for bone regeneration also paves the way for tissue engineering applications 19 . pSi can be easily tailored to produce various pore sizes, depths and porosities through judicious choice of the etching conditions.…”

Section: Introductionmentioning

confidence: 99%

Preparation of two-directional gradient surfaces for the analysis of cell-surface interactions

Clements

Khung

Thissen

et al. 2007

BioMEMS and Nanotechnology III

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The ability to evaluate and control the cellular response to substrate materials is the key to a wide range of biomedical applications ranging from diagnostic tools to regenerative medicine. Gradient surfaces provide a simple and fast method for investigating optimal surface conditions for cellular responses such as attachment and growth. By using two orthogonal gradients on the same substrate, a large space of possible combinations can be screened simultaneously. Here, we have investigated the combination of a porous silicon (pSi) based topography gradient with a plasma polymer based thickness gradient. pSi was laterally anodised on a 1.5 x 2.5cm 2 silicon surface using hydrofluoric acid to form a pore size gradient along a single direction. The resulting pSi was characterised by SEM and AFM and pore sizes ranging from macro to mesoporous were found along the surface. Plasma polymerisation was used to form a thickness gradient orthogonal to the porous silicon gradient. Here, allylamine was chosen as the monomer and a mask placed over the substrate was used to achieve the thickness gradient. The analysis of this chemistry based gradient was carried out using profilometry and XPS. It is expected that orthogonal gradient substrates will be used increasingly for the in vitro screening of materials used in biomedical applications.

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Porous silicon‐based scaffolds for tissue engineering and other biomedical applications

Cited by 141 publications

References 9 publications

Hydroxyapatite formation on metallurgical grade nanoporous silicon particles

Hydroxyapatite formation on metallurgical grade nanoporous silicon particles

A novel pressed porous silicon-polycaprolactone composite as a dual-purpose implant for the delivery of cells and drugs to the eye

Preparation of two-directional gradient surfaces for the analysis of cell-surface interactions

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