Porous silicon as functionalized material for immunosensor application

Meskini, O.; Abdelghani, Adnane; Tlili, A.; M’gaieth, Ridha; Jaffrézic‐Renault, Nicole; Martelet, C.

doi:10.1016/j.talanta.2006.05.089

Cited by 59 publications

(22 citation statements)

References 10 publications

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“…However, a small SiH band remains at 615 cm -1 . After surface derivatization with APTS, a Si-O-Si band is observed at 1180 cm -1 , proving that a siloxane bond was formed between APTS and CoxPSi [19]. After biofunctionalization with immunoglobulins, two bands appear at 1535 and 1653 cm -1 related to the N-H and C=O bonds, respectively [28,29].…”

Section: Resultsmentioning

confidence: 95%

“…Another important difference is the absence of the Si-OH mode at 835-795 cm -1 [28] in ToxPSi, which is important for the following derivatization step. In the FTIR spectra of derivatized surfaces ( figure 8(b)), the Si-O-Si band at 1180 cm -1 , which proves the formation of the siloxane bond [19], is weaker in ToxPSi than in CoxPSi. Consistent with this result, there is a pronounced decrease in the Si-OH and SiO peak areas after derivatization of CoxPSi, which is not observed for ToxPSi.…”

Section: Resultsmentioning

confidence: 99%

“…Currently, thermal oxidation is the most popular stabilization process for PSi. Whereas such stabilization is crucial for optoelectronic technologies, its necessity for biological applications is yet uncertain [15][16][17][18][19]. In particular, it is unclear whether the direct derivatization of a fresh PSi surface is adequate for subsequent biological experiments.…”

Section: Introductionmentioning

confidence: 99%

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Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Naveas

Costa

Gallach

et al. 2012

Science and Technology of Advanced Materials

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Porous silicon (PSi) is widely used in biological experiments, owing to its biocompatibility and well-established fabrication methods that allow tailoring its surface. Nevertheless, there are some unresolved issues such as deciding whether the stabilization of PSi is necessary for its biological applications and evaluating the effects of PSi stabilization on the surface biofunctionalization with proteins. In this work we demonstrate that non-stabilized PSi is prone to detachment owing to the stress induced upon biomolecular adsorption. Biofunctionalized non-stabilized PSi loses the interference properties characteristic of a thin film, and groove-like structures resulting from a final layer collapse were observed by scanning electron microscopy. Likewise, direct PSi derivatization with 3-aminopropyl-triethoxysilane (APTS) does not stabilize PSi against immunoglobulin biofunctionalization. To overcome this problem, we developed a simple chemical process of stabilizing PSi (CoxPSi) for biological applications, which has several advantages over thermal stabilization (ToxPSi). The process consists of chemical oxidation in H 2 O 2 , surface derivatization with APTS and a curing step at 120• C. This process offers integral homogeneous PSi morphology, hydrophilic surface termination (contact angle θ = 26• ) and highly efficient derivatized and biofunctionalized PSi surfaces (six times more efficient than ToxPSi). All these features are highly desirable for biological applications, such as biosensing, where our results can be used for the design and optimization of the biomolecular immobilization cascade on PSi surfaces.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Naveas

Costa

Gallach

et al. 2012

Science and Technology of Advanced Materials

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“…It is possible to use a physical or chemical method to overcome this drawback. A commonly used chemical method is the functionalization of the surface of silicon nanocrystals in the porous layers [8,9]. It is a chemical process to create the new chemical bond that combines selectively with molecules of the studied substances.…”

Section: Vietnam Academy Of Science and Technologymentioning

confidence: 99%

Nano porous silicon microcavity sensor for determination organic solvents and pesticide in water

Pham

Nguyen

Bui

2014

Adv. Nat. Sci: Nanosci. Nanotechnol.

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In this paper we present a sensing method using nano-porous silicon microcavity sensor, which was developed in order to obtain simultaneous determination of two volatile substances with different solvent concentrations as well as very low pesticide concentration in water. The temperature of the solution and the velocity of the air stream flowing through the solution have been used to control the response of the sensor for different solvent solutions. We study the dependence of the cavity-resonant wavelength shift on solvent concentration, velocity of the airflow and solution temperature. The wavelength shift depends linearly on concentration and increases with solution temperature and velocity of the airflow. The dependence of the wavelength shift on the solution temperature in the measurement contains properties of the temperature dependence of the solvent vapor pressure, which characterizes each solvent. As a result, the dependence of the wavelength shift on the solution temperature discriminates between solutions of ethanol and acetone with different concentrations. This suggests a possibility for the simultaneous determination of the volatile substances and their concentrations. On the other hand, this method is able to detect the presence of atrazine pesticide by the shift of the resonant wavelength, with good sensitivity (0.3 nm pg −1 ml) and limit of detection (LOD) (0.8-1.4 pg ml −1 ), that we tested for concentrations in the range from 2.15 to 21.5 pg ml −1 , which is the range useful for monitoring acceptable water for human consumption.

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“…It is possible to use a physical or chemical method to overcome this drawback. A commonly used chemical method is the functionalization of the surface of silicon nanocrystals in the porous layers [9,10]. It is a chemical process to create the new chemical bond that combines selectively with molecules of the studied substances.…”

Section: Introductionmentioning

confidence: 99%

A Vapor Sensor Based on a Porous Silicon Microcavity for the Determination of Solvent Solutions

Bui

Nguyen

Pham

et al. 2014

Journal of the Optical Society of Korea

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A porous silicon microcavity (PSMC) sensor has been made for vapors of solvent solutions, and a method has been developed in order to obtain simultaneous determination of two volatile substances with different concentrations. In our work, the temperature of the solution and the velocity of the air stream flowing through the solution have been used to control the response of the sensor for ethanol and acetone solutions. We study the dependence of the cavity-resonant wavelength shift on solvent concentration, velocity of the airflow and solution temperature. The wavelength shift depends linearly on concentration and increases with solution temperature and velocity of the airflow. The dependence of the wavelength shift on the solution temperature in the measurement contains properties of the temperature dependence of the solvent vapor pressure, which characterizes each solvent. As a result, the dependence of the wavelength shift on the solution temperature discriminates between solutions of ethanol and acetone with different concentrations. This suggests a possibility for the simultaneous determination of the volatile substances and their concentrations.

show abstract

Porous silicon as functionalized material for immunosensor application

Cited by 59 publications

References 10 publications

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Nano porous silicon microcavity sensor for determination organic solvents and pesticide in water

A Vapor Sensor Based on a Porous Silicon Microcavity for the Determination of Solvent Solutions

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