Porous silica particles grafted with an amphiphilic side‐chain polymer as a stationary phase in reversed‐phase high‐performance liquid chromatography

Abstract

The amphiphilic polymer-grafted silica was newly prepared as a stationary phase in high-performance liquid chromatography. Poly(4-vinylpyridine) with a trimethoxysilyl group at one end was grafted onto porous silica particles and the pyridyl side chains were quaternized with 1-bromooctadecane. The obtained poly(octadecylpyridinium)-grafted silica was characterized by elemental analysis, diffuse reflectance infrared Fourier transform spectroscopy and Brunauer-Emmett-Teller analysis. The degree of quaternization of the pyridyl groups on the obtained stationary phase was estimated to be 70%. The selective retention behaviors of polycyclic aromatic hydrocarbons including some positional isomers were investigated using poly(octadecylpyridinium)-grafted silica as an amphiphilic polymer stationary phase in high-performance liquid chromatography and results were compared with commercially available polymeric octadecylated silica and phenyl-bonded silica columns. The results indicate that the selectivity toward polycyclic aromatic hydrocarbons exhibited by the amphiphilic polymer stationary phase is higher than the corresponding selectivity exhibited by a conventional phenyl-bonded silica column. However, compared with the polymeric octadecylated silica phase, the new stationary phase presents similar retention behavior for polycyclic aromatic hydrocarbons but different retention behavior particularly for positional isomers of disubstituted benzenes as the aggregation structure of amphiphilic polymers on the surface of silica substrate has been altered during mobile phase variation.

Molecular Shape Selectivity for Polycyclic Aromatic Compoundson a Poly(4-vinylpyridine) Stationary Phase in Liquid Chromatography

“…Octadecylsilica (ODS) is widely employed as a stationary phase in LC because of the several advantageous features such as a good versatility, high separation performance, and significantly wide commercial availability with a reasonable cost [11][12][13]. However, there are still many types of complex mixtures, consisted of many closely-related components, that are quite difficult to be separated completely by single separation on typical ODS phases [14][15][16][17][18][19][20][21].…”

Preparation and characterization of a novel hydrophilic interaction/ion exchange mixed‐mode chromatographic stationary phase with pyridinium‐based zwitterionic polymer‐grafted porous silica

“…We have been developing polymer‐grafted porous silica particles for HPLC stationary phases that exhibit a wide range of separation modes . Very recently, novel porous silica microspheres (Sil) modified with poly(4‐vinylpyridinium salt)s (VP n ) having chemically tunable side chains were reported for use as the stationary phase in liquid chromatography; alkyl halides with diverse functional groups can be introduced into the polymer side chains via quaternization of the pyridyl group . Side‐chain‐functionalized VP n ‐grafted silica showed unique abilities to separate polycyclic aromatic hydrocarbons, aromatic positional isomers, and bioactive compounds.…”

“…Side‐chain‐functionalized VP n ‐grafted silica showed unique abilities to separate polycyclic aromatic hydrocarbons, aromatic positional isomers, and bioactive compounds. While simple alkyl chains such as methyl and octadecyl halides were used to functionalize the polymer side chains in our previous study , here we create zwitterionic functional side chains by introducing alkyl halides bearing anionic sulfonates via the quaternization reaction of VP n (Figure A). It is expected that these zwitterionic‐polymer‐functionalized microspheres will exhibit unique separation performance because the mobilities of zwitterionic moieties on the flexible polymer chain are higher than those stabilized directly on the substrate surface and should facilitate better interactions with the analytes (Figure B).…”

Rapid determination of trace polycyclic aromatic hydrocarbons in particulate matter using accelerated solvent extraction followed by ultra high performance liquid chromatography with fluorescence detection

“…PAHs have been determined using GC, GC–MS, HPLC, and UHPLC . Compared with GC and GC−MS, HPLC, or UHPLC is more suitable for the separation of PAHs of high boiling points with isomers included, and has therefore been developed by coupling it to the detectors such as the fluorescence detector (FLD), the diode array detector (DAD), and the UV detector (UVD).…”